Peronospora effusa

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Peronospora

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M6VER7|A0A3M6VER7_9STRA Foie-gras_1 domain-containing protein OS=Peronospora effusa OX=542832 GN=DD237_003503 PE=4 SV=1
MM1 pKa = 6.99NVAFRR6 pKa = 11.84FYY8 pKa = 11.32ALLYY12 pKa = 9.33LVSMKK17 pKa = 11.05ANGLVVQPQTLPADD31 pKa = 4.59SIQPQIQRR39 pKa = 11.84NEE41 pKa = 4.23SEE43 pKa = 4.38SDD45 pKa = 3.15AATDD49 pKa = 4.53FLDD52 pKa = 4.02EE53 pKa = 3.94LAAAIFKK60 pKa = 10.36RR61 pKa = 11.84YY62 pKa = 6.41PTNDD66 pKa = 2.95QSNFAAFIDD75 pKa = 3.8ALLDD79 pKa = 3.98LGSTSNEE86 pKa = 3.91STIHH90 pKa = 6.2SNGSSTYY97 pKa = 9.62DD98 pKa = 3.08HH99 pKa = 7.08SDD101 pKa = 3.37VYY103 pKa = 11.49VGDD106 pKa = 3.81ASEE109 pKa = 4.48EE110 pKa = 4.21EE111 pKa = 4.41EE112 pKa = 4.88GDD114 pKa = 3.69DD115 pKa = 4.89DD116 pKa = 4.21VGHH119 pKa = 6.47VFTPFPEE126 pKa = 4.47LLSMNADD133 pKa = 3.66TSASSTYY140 pKa = 7.41EE141 pKa = 3.81TT142 pKa = 4.46

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M6VUQ2|A0A3M6VUQ2_9STRA L-type lectin-like domain-containing protein OS=Peronospora effusa OX=542832 GN=DD238_000598 PE=4 SV=1
MM1 pKa = 7.37IRR3 pKa = 11.84TLRR6 pKa = 11.84VISRR10 pKa = 11.84PLVWTLKK17 pKa = 10.52SVLLSSTARR26 pKa = 11.84PSSFRR31 pKa = 11.84FGTQLARR38 pKa = 11.84NVSVRR43 pKa = 11.84SLAVTTAVPTVLSS56 pKa = 3.82

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8522

0

8522

4034935

32

6132

473.5

52.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.089 ± 0.027

1.688 ± 0.011

5.711 ± 0.017

6.681 ± 0.027

3.841 ± 0.016

5.791 ± 0.024

2.456 ± 0.011

4.487 ± 0.018

5.692 ± 0.026

9.57 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.011

3.805 ± 0.011

4.291 ± 0.018

4.232 ± 0.019

5.948 ± 0.02

8.205 ± 0.03

5.854 ± 0.017

7.196 ± 0.021

1.129 ± 0.008

2.639 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski