Salmonella bongori
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A447M053|A0A447M053_SALBN Holo-[acyl-carrier-protein] synthase OS=Salmonella bongori OX=54736 GN=acpS PE=3 SV=1
MM1 pKa = 6.79 ITVIAIDD8 pKa = 3.47 AAGNASPVSDD18 pKa = 3.24 SVNFVVDD25 pKa = 3.89 TTPPLAPVITSVSDD39 pKa = 3.52 DD40 pKa = 3.59 QAPGLGTIATGQNTNDD56 pKa = 3.57 PTPTFSGTAEE66 pKa = 4.03 AGATITLYY74 pKa = 11.12 EE75 pKa = 4.43 NGTVIGTTTAQPDD88 pKa = 4.42 GAWSVPTSTLASGTHH103 pKa = 6.44 VITAVATDD111 pKa = 3.5 TAGNDD116 pKa = 3.92 SPNSTAFTLTVDD128 pKa = 3.85 TTAPQTPILTSVVDD142 pKa = 4.27 DD143 pKa = 3.81 VAGGVTGNLANGQITNDD160 pKa = 3.47 NRR162 pKa = 11.84 PTLNGTAEE170 pKa = 4.26 AGSVVSIYY178 pKa = 11.08 DD179 pKa = 3.44 GDD181 pKa = 3.95 TLLGVTSANTGGAWSFTPTTGLNDD205 pKa = 3.54 GTHH208 pKa = 5.22 TLKK211 pKa = 9.55 VTATDD216 pKa = 3.52 PAGNVSPATSGFTIVVDD233 pKa = 3.97 TLAPTLPLITSIIDD247 pKa = 3.31 DD248 pKa = 3.77 VLNNTGAISNGQSTNDD264 pKa = 3.58 TQPTLNGTAEE274 pKa = 4.2 ANSAVNIFDD283 pKa = 3.8 NGTLVATVNASANGNWSWTPTVEE306 pKa = 4.67 LGQGSHH312 pKa = 6.9 AYY314 pKa = 9.73 SVSAADD320 pKa = 3.33 AAGNISAASQLTTIIVDD337 pKa = 3.79 TLAPGTPGNLVINATGNRR355 pKa = 11.84 VTGTAEE361 pKa = 4.09 AGSMVTITSDD371 pKa = 3.07 TGVVLGTATADD382 pKa = 3.5 GTGSFTATLTPAQTNGQPLLAFAQDD407 pKa = 3.34 KK408 pKa = 11.01 AGNTGIAARR417 pKa = 11.84 FTAPDD422 pKa = 3.4 TRR424 pKa = 11.84 VPEE427 pKa = 4.26 APVITNIVDD436 pKa = 3.42 DD437 pKa = 4.05 VGIYY441 pKa = 9.83 TGVIANGQVTNDD453 pKa = 3.81 ARR455 pKa = 11.84 PTLNGTAQAGATVSIYY471 pKa = 11.32 NNGALLGITTANASGNWSFTPTGNLTEE498 pKa = 4.61 GSHH501 pKa = 6.73 AFTATATNANGTGSVSTAATVMVDD525 pKa = 3.1 TLAPGTPSGTLSADD539 pKa = 3.37 GGSLSGLAEE548 pKa = 4.49 ANSTVTVTLAGGVTLTTTAGSNGAWSLTLPTKK580 pKa = 10.44 QIEE583 pKa = 4.44 GQLINVTATDD593 pKa = 3.5 AAGNASGTLGITAPVLPLAARR614 pKa = 11.84 DD615 pKa = 4.2 NITSLDD621 pKa = 3.9 LTSTAATSTQHH632 pKa = 5.66 YY633 pKa = 9.99 SDD635 pKa = 4.27 YY636 pKa = 11.63 GLLLVGALGNVASVLGNDD654 pKa = 3.59 TAQVEE659 pKa = 4.44 FTIAEE664 pKa = 4.54 GGTGDD669 pKa = 3.66 VTIDD673 pKa = 3.14 AAATGIVLSLLSTQEE688 pKa = 3.94 IVVQRR693 pKa = 11.84 YY694 pKa = 7.26 DD695 pKa = 3.21 TSLGAWTTIVNTAVGDD711 pKa = 3.91 FANLLTLTGSGVTLNLSGLSEE732 pKa = 3.9 GQYY735 pKa = 10.58 RR736 pKa = 11.84 VLTYY740 pKa = 9.12 NTSLLATGSYY750 pKa = 10.1 TSLDD754 pKa = 3.32 VDD756 pKa = 3.58 VHH758 pKa = 4.66 QTSAGIISGPTINTGNVMADD778 pKa = 3.28 DD779 pKa = 4.18 TAPTGTTVTAITNASGISSPVGAGGVDD806 pKa = 2.88 IQGQYY811 pKa = 8.41 GTLHH815 pKa = 6.56 INQDD819 pKa = 2.73 GSYY822 pKa = 9.43 TYY824 pKa = 10.99 TLTNPTAGYY833 pKa = 8.61 GHH835 pKa = 7.14 KK836 pKa = 10.71 EE837 pKa = 3.72 NFTYY841 pKa = 10.21 TITQNGVGSSTAQLVINLGPAPVPGSVVATDD872 pKa = 3.83 NNASLVFDD880 pKa = 3.12 THH882 pKa = 7.16 VNYY885 pKa = 10.43 VNNGPSTQSGVTVLSVGLGNVLSANLLDD913 pKa = 5.77 DD914 pKa = 3.61 MTNPILFNVEE924 pKa = 3.6 EE925 pKa = 4.77 GATRR929 pKa = 11.84 TLTLQGTVGGVSLVSTFDD947 pKa = 3.41 LYY949 pKa = 11.31 VYY951 pKa = 10.37 RR952 pKa = 11.84 FNDD955 pKa = 3.53 VIQQYY960 pKa = 8.12 EE961 pKa = 3.84 QFRR964 pKa = 11.84 VQKK967 pKa = 10.52 GWINTLLLAGQSQPLTLTLPGGEE990 pKa = 4.13 YY991 pKa = 10.81 LFVLNTASGISVLTGYY1007 pKa = 8.41 TLTVSQDD1014 pKa = 2.51 HH1015 pKa = 6.58 TYY1017 pKa = 11.3 AVDD1020 pKa = 3.51 SMTANTTGNVLTNDD1034 pKa = 3.5 IAPADD1039 pKa = 3.77 ALLTEE1044 pKa = 4.49 VNGVAISATGTTEE1057 pKa = 3.84 INGLYY1062 pKa = 8.62 GTLTIDD1068 pKa = 3.28 AKK1070 pKa = 11.3 GNYY1073 pKa = 8.46 TYY1075 pKa = 10.1 TLKK1078 pKa = 11.12 NGVGADD1084 pKa = 4.22 GIKK1087 pKa = 9.81 TPDD1090 pKa = 3.06 SFIYY1094 pKa = 9.0 TVKK1097 pKa = 10.74 APNGDD1102 pKa = 3.43 TDD1104 pKa = 3.94 TASLNITPTARR1115 pKa = 11.84 DD1116 pKa = 3.13 LDD1118 pKa = 4.77 AINDD1122 pKa = 3.64 VSDD1125 pKa = 3.84 TLSVATLQDD1134 pKa = 3.04 TAAWLDD1140 pKa = 3.64 SSVGSASWGLLGKK1153 pKa = 10.03 SGSGSGTFDD1162 pKa = 3.08 VATGTVLKK1170 pKa = 10.45 GASLVFDD1177 pKa = 4.06 VSTLITLGNLNISWTIQEE1195 pKa = 4.29 NGTVIRR1201 pKa = 11.84 NGTVPVANITLGGATVTVNLSGLEE1225 pKa = 4.01 LDD1227 pKa = 4.05 AGTYY1231 pKa = 7.75 TLNFTGTNTLAGAATITPRR1250 pKa = 11.84 VIGTTVDD1257 pKa = 3.36 LDD1259 pKa = 3.63 NFEE1262 pKa = 4.46 TSGTHH1267 pKa = 4.84 TVPGNIFDD1275 pKa = 4.44 GSDD1278 pKa = 2.76 AAGAMDD1284 pKa = 4.74 QLNTVNTRR1292 pKa = 11.84 LSISGYY1298 pKa = 9.16 NGSAATLDD1306 pKa = 3.51 AAANTTSATIQGHH1319 pKa = 5.86 YY1320 pKa = 8.63 GTLQINLDD1328 pKa = 3.33 GAYY1331 pKa = 9.35 TYY1333 pKa = 10.31 TLNNGVAISSITHH1346 pKa = 6.16 KK1347 pKa = 10.8 EE1348 pKa = 3.86 VFTYY1352 pKa = 10.71 QLDD1355 pKa = 4.04 DD1356 pKa = 4.3 KK1357 pKa = 11.44 NGHH1360 pKa = 5.78 TDD1362 pKa = 3.19 SATLTIDD1369 pKa = 3.2 MAPQIVSTNQNDD1381 pKa = 3.75 VLIGSAYY1388 pKa = 10.11 GDD1390 pKa = 3.38 TLIYY1394 pKa = 10.54 HH1395 pKa = 7.46 LLNSADD1401 pKa = 3.43 ATGGNGADD1409 pKa = 2.93 RR1410 pKa = 11.84 WQNFSTAQGDD1420 pKa = 3.76 KK1421 pKa = 10.55 IDD1423 pKa = 3.45 IHH1425 pKa = 6.44 EE1426 pKa = 5.34 LLTGWDD1432 pKa = 3.71 HH1433 pKa = 5.81 QTATLGNFIQVSTSGANTVISVDD1456 pKa = 3.37 RR1457 pKa = 11.84 DD1458 pKa = 3.52 GAGSAFKK1465 pKa = 10.2 ATDD1468 pKa = 3.49 LVTLEE1473 pKa = 4.08 NVQLSLNDD1481 pKa = 3.86 LLQNNHH1487 pKa = 7.48 LITGGG1492 pKa = 3.36
Molecular weight: 150.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.67
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.478
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.253
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.948
Patrickios 1.367
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A3S5D914|A0A3S5D914_SALBN Glutathione ABC transporter ATP-binding protein OS=Salmonella bongori OX=54736 GN=yheS_3 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6192
0
6192
1208264
29
2133
195.1
21.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.629 ± 0.037
1.254 ± 0.014
5.117 ± 0.029
5.395 ± 0.035
3.851 ± 0.023
7.439 ± 0.031
2.334 ± 0.018
5.833 ± 0.03
4.198 ± 0.03
10.545 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.889 ± 0.02
3.808 ± 0.025
4.569 ± 0.024
4.299 ± 0.028
5.991 ± 0.037
5.894 ± 0.026
5.536 ± 0.038
6.937 ± 0.03
1.579 ± 0.018
2.903 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here