Tortoise microvirus 20

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W9B4|A0A4P8W9B4_9VIRU Uncharacterized protein OS=Tortoise microvirus 20 OX=2583122 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.58IKK4 pKa = 10.18IADD7 pKa = 4.0IIEE10 pKa = 4.22ILQEE14 pKa = 4.33AIEE17 pKa = 4.47SKK19 pKa = 10.68RR20 pKa = 11.84LTNLEE25 pKa = 3.6IEE27 pKa = 4.53QYY29 pKa = 10.35RR30 pKa = 11.84EE31 pKa = 3.68QLEE34 pKa = 4.33EE35 pKa = 5.37IIDD38 pKa = 3.58NMMIQTEE45 pKa = 4.14NTYY48 pKa = 9.22TCTCTICDD56 pKa = 3.57EE57 pKa = 4.46CKK59 pKa = 10.82EE60 pKa = 4.33EE61 pKa = 3.92IKK63 pKa = 10.4QAYY66 pKa = 7.58EE67 pKa = 3.93CEE69 pKa = 4.11HH70 pKa = 5.73FRR72 pKa = 11.84EE73 pKa = 4.36VIKK76 pKa = 10.71RR77 pKa = 11.84INGYY81 pKa = 9.87KK82 pKa = 9.84EE83 pKa = 4.51DD84 pKa = 5.37LINEE88 pKa = 3.89QDD90 pKa = 3.14

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W5W3|A0A4P8W5W3_9VIRU Replication initiation protein OS=Tortoise microvirus 20 OX=2583122 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.59SNARR6 pKa = 11.84KK7 pKa = 9.48RR8 pKa = 11.84RR9 pKa = 11.84ANKK12 pKa = 9.95GGAQMEE18 pKa = 4.38QATIQGDD25 pKa = 3.12IGTGGNSNAMATMEE39 pKa = 4.24ATNTNARR46 pKa = 11.84PRR48 pKa = 11.84PGVDD52 pKa = 2.65EE53 pKa = 4.7SGLFRR58 pKa = 11.84RR59 pKa = 11.84IGRR62 pKa = 11.84FLGTGHH68 pKa = 6.17WHH70 pKa = 7.38DD71 pKa = 4.32PNIAIDD77 pKa = 3.44NEE79 pKa = 4.01MRR81 pKa = 11.84QRR83 pKa = 11.84EE84 pKa = 3.99WDD86 pKa = 3.31EE87 pKa = 3.66RR88 pKa = 11.84TSYY91 pKa = 8.93QRR93 pKa = 11.84RR94 pKa = 11.84MEE96 pKa = 4.21AMRR99 pKa = 11.84TAGLNPMLMLQGGGGGGGMSGASTNTEE126 pKa = 4.01EE127 pKa = 5.02GEE129 pKa = 4.42SMPNLLPLLLVALTLGKK146 pKa = 10.11SLPAKK151 pKa = 9.21GAKK154 pKa = 9.78GGLGGRR160 pKa = 11.84NLRR163 pKa = 11.84GGLMM167 pKa = 3.7

Molecular weight: 17.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1583

68

600

226.1

25.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.443 ± 0.763

0.948 ± 0.437

4.422 ± 0.43

7.644 ± 1.233

2.906 ± 0.581

6.254 ± 1.656

2.211 ± 0.371

8.402 ± 1.573

9.097 ± 2.437

7.012 ± 0.574

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.095 ± 0.667

7.644 ± 0.359

3.474 ± 0.579

4.422 ± 0.425

5.496 ± 0.618

4.738 ± 0.792

7.265 ± 0.644

3.601 ± 1.308

1.958 ± 0.344

2.969 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski