Sheep polyomavirus 1
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3UUJ6|A0A0E3UUJ6_9POLY ALTO OS=Sheep polyomavirus 1 OX=1634381 PE=4 SV=1
MM1 pKa = 7.22 NKK3 pKa = 9.26 YY4 pKa = 10.14 RR5 pKa = 11.84 KK6 pKa = 9.84 LFWEE10 pKa = 5.77 GILQLVTKK18 pKa = 10.63 ASKK21 pKa = 10.61 DD22 pKa = 3.43 QLNCLLSLWLGALEE36 pKa = 4.42 CLEE39 pKa = 4.12 EE40 pKa = 5.51 AIKK43 pKa = 10.95 LKK45 pKa = 10.84 LKK47 pKa = 10.26 EE48 pKa = 4.49 LEE50 pKa = 4.38 KK51 pKa = 9.99 QTQDD55 pKa = 3.26 TVDD58 pKa = 3.76 GLDD61 pKa = 3.65 GPVGVSQVLTCSQTLEE77 pKa = 4.24 SCKK80 pKa = 9.2 QTLAQCPNWSAGGATVEE97 pKa = 4.31 EE98 pKa = 4.29 TDD100 pKa = 3.81 LAAEE104 pKa = 4.14 QTVPAGVSVPDD115 pKa = 3.95 GLPTPVLRR123 pKa = 11.84 AVYY126 pKa = 9.28 FALRR130 pKa = 11.84 VLEE133 pKa = 4.2 EE134 pKa = 4.04 RR135 pKa = 11.84 ASEE138 pKa = 4.48 DD139 pKa = 4.0 GEE141 pKa = 4.3 QEE143 pKa = 3.84 PSDD146 pKa = 3.92 AEE148 pKa = 4.03 EE149 pKa = 3.95 EE150 pKa = 4.21 LRR152 pKa = 11.84 DD153 pKa = 4.52 LEE155 pKa = 4.34 RR156 pKa = 11.84 ALQSGHH162 pKa = 6.17 SSSII166 pKa = 3.43
Molecular weight: 18.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.539
IPC2_protein 4.482
IPC_protein 4.38
Toseland 4.228
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.151
Rodwell 4.215
Grimsley 4.139
Solomon 4.304
Lehninger 4.253
Nozaki 4.418
DTASelect 4.533
Thurlkill 4.228
EMBOSS 4.177
Sillero 4.482
Patrickios 3.859
IPC_peptide 4.317
IPC2_peptide 4.469
IPC2.peptide.svr19 4.436
Protein with the highest isoelectric point:
>tr|A0A0E3UVH1|A0A0E3UVH1_9POLY Large T antigen OS=Sheep polyomavirus 1 OX=1634381 PE=4 SV=1
MM1 pKa = 7.41 LNSVSYY7 pKa = 9.84 RR8 pKa = 11.84 YY9 pKa = 10.33 HH10 pKa = 8.32 LMVPRR15 pKa = 11.84 SGRR18 pKa = 11.84 AGGRR22 pKa = 11.84 HH23 pKa = 5.47 SMLISSAMKK32 pKa = 9.09 RR33 pKa = 11.84 WRR35 pKa = 11.84 RR36 pKa = 11.84 NPLPNSHH43 pKa = 6.28 GEE45 pKa = 4.33 KK46 pKa = 8.71 KK47 pKa = 7.08 THH49 pKa = 6.78 RR50 pKa = 11.84 APSLHH55 pKa = 6.92 RR56 pKa = 11.84 PRR58 pKa = 11.84 IRR60 pKa = 11.84 HH61 pKa = 5.92 KK62 pKa = 10.16 GTLRR66 pKa = 11.84 TCLLKK71 pKa = 10.61 FKK73 pKa = 10.2 RR74 pKa = 11.84 CSVKK78 pKa = 10.45 LFSAIKK84 pKa = 10.19 LL85 pKa = 3.77
Molecular weight: 9.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.21
IPC2_protein 10.599
IPC_protein 11.93
Toseland 12.091
ProMoST 12.574
Dawson 12.091
Bjellqvist 12.076
Wikipedia 12.559
Rodwell 11.901
Grimsley 12.135
Solomon 12.574
Lehninger 12.486
Nozaki 12.091
DTASelect 12.076
Thurlkill 12.091
EMBOSS 12.588
Sillero 12.091
Patrickios 11.623
IPC_peptide 12.574
IPC2_peptide 11.564
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2027
85
664
289.6
32.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.499 ± 1.43
1.727 ± 0.482
4.588 ± 0.344
6.561 ± 0.689
3.651 ± 0.619
6.167 ± 0.805
2.269 ± 0.304
3.7 ± 0.382
4.785 ± 0.896
10.163 ± 0.831
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.157 ± 0.407
4.193 ± 0.632
6.709 ± 0.497
5.032 ± 0.533
6.315 ± 0.882
6.512 ± 0.535
5.673 ± 0.55
5.821 ± 0.709
1.727 ± 0.311
3.749 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here