Nocardia ignorata

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6P533|A0A4R6P533_9NOCA LGFP repeat-containing protein OS=Nocardia ignorata OX=145285 GN=DFR75_105166 PE=4 SV=1
MM1 pKa = 7.66NDD3 pKa = 3.14YY4 pKa = 11.37KK5 pKa = 11.02LFQCVQCGFEE15 pKa = 4.07YY16 pKa = 10.91DD17 pKa = 3.92EE18 pKa = 4.49ALGWPEE24 pKa = 5.14DD25 pKa = 4.18GIEE28 pKa = 4.69PGTRR32 pKa = 11.84WDD34 pKa = 5.76DD35 pKa = 4.2IPDD38 pKa = 3.72DD39 pKa = 4.45WSCPDD44 pKa = 3.97CGAAKK49 pKa = 10.43ADD51 pKa = 3.3FDD53 pKa = 3.73MRR55 pKa = 11.84EE56 pKa = 3.89VSRR59 pKa = 11.84GG60 pKa = 3.34

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6PXC5|A0A4R6PXC5_9NOCA 3-phosphoshikimate 1-carboxyvinyltransferase OS=Nocardia ignorata OX=145285 GN=aroA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6616

0

6616

2102982

26

14630

317.9

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.385 ± 0.05

0.757 ± 0.01

6.232 ± 0.03

5.448 ± 0.026

2.96 ± 0.018

8.781 ± 0.029

2.213 ± 0.016

4.127 ± 0.021

1.987 ± 0.025

10.155 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.904 ± 0.014

2.005 ± 0.016

5.851 ± 0.034

2.822 ± 0.017

7.53 ± 0.034

5.2 ± 0.019

6.451 ± 0.024

8.702 ± 0.034

1.453 ± 0.013

2.036 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski