Mesorhizobium sp. YM1C-6-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3F2U5A1|A0A3F2U5A1_9RHIZ YeeE/YedE family protein OS=Mesorhizobium sp. YM1C-6-2 OX=1827501 GN=D8676_16700 PE=4 SV=1
MM1 pKa = 7.51SGYY4 pKa = 11.01ALAADD9 pKa = 3.55AVVEE13 pKa = 4.15EE14 pKa = 4.67VVVDD18 pKa = 3.89VAPVFIWTGGYY29 pKa = 9.89VGLHH33 pKa = 6.32GGWAWGQEE41 pKa = 3.88NDD43 pKa = 3.48NLSVFEE49 pKa = 4.59EE50 pKa = 4.54PPPTEE55 pKa = 4.09EE56 pKa = 4.62QPVADD61 pKa = 4.7EE62 pKa = 4.38FDD64 pKa = 3.27VDD66 pKa = 4.0GFIGGIHH73 pKa = 7.09AGYY76 pKa = 9.45NWQSGSFVYY85 pKa = 10.6GLEE88 pKa = 3.82GDD90 pKa = 3.72IDD92 pKa = 3.98YY93 pKa = 11.6ADD95 pKa = 3.74INGDD99 pKa = 3.34ADD101 pKa = 3.93FGGGTGNLSLDD112 pKa = 3.95SDD114 pKa = 3.95WQGSLRR120 pKa = 11.84LRR122 pKa = 11.84AGYY125 pKa = 11.24AMDD128 pKa = 3.24TWLLYY133 pKa = 9.71ATGGVAFGHH142 pKa = 6.94AEE144 pKa = 4.05LEE146 pKa = 4.14ASNLGVRR153 pKa = 11.84EE154 pKa = 3.99SDD156 pKa = 3.59EE157 pKa = 4.11NTHH160 pKa = 5.96VGWTIGGGVEE170 pKa = 5.25KK171 pKa = 10.65AFTPNWIGRR180 pKa = 11.84FEE182 pKa = 3.96VRR184 pKa = 11.84YY185 pKa = 8.36TDD187 pKa = 5.35FGSEE191 pKa = 4.26DD192 pKa = 3.87YY193 pKa = 11.4DD194 pKa = 4.62LGTLGDD200 pKa = 3.92SVEE203 pKa = 4.54ADD205 pKa = 2.88WHH207 pKa = 4.8QTAVLLGVSYY217 pKa = 11.16KK218 pKa = 10.45FF219 pKa = 3.61

Molecular weight: 23.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3F2U4L1|A0A3F2U4L1_9RHIZ Disulfide bond formation protein B OS=Mesorhizobium sp. YM1C-6-2 OX=1827501 GN=D8676_17995 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5230

0

5230

1606736

29

2858

307.2

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.438 ± 0.046

0.767 ± 0.01

5.717 ± 0.027

5.958 ± 0.03

3.89 ± 0.021

8.772 ± 0.032

1.934 ± 0.017

5.341 ± 0.023

3.607 ± 0.028

9.794 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.016

2.612 ± 0.018

5.059 ± 0.026

2.889 ± 0.019

6.897 ± 0.033

5.459 ± 0.02

5.176 ± 0.024

7.597 ± 0.026

1.325 ± 0.014

2.265 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski