Alternaria alternata virus 1
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3IXK4|B3IXK4_9VIRU Uncharacterized protein OS=Alternaria alternata virus 1 OX=483537 PE=4 SV=1
MM1 pKa = 7.64 FDD3 pKa = 3.89 SFCSFPAFVPFVLDD17 pKa = 3.75 DD18 pKa = 3.62 FSGRR22 pKa = 11.84 VSAPALLTVQATGGMKK38 pKa = 10.33 DD39 pKa = 3.38 GRR41 pKa = 11.84 QMATASIAFSGAVPSVDD58 pKa = 4.93 ALDD61 pKa = 5.93 DD62 pKa = 3.98 DD63 pKa = 4.46 TLSCGVGRR71 pKa = 11.84 YY72 pKa = 6.97 IHH74 pKa = 6.56 HH75 pKa = 5.53 VHH77 pKa = 5.82 VDD79 pKa = 3.27 KK80 pKa = 11.67 VSFCGGAVCDD90 pKa = 5.81 KK91 pKa = 7.39 EE92 pKa = 5.09 TCCSGCGVSSVRR104 pKa = 11.84 PDD106 pKa = 3.32 PEE108 pKa = 5.46 GGFFSSASLGALVSAGLVEE127 pKa = 5.68 AGLEE131 pKa = 4.04 DD132 pKa = 3.84 EE133 pKa = 4.96 RR134 pKa = 11.84 CTVMVSFGGLRR145 pKa = 11.84 IPLDD149 pKa = 3.33 ATGGVVPHH157 pKa = 6.38 SRR159 pKa = 11.84 ALDD162 pKa = 3.62 EE163 pKa = 4.47 AALGVFLPPPQTSDD177 pKa = 3.35 PGDD180 pKa = 3.23 VAVPNEE186 pKa = 4.2 PCDD189 pKa = 4.05 EE190 pKa = 4.54 EE191 pKa = 4.9 IKK193 pKa = 10.12 TLADD197 pKa = 3.64 GLLSHH202 pKa = 6.75 AVEE205 pKa = 4.83 LGMPEE210 pKa = 3.81 QAAEE214 pKa = 4.04 ASVVVPLPPPPPDD227 pKa = 4.4 PEE229 pKa = 4.68 DD230 pKa = 4.03 LPGSAMNLGTLGGVLAAIADD250 pKa = 3.87 GSLVRR255 pKa = 11.84 EE256 pKa = 4.39 IKK258 pKa = 9.96 TYY260 pKa = 10.76 DD261 pKa = 3.34 PPAGGFEE268 pKa = 4.41 GPRR271 pKa = 11.84 SEE273 pKa = 4.18 VLEE276 pKa = 5.63 RR277 pKa = 11.84 RR278 pKa = 11.84 LQWLNALGSLSDD290 pKa = 3.24 SCYY293 pKa = 11.1 SVVADD298 pKa = 3.99 AGWYY302 pKa = 9.88 RR303 pKa = 11.84 LLLPFAHH310 pKa = 6.72 WQKK313 pKa = 10.92 QLGLLSGRR321 pKa = 11.84 EE322 pKa = 3.85 MDD324 pKa = 4.67 LLLGGLSRR332 pKa = 11.84 VKK334 pKa = 10.57 DD335 pKa = 3.33 PRR337 pKa = 11.84 LRR339 pKa = 11.84 ADD341 pKa = 3.99 RR342 pKa = 11.84 YY343 pKa = 9.49 LHH345 pKa = 6.35 FLRR348 pKa = 11.84 TVVGASAGGVGDD360 pKa = 4.34 PPWAYY365 pKa = 10.41 GSVANFQVGMYY376 pKa = 10.03 RR377 pKa = 11.84 LLWPARR383 pKa = 11.84 GAKK386 pKa = 10.09 VGVGTSAA393 pKa = 3.54
Molecular weight: 40.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.607
IPC2_protein 4.635
IPC_protein 4.596
Toseland 4.431
ProMoST 4.736
Dawson 4.571
Bjellqvist 4.711
Wikipedia 4.469
Rodwell 4.444
Grimsley 4.342
Solomon 4.571
Lehninger 4.52
Nozaki 4.685
DTASelect 4.889
Thurlkill 4.457
EMBOSS 4.495
Sillero 4.724
Patrickios 3.732
IPC_peptide 4.571
IPC2_peptide 4.711
IPC2.peptide.svr19 4.674
Protein with the highest isoelectric point:
>tr|B3IXK2|B3IXK2_9VIRU Uncharacterized protein OS=Alternaria alternata virus 1 OX=483537 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 SDD4 pKa = 3.25 KK5 pKa = 10.83 TLLLLGFRR13 pKa = 11.84 NAAWLRR19 pKa = 11.84 AQGSHH24 pKa = 7.38 PDD26 pKa = 3.54 LGSIPKK32 pKa = 9.84 RR33 pKa = 11.84 AFVPEE38 pKa = 4.14 PKK40 pKa = 10.35 EE41 pKa = 4.08 IDD43 pKa = 3.14 ACLQGIEE50 pKa = 4.96 DD51 pKa = 3.81 GWGDD55 pKa = 3.67 GAFWCQTVVGSGFPVFFVLSDD76 pKa = 2.89 VSRR79 pKa = 11.84 NVFCHH84 pKa = 6.02 SFAEE88 pKa = 4.36 VGGDD92 pKa = 3.35 VSVVSFGLTEE102 pKa = 4.26 ARR104 pKa = 11.84 SQLYY108 pKa = 9.81 RR109 pKa = 11.84 KK110 pKa = 9.67 AQVCVVPKK118 pKa = 10.43 ARR120 pKa = 11.84 PAVAYY125 pKa = 10.29 HH126 pKa = 7.0 RR127 pKa = 11.84 DD128 pKa = 3.23 LSAVARR134 pKa = 11.84 VLRR137 pKa = 11.84 FGVLNANNKK146 pKa = 9.77 LCDD149 pKa = 3.58 AYY151 pKa = 9.96 TDD153 pKa = 4.46 FVRR156 pKa = 11.84 SPVARR161 pKa = 11.84 TEE163 pKa = 4.25 EE164 pKa = 4.04 TWGLLGGYY172 pKa = 7.44 PWRR175 pKa = 11.84 DD176 pKa = 2.8 LDD178 pKa = 4.3 ALVASSTRR186 pKa = 11.84 AEE188 pKa = 4.19 YY189 pKa = 10.84 DD190 pKa = 2.98 RR191 pKa = 11.84 DD192 pKa = 3.76 ADD194 pKa = 3.89 TKK196 pKa = 11.21 LVGFLDD202 pKa = 3.87 RR203 pKa = 11.84 LWGKK207 pKa = 10.27 KK208 pKa = 9.17 RR209 pKa = 11.84 GKK211 pKa = 10.58 GLGRR215 pKa = 11.84 QDD217 pKa = 2.77 KK218 pKa = 8.97 KK219 pKa = 9.6 TRR221 pKa = 11.84 AKK223 pKa = 10.85 AAMAWGDD230 pKa = 3.44 ANLWTRR236 pKa = 11.84 EE237 pKa = 3.74 VSFKK241 pKa = 10.38 RR242 pKa = 11.84 RR243 pKa = 11.84 HH244 pKa = 5.69 YY245 pKa = 10.67 YY246 pKa = 10.68 DD247 pKa = 4.23 LATFLCTMDD256 pKa = 4.49 EE257 pKa = 4.33 VWGDD261 pKa = 3.31 RR262 pKa = 11.84 AVAMQGGATSKK273 pKa = 10.63 EE274 pKa = 3.62 ADD276 pKa = 3.77 VQCLSFRR283 pKa = 11.84 KK284 pKa = 9.21 AVRR287 pKa = 11.84 MVLSDD292 pKa = 5.16 LIFGPATYY300 pKa = 10.34 EE301 pKa = 3.89 RR302 pKa = 11.84 IYY304 pKa = 10.6 LTVFWIFSTRR314 pKa = 11.84 WWPSLVPVLLHH325 pKa = 6.46 FGSLGMSDD333 pKa = 4.82 DD334 pKa = 4.62 EE335 pKa = 4.3 YY336 pKa = 10.28 TAVHH340 pKa = 6.64 KK341 pKa = 10.87 EE342 pKa = 3.63 MTAVVTSTWMVPGTCRR358 pKa = 11.84 QHH360 pKa = 5.91 QFSANFLNAEE370 pKa = 4.57 DD371 pKa = 3.7 LTGWSDD377 pKa = 3.47 RR378 pKa = 11.84 DD379 pKa = 3.67 SLKK382 pKa = 11.19 GGVCAEE388 pKa = 4.17 IIKK391 pKa = 10.21 FALATFEE398 pKa = 4.31 YY399 pKa = 10.31 KK400 pKa = 10.58 ADD402 pKa = 3.56 VRR404 pKa = 11.84 DD405 pKa = 3.86 GGGEE409 pKa = 3.63 AGIPIRR415 pKa = 11.84 GKK417 pKa = 9.45 EE418 pKa = 4.13 GSPEE422 pKa = 4.05 EE423 pKa = 3.83 YY424 pKa = 10.18 LGRR427 pKa = 11.84 YY428 pKa = 7.56 RR429 pKa = 11.84 QAMYY433 pKa = 11.15 DD434 pKa = 3.78 LLRR437 pKa = 11.84 PMYY440 pKa = 10.62 ARR442 pKa = 11.84 YY443 pKa = 9.3 APNMVDD449 pKa = 4.04 LQAHH453 pKa = 5.06 MEE455 pKa = 4.09 KK456 pKa = 10.51 RR457 pKa = 11.84 MAWMSGGAVGRR468 pKa = 11.84 RR469 pKa = 11.84 AKK471 pKa = 10.32 EE472 pKa = 3.52 LLGPGIAPPGSSKK485 pKa = 11.07 AYY487 pKa = 8.2 VAARR491 pKa = 11.84 ADD493 pKa = 3.44 ISQLTRR499 pKa = 11.84 DD500 pKa = 3.45 WGEE503 pKa = 3.46 MRR505 pKa = 11.84 IEE507 pKa = 3.98 VGGKK511 pKa = 8.57 GNEE514 pKa = 4.03 RR515 pKa = 11.84 GSEE518 pKa = 3.96 RR519 pKa = 11.84 TLLATDD525 pKa = 4.29 LRR527 pKa = 11.84 DD528 pKa = 3.66 QVSEE532 pKa = 4.39 SYY534 pKa = 10.83 LLHH537 pKa = 6.56 AFKK540 pKa = 10.77 NRR542 pKa = 11.84 YY543 pKa = 8.45 GLIGVDD549 pKa = 3.33 VGEE552 pKa = 4.82 TPVEE556 pKa = 3.84 MFKK559 pKa = 10.72 RR560 pKa = 11.84 HH561 pKa = 4.39 VTVASATDD569 pKa = 3.55 VPLVHH574 pKa = 7.01 HH575 pKa = 6.63 GRR577 pKa = 11.84 DD578 pKa = 3.6 KK579 pKa = 11.13 KK580 pKa = 11.24 VLAAWDD586 pKa = 3.71 YY587 pKa = 11.34 SKK589 pKa = 10.67 WDD591 pKa = 3.49 HH592 pKa = 6.43 HH593 pKa = 7.34 VMLAEE598 pKa = 3.87 RR599 pKa = 11.84 LILVEE604 pKa = 4.05 VMRR607 pKa = 11.84 KK608 pKa = 9.16 LVLEE612 pKa = 4.64 FVQRR616 pKa = 11.84 PDD618 pKa = 2.94 VRR620 pKa = 11.84 EE621 pKa = 3.93 DD622 pKa = 3.16 MLRR625 pKa = 11.84 EE626 pKa = 4.07 LEE628 pKa = 4.24 VLEE631 pKa = 4.53 SSHH634 pKa = 5.59 RR635 pKa = 11.84 TAIYY639 pKa = 9.98 RR640 pKa = 11.84 SRR642 pKa = 11.84 AFADD646 pKa = 3.29 AKK648 pKa = 10.45 YY649 pKa = 8.83 TDD651 pKa = 3.93 QVDD654 pKa = 3.47 SLIRR658 pKa = 11.84 EE659 pKa = 4.26 GVAAGFKK666 pKa = 11.11 GEE668 pKa = 4.05 VQRR671 pKa = 11.84 LSADD675 pKa = 3.18 QVRR678 pKa = 11.84 ITNYY682 pKa = 10.27 AGQQSGRR689 pKa = 11.84 RR690 pKa = 11.84 STLEE694 pKa = 3.6 SNTFYY699 pKa = 11.23 SRR701 pKa = 11.84 ARR703 pKa = 11.84 LLVRR707 pKa = 11.84 DD708 pKa = 4.43 AEE710 pKa = 4.25 LLDD713 pKa = 3.93 AEE715 pKa = 4.32 RR716 pKa = 11.84 SIYY719 pKa = 10.45 LLNRR723 pKa = 11.84 ADD725 pKa = 5.03 DD726 pKa = 3.46 VMEE729 pKa = 4.91 IYY731 pKa = 10.32 RR732 pKa = 11.84 AWEE735 pKa = 3.72 HH736 pKa = 6.0 ARR738 pKa = 11.84 NAIDD742 pKa = 4.24 VMLLQGHH749 pKa = 6.65 KK750 pKa = 10.49 ANKK753 pKa = 8.77 KK754 pKa = 7.94 KK755 pKa = 10.5 QVVQVRR761 pKa = 11.84 TGVYY765 pKa = 10.09 FRR767 pKa = 11.84 ILYY770 pKa = 10.65 ANGSMRR776 pKa = 11.84 GFPPRR781 pKa = 11.84 AVYY784 pKa = 10.5 ACASAGPSMAASGGFDD800 pKa = 3.15 PVEE803 pKa = 4.21 RR804 pKa = 11.84 LSSLSGALDD813 pKa = 3.24 RR814 pKa = 11.84 LARR817 pKa = 11.84 RR818 pKa = 11.84 GSGYY822 pKa = 10.04 YY823 pKa = 9.13 VARR826 pKa = 11.84 ALYY829 pKa = 10.21 FEE831 pKa = 5.3 AEE833 pKa = 4.12 DD834 pKa = 4.07 YY835 pKa = 11.05 YY836 pKa = 11.5 RR837 pKa = 11.84 DD838 pKa = 3.68 VRR840 pKa = 11.84 VQLAAKK846 pKa = 9.07 ADD848 pKa = 3.37 KK849 pKa = 9.23 YY850 pKa = 9.85 TRR852 pKa = 11.84 FTIPRR857 pKa = 11.84 EE858 pKa = 4.02 VLRR861 pKa = 11.84 ASPDD865 pKa = 3.31 LGGCGVLPPGCYY877 pKa = 10.06 DD878 pKa = 3.27 YY879 pKa = 11.01 DD880 pKa = 3.96 YY881 pKa = 10.9 TIKK884 pKa = 10.55 CSNIKK889 pKa = 10.29 GPNEE893 pKa = 3.86 QLWQAIRR900 pKa = 11.84 EE901 pKa = 4.17 RR902 pKa = 11.84 LEE904 pKa = 3.71 KK905 pKa = 9.87 RR906 pKa = 11.84 HH907 pKa = 5.91 KK908 pKa = 10.51 FRR910 pKa = 11.84 GLADD914 pKa = 3.78 LQGSAGRR921 pKa = 11.84 AFAHH925 pKa = 6.45 DD926 pKa = 4.12 VPLEE930 pKa = 4.2 VPDD933 pKa = 5.07 KK934 pKa = 11.09 AWQQWSKK941 pKa = 9.4 RR942 pKa = 11.84 WRR944 pKa = 11.84 GDD946 pKa = 2.74 RR947 pKa = 11.84 AAQDD951 pKa = 3.45 GRR953 pKa = 11.84 GEE955 pKa = 4.13 LLRR958 pKa = 11.84 AKK960 pKa = 9.88 MMAKK964 pKa = 9.41 LWRR967 pKa = 11.84 YY968 pKa = 8.01 KK969 pKa = 10.53 RR970 pKa = 11.84 SWHH973 pKa = 6.39 RR974 pKa = 11.84 DD975 pKa = 3.0 KK976 pKa = 11.52 KK977 pKa = 10.29 GDD979 pKa = 3.28 TWGLEE984 pKa = 4.36 GFGHH988 pKa = 7.59 LLSLPVDD995 pKa = 4.39 FFNIAWRR1002 pKa = 11.84 MLRR1005 pKa = 11.84 DD1006 pKa = 3.69 CPGTGEE1012 pKa = 4.34 VDD1014 pKa = 3.37 RR1015 pKa = 11.84 LMKK1018 pKa = 10.44 GVQGPPAEE1026 pKa = 4.31 GMLAKK1031 pKa = 10.37 LWYY1034 pKa = 10.47 GLGQDD1039 pKa = 4.1 VISVLGRR1046 pKa = 11.84 DD1047 pKa = 3.64 VLEE1050 pKa = 4.88 VIQSASAPGRR1060 pKa = 11.84 EE1061 pKa = 4.98 FAAEE1065 pKa = 4.12 WGWLPLDD1072 pKa = 3.56 VRR1074 pKa = 11.84 EE1075 pKa = 4.15 KK1076 pKa = 10.64 FLRR1079 pKa = 11.84 GHH1081 pKa = 7.52 LGPAGAWMNLIPASFAPWLNQAVNFVLFQAFATGVRR1117 pKa = 11.84 YY1118 pKa = 9.9 RR1119 pKa = 11.84 GSHH1122 pKa = 5.45 WLLCVRR1128 pKa = 11.84 QAITGAISRR1137 pKa = 11.84 QFVNSRR1143 pKa = 11.84 PWLMLHH1149 pKa = 7.02
Molecular weight: 129.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.726
IPC2_protein 8.639
IPC_protein 8.565
Toseland 9.077
ProMoST 9.033
Dawson 9.443
Bjellqvist 9.311
Wikipedia 9.648
Rodwell 9.575
Grimsley 9.516
Solomon 9.472
Lehninger 9.428
Nozaki 9.355
DTASelect 9.224
Thurlkill 9.297
EMBOSS 9.56
Sillero 9.458
Patrickios 4.762
IPC_peptide 9.458
IPC2_peptide 8.053
IPC2.peptide.svr19 7.75
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3145
393
1149
786.3
85.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.493 ± 0.367
1.304 ± 0.229
6.9 ± 0.328
5.024 ± 0.163
3.688 ± 0.217
10.588 ± 0.771
2.194 ± 0.112
2.13 ± 0.298
3.084 ± 0.663
9.507 ± 0.816
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.135
1.653 ± 0.322
5.215 ± 0.842
2.576 ± 0.289
8.172 ± 0.628
6.709 ± 0.432
3.847 ± 0.252
9.253 ± 0.565
2.448 ± 0.275
2.925 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here