Arthrobacter phage Isolde

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M4I8|A0A3G3M4I8_9CAUD Uncharacterized protein OS=Arthrobacter phage Isolde OX=2419610 GN=15 PE=4 SV=1
MM1 pKa = 7.28TKK3 pKa = 9.8HH4 pKa = 5.54YY5 pKa = 10.85CPAQTFAGRR14 pKa = 11.84YY15 pKa = 8.3YY16 pKa = 10.91VDD18 pKa = 4.31PEE20 pKa = 3.93PPEE23 pKa = 3.91YY24 pKa = 10.78CEE26 pKa = 5.37AEE28 pKa = 4.23VEE30 pKa = 4.43EE31 pKa = 4.85EE32 pKa = 4.49GQFCPAHH39 pKa = 6.54EE40 pKa = 4.58EE41 pKa = 3.92QDD43 pKa = 4.74DD44 pKa = 4.01YY45 pKa = 12.1NPWGDD50 pKa = 3.43EE51 pKa = 3.87

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M3M8|A0A3G3M3M8_9CAUD Glutaredoxin OS=Arthrobacter phage Isolde OX=2419610 GN=71 PE=4 SV=1
MM1 pKa = 7.53ASKK4 pKa = 10.23PRR6 pKa = 11.84WRR8 pKa = 11.84LDD10 pKa = 3.04HH11 pKa = 7.51DD12 pKa = 4.05PFARR16 pKa = 11.84PLGCNGKK23 pKa = 9.54YY24 pKa = 10.1GVSGVQRR31 pKa = 11.84HH32 pKa = 5.52AADD35 pKa = 3.42GTEE38 pKa = 4.11TCKK41 pKa = 10.56SCRR44 pKa = 11.84EE45 pKa = 4.22TYY47 pKa = 10.02NHH49 pKa = 6.48YY50 pKa = 10.43KK51 pKa = 10.25RR52 pKa = 11.84EE53 pKa = 3.93QRR55 pKa = 11.84RR56 pKa = 11.84GGIKK60 pKa = 9.27PRR62 pKa = 11.84KK63 pKa = 8.34PKK65 pKa = 10.33PCGTYY70 pKa = 10.3AAVDD74 pKa = 3.15RR75 pKa = 11.84HH76 pKa = 4.79QRR78 pKa = 11.84RR79 pKa = 11.84GEE81 pKa = 3.94PLDD84 pKa = 3.51FACRR88 pKa = 11.84VARR91 pKa = 11.84ARR93 pKa = 11.84YY94 pKa = 6.62QAEE97 pKa = 3.57MRR99 pKa = 11.84EE100 pKa = 4.1RR101 pKa = 11.84LEE103 pKa = 4.26RR104 pKa = 11.84EE105 pKa = 3.78AVGNVHH111 pKa = 7.29AEE113 pKa = 4.38GKK115 pKa = 10.34RR116 pKa = 11.84LAEE119 pKa = 4.45WIATRR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84CGRR131 pKa = 11.84AGTHH135 pKa = 6.53TIGHH139 pKa = 5.82CEE141 pKa = 3.47KK142 pKa = 10.41CGVHH146 pKa = 4.73RR147 pKa = 11.84TPRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84AKK154 pKa = 9.84EE155 pKa = 3.47PAMNEE160 pKa = 4.24QTDD163 pKa = 3.66HH164 pKa = 6.4AA165 pKa = 5.58

Molecular weight: 19.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16759

40

1261

174.6

19.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.791 ± 0.454

1.128 ± 0.177

6.468 ± 0.196

6.152 ± 0.285

2.763 ± 0.149

7.93 ± 0.302

2.452 ± 0.199

4.374 ± 0.162

4.511 ± 0.208

7.751 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.105

3.353 ± 0.153

5.412 ± 0.308

3.562 ± 0.142

6.623 ± 0.307

5.376 ± 0.201

6.641 ± 0.258

6.91 ± 0.268

1.808 ± 0.137

2.727 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski