Arthrobacter phage Isolde
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3M4I8|A0A3G3M4I8_9CAUD Uncharacterized protein OS=Arthrobacter phage Isolde OX=2419610 GN=15 PE=4 SV=1
MM1 pKa = 7.28 TKK3 pKa = 9.8 HH4 pKa = 5.54 YY5 pKa = 10.85 CPAQTFAGRR14 pKa = 11.84 YY15 pKa = 8.3 YY16 pKa = 10.91 VDD18 pKa = 4.31 PEE20 pKa = 3.93 PPEE23 pKa = 3.91 YY24 pKa = 10.78 CEE26 pKa = 5.37 AEE28 pKa = 4.23 VEE30 pKa = 4.43 EE31 pKa = 4.85 EE32 pKa = 4.49 GQFCPAHH39 pKa = 6.54 EE40 pKa = 4.58 EE41 pKa = 3.92 QDD43 pKa = 4.74 DD44 pKa = 4.01 YY45 pKa = 12.1 NPWGDD50 pKa = 3.43 EE51 pKa = 3.87
Molecular weight: 6.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.969
IPC2_protein 3.999
IPC_protein 3.846
Toseland 3.694
ProMoST 3.923
Dawson 3.808
Bjellqvist 4.05
Wikipedia 3.694
Rodwell 3.694
Grimsley 3.617
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.012
Thurlkill 3.732
EMBOSS 3.706
Sillero 3.961
Patrickios 0.006
IPC_peptide 3.783
IPC2_peptide 3.948
IPC2.peptide.svr19 3.962
Protein with the highest isoelectric point:
>tr|A0A3G3M3M8|A0A3G3M3M8_9CAUD Glutaredoxin OS=Arthrobacter phage Isolde OX=2419610 GN=71 PE=4 SV=1
MM1 pKa = 7.53 ASKK4 pKa = 10.23 PRR6 pKa = 11.84 WRR8 pKa = 11.84 LDD10 pKa = 3.04 HH11 pKa = 7.51 DD12 pKa = 4.05 PFARR16 pKa = 11.84 PLGCNGKK23 pKa = 9.54 YY24 pKa = 10.1 GVSGVQRR31 pKa = 11.84 HH32 pKa = 5.52 AADD35 pKa = 3.42 GTEE38 pKa = 4.11 TCKK41 pKa = 10.56 SCRR44 pKa = 11.84 EE45 pKa = 4.22 TYY47 pKa = 10.02 NHH49 pKa = 6.48 YY50 pKa = 10.43 KK51 pKa = 10.25 RR52 pKa = 11.84 EE53 pKa = 3.93 QRR55 pKa = 11.84 RR56 pKa = 11.84 GGIKK60 pKa = 9.27 PRR62 pKa = 11.84 KK63 pKa = 8.34 PKK65 pKa = 10.33 PCGTYY70 pKa = 10.3 AAVDD74 pKa = 3.15 RR75 pKa = 11.84 HH76 pKa = 4.79 QRR78 pKa = 11.84 RR79 pKa = 11.84 GEE81 pKa = 3.94 PLDD84 pKa = 3.51 FACRR88 pKa = 11.84 VARR91 pKa = 11.84 ARR93 pKa = 11.84 YY94 pKa = 6.62 QAEE97 pKa = 3.57 MRR99 pKa = 11.84 EE100 pKa = 4.1 RR101 pKa = 11.84 LEE103 pKa = 4.26 RR104 pKa = 11.84 EE105 pKa = 3.78 AVGNVHH111 pKa = 7.29 AEE113 pKa = 4.38 GKK115 pKa = 10.34 RR116 pKa = 11.84 LAEE119 pKa = 4.45 WIATRR124 pKa = 11.84 RR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 RR128 pKa = 11.84 CGRR131 pKa = 11.84 AGTHH135 pKa = 6.53 TIGHH139 pKa = 5.82 CEE141 pKa = 3.47 KK142 pKa = 10.41 CGVHH146 pKa = 4.73 RR147 pKa = 11.84 TPRR150 pKa = 11.84 RR151 pKa = 11.84 RR152 pKa = 11.84 AKK154 pKa = 9.84 EE155 pKa = 3.47 PAMNEE160 pKa = 4.24 QTDD163 pKa = 3.66 HH164 pKa = 6.4 AA165 pKa = 5.58
Molecular weight: 19.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.502
IPC_protein 10.233
Toseland 10.54
ProMoST 10.248
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.818
Grimsley 10.701
Solomon 10.76
Lehninger 10.73
Nozaki 10.584
DTASelect 10.365
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.526
IPC_peptide 10.774
IPC2_peptide 9.677
IPC2.peptide.svr19 8.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
96
0
96
16759
40
1261
174.6
19.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.791 ± 0.454
1.128 ± 0.177
6.468 ± 0.196
6.152 ± 0.285
2.763 ± 0.149
7.93 ± 0.302
2.452 ± 0.199
4.374 ± 0.162
4.511 ± 0.208
7.751 ± 0.306
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.105
3.353 ± 0.153
5.412 ± 0.308
3.562 ± 0.142
6.623 ± 0.307
5.376 ± 0.201
6.641 ± 0.258
6.91 ± 0.268
1.808 ± 0.137
2.727 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here