Bacteroides nordii CL02T12C05
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4388 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I8XQ29|I8XQ29_9BACE Uncharacterized protein OS=Bacteroides nordii CL02T12C05 OX=997884 GN=HMPREF1068_01724 PE=4 SV=1
MM1 pKa = 7.52 IKK3 pKa = 10.02 IKK5 pKa = 10.61 FSILAVMLMTAFFSFSSCSDD25 pKa = 3.54 DD26 pKa = 4.55 DD27 pKa = 4.33 DD28 pKa = 4.61 TYY30 pKa = 11.66 LPPNQAIISALNQLYY45 pKa = 10.09 PGVKK49 pKa = 9.89 NIEE52 pKa = 3.96 WEE54 pKa = 4.3 QKK56 pKa = 8.89 GVYY59 pKa = 9.22 YY60 pKa = 10.84 VADD63 pKa = 3.47 CWVGNDD69 pKa = 4.34 EE70 pKa = 4.95 LDD72 pKa = 3.28 VWFDD76 pKa = 4.38 ANANWIMTEE85 pKa = 3.45 MDD87 pKa = 3.61 IFFEE91 pKa = 4.07 NLPTAVSTAYY101 pKa = 10.43 QEE103 pKa = 4.39 GNYY106 pKa = 9.83 ANWVIDD112 pKa = 4.66 DD113 pKa = 4.02 LTKK116 pKa = 10.36 LTFPQKK122 pKa = 9.66 PEE124 pKa = 3.73 EE125 pKa = 4.13 FVFEE129 pKa = 4.28 VQQGGQEE136 pKa = 3.7 RR137 pKa = 11.84 ALYY140 pKa = 8.34 YY141 pKa = 10.25 SEE143 pKa = 4.78 YY144 pKa = 11.09 GGLLRR149 pKa = 11.84 EE150 pKa = 4.46 KK151 pKa = 10.54 DD152 pKa = 3.63 ISDD155 pKa = 3.64 TDD157 pKa = 3.5 NTHH160 pKa = 6.28 WPDD163 pKa = 2.91 ISS165 pKa = 3.38
Molecular weight: 19.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.656
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|I9GJ01|I9GJ01_9BACE Uncharacterized protein OS=Bacteroides nordii CL02T12C05 OX=997884 GN=HMPREF1068_03477 PE=4 SV=1
MM1 pKa = 8.15 IIMVRR6 pKa = 11.84 YY7 pKa = 9.79 HH8 pKa = 6.92 NYY10 pKa = 7.11 QLKK13 pKa = 8.44 WRR15 pKa = 11.84 KK16 pKa = 9.56 EE17 pKa = 3.69 GMLLIRR23 pKa = 11.84 TFTFVIRR30 pKa = 11.84 CC31 pKa = 3.61
Molecular weight: 3.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.911
IPC_protein 10.687
Toseland 10.54
ProMoST 10.292
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.921
Grimsley 10.774
Solomon 10.789
Lehninger 10.76
Nozaki 10.54
DTASelect 10.423
Thurlkill 10.57
EMBOSS 10.935
Sillero 10.628
Patrickios 10.804
IPC_peptide 10.789
IPC2_peptide 9.589
IPC2.peptide.svr19 8.207
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4388
0
4388
1679095
23
2463
382.7
43.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.763 ± 0.042
1.215 ± 0.015
5.479 ± 0.021
6.523 ± 0.036
4.579 ± 0.021
6.84 ± 0.031
1.79 ± 0.015
7.117 ± 0.032
6.655 ± 0.032
9.013 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.651 ± 0.016
5.365 ± 0.035
3.724 ± 0.019
3.384 ± 0.016
4.463 ± 0.026
6.279 ± 0.034
5.82 ± 0.029
6.445 ± 0.03
1.279 ± 0.013
4.615 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here