Amino acid dipepetide frequency for Arthrobacter phage Isolde

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.842AlaAla: 17.842 ± 1.702
0.835AlaCys: 0.835 ± 0.265
7.757AlaAsp: 7.757 ± 0.852
9.548AlaGlu: 9.548 ± 0.793
2.447AlaPhe: 2.447 ± 0.35
10.443AlaGly: 10.443 ± 1.184
2.387AlaHis: 2.387 ± 0.453
5.251AlaIle: 5.251 ± 0.531
5.43AlaLys: 5.43 ± 0.684
9.249AlaLeu: 9.249 ± 0.911
2.924AlaMet: 2.924 ± 0.467
4.237AlaAsn: 4.237 ± 0.642
4.475AlaPro: 4.475 ± 0.671
4.595AlaGln: 4.595 ± 0.5
6.385AlaArg: 6.385 ± 0.819
5.729AlaSer: 5.729 ± 0.71
5.49AlaThr: 5.49 ± 0.428
8.056AlaVal: 8.056 ± 0.589
2.268AlaTrp: 2.268 ± 0.411
2.566AlaTyr: 2.566 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
0.537CysAla: 0.537 ± 0.191
0.179CysCys: 0.179 ± 0.121
1.372CysAsp: 1.372 ± 0.356
0.955CysGlu: 0.955 ± 0.338
0.179CysPhe: 0.179 ± 0.112
1.253CysGly: 1.253 ± 0.359
0.179CysHis: 0.179 ± 0.096
0.298CysIle: 0.298 ± 0.112
0.776CysLys: 0.776 ± 0.208
0.477CysLeu: 0.477 ± 0.19
0.119CysMet: 0.119 ± 0.077
0.358CysAsn: 0.358 ± 0.148
1.372CysPro: 1.372 ± 0.301
0.239CysGln: 0.239 ± 0.117
0.477CysArg: 0.477 ± 0.209
0.597CysSer: 0.597 ± 0.208
0.955CysThr: 0.955 ± 0.233
0.418CysVal: 0.418 ± 0.162
0.239CysTrp: 0.239 ± 0.126
0.298CysTyr: 0.298 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
7.22AspAla: 7.22 ± 0.596
0.537AspCys: 0.537 ± 0.196
3.879AspAsp: 3.879 ± 0.599
4.058AspGlu: 4.058 ± 0.613
2.148AspPhe: 2.148 ± 0.397
7.459AspGly: 7.459 ± 0.847
1.85AspHis: 1.85 ± 0.406
2.208AspIle: 2.208 ± 0.378
2.387AspLys: 2.387 ± 0.398
5.848AspLeu: 5.848 ± 0.577
1.372AspMet: 1.372 ± 0.248
1.611AspAsn: 1.611 ± 0.328
4.356AspPro: 4.356 ± 0.633
1.372AspGln: 1.372 ± 0.267
4.058AspArg: 4.058 ± 0.51
3.342AspSer: 3.342 ± 0.616
2.805AspThr: 2.805 ± 0.438
4.834AspVal: 4.834 ± 0.543
1.731AspTrp: 1.731 ± 0.325
1.611AspTyr: 1.611 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
7.937GluAla: 7.937 ± 0.714
0.418GluCys: 0.418 ± 0.163
3.103GluAsp: 3.103 ± 0.457
2.566GluGlu: 2.566 ± 0.411
1.79GluPhe: 1.79 ± 0.256
3.879GluGly: 3.879 ± 0.476
1.969GluHis: 1.969 ± 0.399
2.447GluIle: 2.447 ± 0.423
2.805GluLys: 2.805 ± 0.409
4.834GluLeu: 4.834 ± 0.569
1.79GluMet: 1.79 ± 0.313
2.148GluAsn: 2.148 ± 0.425
2.805GluPro: 2.805 ± 0.481
3.282GluGln: 3.282 ± 0.466
4.535GluArg: 4.535 ± 0.613
3.342GluSer: 3.342 ± 0.531
3.819GluThr: 3.819 ± 0.456
4.774GluVal: 4.774 ± 0.517
1.671GluTrp: 1.671 ± 0.34
1.611GluTyr: 1.611 ± 0.284
0.0GluXaa: 0.0 ± 0.0
Phe
3.043PheAla: 3.043 ± 0.536
0.597PheCys: 0.597 ± 0.223
2.745PheAsp: 2.745 ± 0.412
1.551PheGlu: 1.551 ± 0.333
0.656PhePhe: 0.656 ± 0.2
2.387PheGly: 2.387 ± 0.397
1.134PheHis: 1.134 ± 0.257
0.835PheIle: 0.835 ± 0.197
0.955PheLys: 0.955 ± 0.234
1.731PheLeu: 1.731 ± 0.345
0.597PheMet: 0.597 ± 0.202
0.656PheAsn: 0.656 ± 0.176
1.253PhePro: 1.253 ± 0.258
1.014PheGln: 1.014 ± 0.247
1.969PheArg: 1.969 ± 0.332
1.313PheSer: 1.313 ± 0.268
2.208PheThr: 2.208 ± 0.35
1.551PheVal: 1.551 ± 0.308
0.537PheTrp: 0.537 ± 0.167
0.895PheTyr: 0.895 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
6.922GlyAla: 6.922 ± 0.906
0.955GlyCys: 0.955 ± 0.224
4.893GlyAsp: 4.893 ± 0.492
4.595GlyGlu: 4.595 ± 0.521
2.864GlyPhe: 2.864 ± 0.374
6.266GlyGly: 6.266 ± 0.698
1.731GlyHis: 1.731 ± 0.31
3.222GlyIle: 3.222 ± 0.624
4.416GlyLys: 4.416 ± 0.486
5.908GlyLeu: 5.908 ± 0.741
1.91GlyMet: 1.91 ± 0.315
2.864GlyAsn: 2.864 ± 0.494
3.938GlyPro: 3.938 ± 0.655
2.447GlyGln: 2.447 ± 0.282
5.49GlyArg: 5.49 ± 0.503
4.595GlySer: 4.595 ± 0.539
6.027GlyThr: 6.027 ± 0.727
5.49GlyVal: 5.49 ± 0.683
2.148GlyTrp: 2.148 ± 0.407
2.626GlyTyr: 2.626 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
2.447HisAla: 2.447 ± 0.429
0.358HisCys: 0.358 ± 0.14
1.551HisAsp: 1.551 ± 0.282
1.91HisGlu: 1.91 ± 0.442
0.656HisPhe: 0.656 ± 0.265
1.611HisGly: 1.611 ± 0.314
0.895HisHis: 0.895 ± 0.297
1.253HisIle: 1.253 ± 0.28
0.835HisLys: 0.835 ± 0.234
2.387HisLeu: 2.387 ± 0.398
0.537HisMet: 0.537 ± 0.187
1.014HisAsn: 1.014 ± 0.265
1.313HisPro: 1.313 ± 0.279
0.955HisGln: 0.955 ± 0.22
1.134HisArg: 1.134 ± 0.274
1.074HisSer: 1.074 ± 0.292
1.432HisThr: 1.432 ± 0.269
1.79HisVal: 1.79 ± 0.273
0.298HisTrp: 0.298 ± 0.136
1.074HisTyr: 1.074 ± 0.269
0.0HisXaa: 0.0 ± 0.0
Ile
5.013IleAla: 5.013 ± 0.563
0.418IleCys: 0.418 ± 0.143
3.461IleAsp: 3.461 ± 0.491
3.282IleGlu: 3.282 ± 0.434
1.014IlePhe: 1.014 ± 0.235
2.864IleGly: 2.864 ± 0.469
1.014IleHis: 1.014 ± 0.22
1.372IleIle: 1.372 ± 0.269
1.79IleLys: 1.79 ± 0.287
2.984IleLeu: 2.984 ± 0.365
0.776IleMet: 0.776 ± 0.192
1.193IleAsn: 1.193 ± 0.258
2.924IlePro: 2.924 ± 0.461
1.313IleGln: 1.313 ± 0.245
3.043IleArg: 3.043 ± 0.41
2.447IleSer: 2.447 ± 0.347
4.356IleThr: 4.356 ± 0.524
3.282IleVal: 3.282 ± 0.471
0.119IleTrp: 0.119 ± 0.088
1.074IleTyr: 1.074 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
5.967LysAla: 5.967 ± 0.696
0.597LysCys: 0.597 ± 0.233
2.089LysAsp: 2.089 ± 0.326
2.327LysGlu: 2.327 ± 0.396
1.372LysPhe: 1.372 ± 0.276
2.685LysGly: 2.685 ± 0.433
1.134LysHis: 1.134 ± 0.308
1.85LysIle: 1.85 ± 0.387
1.731LysLys: 1.731 ± 0.242
3.222LysLeu: 3.222 ± 0.445
1.611LysMet: 1.611 ± 0.255
2.148LysAsn: 2.148 ± 0.321
3.64LysPro: 3.64 ± 0.517
1.671LysGln: 1.671 ± 0.334
3.222LysArg: 3.222 ± 0.522
2.148LysSer: 2.148 ± 0.382
2.984LysThr: 2.984 ± 0.359
2.805LysVal: 2.805 ± 0.44
0.776LysTrp: 0.776 ± 0.229
1.134LysTyr: 1.134 ± 0.282
0.0LysXaa: 0.0 ± 0.0
Leu
9.667LeuAla: 9.667 ± 0.932
0.776LeuCys: 0.776 ± 0.218
5.609LeuAsp: 5.609 ± 0.648
3.998LeuGlu: 3.998 ± 0.545
1.79LeuPhe: 1.79 ± 0.377
6.027LeuGly: 6.027 ± 0.599
1.492LeuHis: 1.492 ± 0.423
3.998LeuIle: 3.998 ± 0.446
3.759LeuLys: 3.759 ± 0.492
5.788LeuLeu: 5.788 ± 0.624
1.432LeuMet: 1.432 ± 0.282
2.566LeuAsn: 2.566 ± 0.34
5.192LeuPro: 5.192 ± 0.581
2.626LeuGln: 2.626 ± 0.386
5.788LeuArg: 5.788 ± 0.678
3.938LeuSer: 3.938 ± 0.597
6.146LeuThr: 6.146 ± 0.575
4.237LeuVal: 4.237 ± 0.554
1.134LeuTrp: 1.134 ± 0.284
1.551LeuTyr: 1.551 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
3.282MetAla: 3.282 ± 0.423
0.119MetCys: 0.119 ± 0.084
1.372MetAsp: 1.372 ± 0.324
1.014MetGlu: 1.014 ± 0.233
0.716MetPhe: 0.716 ± 0.191
1.134MetGly: 1.134 ± 0.293
0.537MetHis: 0.537 ± 0.16
0.656MetIle: 0.656 ± 0.201
1.074MetLys: 1.074 ± 0.233
2.089MetLeu: 2.089 ± 0.325
0.418MetMet: 0.418 ± 0.134
0.895MetAsn: 0.895 ± 0.252
1.432MetPro: 1.432 ± 0.309
0.716MetGln: 0.716 ± 0.194
1.193MetArg: 1.193 ± 0.334
1.731MetSer: 1.731 ± 0.281
2.327MetThr: 2.327 ± 0.398
1.193MetVal: 1.193 ± 0.25
0.239MetTrp: 0.239 ± 0.112
0.537MetTyr: 0.537 ± 0.17
0.0MetXaa: 0.0 ± 0.0
Asn
4.774AsnAla: 4.774 ± 0.536
0.239AsnCys: 0.239 ± 0.119
1.79AsnAsp: 1.79 ± 0.361
1.79AsnGlu: 1.79 ± 0.431
0.656AsnPhe: 0.656 ± 0.189
3.64AsnGly: 3.64 ± 0.493
1.492AsnHis: 1.492 ± 0.334
0.776AsnIle: 0.776 ± 0.182
0.895AsnLys: 0.895 ± 0.186
2.506AsnLeu: 2.506 ± 0.402
0.597AsnMet: 0.597 ± 0.172
0.776AsnAsn: 0.776 ± 0.253
2.327AsnPro: 2.327 ± 0.417
1.432AsnGln: 1.432 ± 0.344
2.268AsnArg: 2.268 ± 0.373
1.85AsnSer: 1.85 ± 0.389
1.85AsnThr: 1.85 ± 0.367
2.626AsnVal: 2.626 ± 0.397
0.656AsnTrp: 0.656 ± 0.215
0.597AsnTyr: 0.597 ± 0.194
0.0AsnXaa: 0.0 ± 0.0
Pro
5.371ProAla: 5.371 ± 0.714
0.835ProCys: 0.835 ± 0.213
3.938ProAsp: 3.938 ± 0.549
3.998ProGlu: 3.998 ± 0.671
1.969ProPhe: 1.969 ± 0.276
4.058ProGly: 4.058 ± 0.462
1.014ProHis: 1.014 ± 0.195
2.148ProIle: 2.148 ± 0.339
2.924ProLys: 2.924 ± 0.467
3.998ProLeu: 3.998 ± 0.594
1.014ProMet: 1.014 ± 0.276
1.671ProAsn: 1.671 ± 0.349
3.342ProPro: 3.342 ± 0.569
1.79ProGln: 1.79 ± 0.371
3.401ProArg: 3.401 ± 0.494
3.103ProSer: 3.103 ± 0.47
3.103ProThr: 3.103 ± 0.381
4.475ProVal: 4.475 ± 0.631
0.895ProTrp: 0.895 ± 0.215
1.014ProTyr: 1.014 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
4.475GlnAla: 4.475 ± 0.555
0.358GlnCys: 0.358 ± 0.141
1.91GlnAsp: 1.91 ± 0.328
1.313GlnGlu: 1.313 ± 0.36
0.955GlnPhe: 0.955 ± 0.204
3.282GlnGly: 3.282 ± 0.489
0.477GlnHis: 0.477 ± 0.17
1.611GlnIle: 1.611 ± 0.262
1.492GlnLys: 1.492 ± 0.372
3.401GlnLeu: 3.401 ± 0.458
0.835GlnMet: 0.835 ± 0.202
0.895GlnAsn: 0.895 ± 0.195
1.432GlnPro: 1.432 ± 0.37
1.731GlnGln: 1.731 ± 0.287
2.148GlnArg: 2.148 ± 0.416
2.626GlnSer: 2.626 ± 0.385
2.685GlnThr: 2.685 ± 0.514
2.447GlnVal: 2.447 ± 0.394
0.298GlnTrp: 0.298 ± 0.139
1.253GlnTyr: 1.253 ± 0.314
0.0GlnXaa: 0.0 ± 0.0
Arg
6.206ArgAla: 6.206 ± 0.533
1.134ArgCys: 1.134 ± 0.287
3.998ArgAsp: 3.998 ± 0.595
4.416ArgGlu: 4.416 ± 0.563
1.79ArgPhe: 1.79 ± 0.343
4.714ArgGly: 4.714 ± 0.465
2.208ArgHis: 2.208 ± 0.575
4.535ArgIle: 4.535 ± 0.485
2.745ArgLys: 2.745 ± 0.362
5.967ArgLeu: 5.967 ± 0.567
1.611ArgMet: 1.611 ± 0.384
1.969ArgAsn: 1.969 ± 0.351
2.626ArgPro: 2.626 ± 0.391
1.731ArgGln: 1.731 ± 0.363
5.251ArgArg: 5.251 ± 0.757
3.58ArgSer: 3.58 ± 0.476
3.64ArgThr: 3.64 ± 0.439
4.117ArgVal: 4.117 ± 0.65
1.85ArgTrp: 1.85 ± 0.369
2.148ArgTyr: 2.148 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
5.729SerAla: 5.729 ± 0.735
0.537SerCys: 0.537 ± 0.183
3.521SerAsp: 3.521 ± 0.427
3.043SerGlu: 3.043 ± 0.429
1.551SerPhe: 1.551 ± 0.286
4.834SerGly: 4.834 ± 0.593
0.776SerHis: 0.776 ± 0.236
3.879SerIle: 3.879 ± 0.399
2.566SerLys: 2.566 ± 0.335
4.177SerLeu: 4.177 ± 0.577
1.492SerMet: 1.492 ± 0.268
1.551SerAsn: 1.551 ± 0.301
2.148SerPro: 2.148 ± 0.361
1.969SerGln: 1.969 ± 0.335
3.64SerArg: 3.64 ± 0.39
2.387SerSer: 2.387 ± 0.406
3.64SerThr: 3.64 ± 0.386
3.521SerVal: 3.521 ± 0.566
0.776SerTrp: 0.776 ± 0.189
2.029SerTyr: 2.029 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
8.414ThrAla: 8.414 ± 0.74
0.656ThrCys: 0.656 ± 0.216
3.819ThrAsp: 3.819 ± 0.418
4.058ThrGlu: 4.058 ± 0.441
2.208ThrPhe: 2.208 ± 0.326
5.55ThrGly: 5.55 ± 0.688
1.85ThrHis: 1.85 ± 0.304
2.924ThrIle: 2.924 ± 0.388
2.566ThrLys: 2.566 ± 0.349
4.714ThrLeu: 4.714 ± 0.556
1.253ThrMet: 1.253 ± 0.317
2.506ThrAsn: 2.506 ± 0.393
4.296ThrPro: 4.296 ± 0.51
2.089ThrGln: 2.089 ± 0.313
3.222ThrArg: 3.222 ± 0.409
3.282ThrSer: 3.282 ± 0.431
4.296ThrThr: 4.296 ± 0.516
5.55ThrVal: 5.55 ± 0.758
0.716ThrTrp: 0.716 ± 0.257
2.447ThrTyr: 2.447 ± 0.367
0.0ThrXaa: 0.0 ± 0.0
Val
7.996ValAla: 7.996 ± 0.872
0.597ValCys: 0.597 ± 0.159
4.654ValAsp: 4.654 ± 0.652
4.416ValGlu: 4.416 ± 0.595
1.611ValPhe: 1.611 ± 0.294
4.237ValGly: 4.237 ± 0.484
1.551ValHis: 1.551 ± 0.334
2.805ValIle: 2.805 ± 0.5
3.938ValLys: 3.938 ± 0.498
4.834ValLeu: 4.834 ± 0.534
1.611ValMet: 1.611 ± 0.333
2.506ValAsn: 2.506 ± 0.346
3.521ValPro: 3.521 ± 0.429
2.864ValGln: 2.864 ± 0.363
4.953ValArg: 4.953 ± 0.552
4.237ValSer: 4.237 ± 0.542
5.251ValThr: 5.251 ± 0.552
4.714ValVal: 4.714 ± 0.55
0.895ValTrp: 0.895 ± 0.218
1.91ValTyr: 1.91 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
2.745TrpAla: 2.745 ± 0.429
0.418TrpCys: 0.418 ± 0.148
1.85TrpAsp: 1.85 ± 0.39
0.656TrpGlu: 0.656 ± 0.239
0.358TrpPhe: 0.358 ± 0.127
0.955TrpGly: 0.955 ± 0.211
0.418TrpHis: 0.418 ± 0.193
0.835TrpIle: 0.835 ± 0.233
0.776TrpLys: 0.776 ± 0.202
1.432TrpLeu: 1.432 ± 0.289
0.06TrpMet: 0.06 ± 0.051
0.835TrpAsn: 0.835 ± 0.261
0.537TrpPro: 0.537 ± 0.224
0.597TrpGln: 0.597 ± 0.18
1.79TrpArg: 1.79 ± 0.334
0.955TrpSer: 0.955 ± 0.213
0.955TrpThr: 0.955 ± 0.231
1.074TrpVal: 1.074 ± 0.263
0.537TrpTrp: 0.537 ± 0.182
0.298TrpTyr: 0.298 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.327TyrAla: 2.327 ± 0.369
0.716TyrCys: 0.716 ± 0.239
1.372TyrAsp: 1.372 ± 0.279
2.029TyrGlu: 2.029 ± 0.349
0.895TyrPhe: 0.895 ± 0.19
2.029TyrGly: 2.029 ± 0.318
0.418TyrHis: 0.418 ± 0.142
0.835TyrIle: 0.835 ± 0.206
1.253TyrLys: 1.253 ± 0.278
1.969TyrLeu: 1.969 ± 0.359
0.656TyrMet: 0.656 ± 0.177
1.134TyrAsn: 1.134 ± 0.262
1.193TyrPro: 1.193 ± 0.259
1.193TyrGln: 1.193 ± 0.242
2.268TyrArg: 2.268 ± 0.347
1.492TyrSer: 1.492 ± 0.288
2.447TyrThr: 2.447 ± 0.384
2.148TyrVal: 2.148 ± 0.299
0.298TyrTrp: 0.298 ± 0.181
0.597TyrTyr: 0.597 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (16759 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski