Streptococcus phage Cp-1 (Bacteriophage Cp-1)
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q37986|Q37986_BPCP1 Orf2 protein OS=Streptococcus phage Cp-1 OX=10747 GN=orf2 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.12 MKK4 pKa = 10.54 ALYY7 pKa = 10.24 VNHH10 pKa = 6.73 NDD12 pKa = 3.07 EE13 pKa = 4.43 TFAEE17 pKa = 4.58 LTHH20 pKa = 6.2 GQALDD25 pKa = 3.79 VIFNADD31 pKa = 3.4 NAEE34 pKa = 4.09 MYY36 pKa = 10.46 GDD38 pKa = 4.05 HH39 pKa = 6.79 VNQIVDD45 pKa = 4.22 LGNEE49 pKa = 4.16 IKK51 pKa = 11.04 LEE53 pKa = 4.01 LYY55 pKa = 10.51 RR56 pKa = 11.84 NNEE59 pKa = 4.35 YY60 pKa = 10.52 IPFAGYY66 pKa = 7.91 YY67 pKa = 9.74 EE68 pKa = 4.25 RR69 pKa = 11.84 VKK71 pKa = 10.12 TLSEE75 pKa = 4.58 LYY77 pKa = 10.45 TNISDD82 pKa = 3.21 WFEE85 pKa = 3.78 EE86 pKa = 4.57 VEE88 pKa = 4.1
Molecular weight: 10.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.074
IPC2_protein 4.393
IPC_protein 4.266
Toseland 4.126
ProMoST 4.317
Dawson 4.215
Bjellqvist 4.431
Wikipedia 4.088
Rodwell 4.113
Grimsley 4.037
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.457
Thurlkill 4.139
EMBOSS 4.101
Sillero 4.38
Patrickios 1.977
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.295
Protein with the highest isoelectric point:
>tr|Q37995|Q37995_BPCP1 Connector protein OS=Streptococcus phage Cp-1 OX=10747 GN=orf10 PE=1 SV=1
MM1 pKa = 7.63 LRR3 pKa = 11.84 GLRR6 pKa = 11.84 IASMCTSLSLLMIDD20 pKa = 3.97 LAEE23 pKa = 4.07 IRR25 pKa = 11.84 KK26 pKa = 8.57 ATGLRR31 pKa = 11.84 PLFAPSPVNFSKK43 pKa = 11.02 SSTTIVRR50 pKa = 11.84 ASTLALSMLKK60 pKa = 10.47 ALASNMSRR68 pKa = 11.84 CVEE71 pKa = 3.86 NSAEE75 pKa = 3.99 ITRR78 pKa = 11.84 YY79 pKa = 7.56 WSSIAVMFGTPTGLLNVVRR98 pKa = 11.84 LGIVKK103 pKa = 10.39 RR104 pKa = 11.84 LDD106 pKa = 3.12 KK107 pKa = 11.05 LIRR110 pKa = 11.84 DD111 pKa = 3.91 WLKK114 pKa = 10.08 STTKK118 pKa = 10.11 SCFVSSIFVPATVIFLSLPFKK139 pKa = 10.78 VCIRR143 pKa = 11.84 DD144 pKa = 3.46 FRR146 pKa = 11.84 KK147 pKa = 10.24
Molecular weight: 16.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.663
IPC_protein 10.292
Toseland 10.994
ProMoST 10.745
Dawson 11.038
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.301
Grimsley 11.052
Solomon 11.213
Lehninger 11.169
Nozaki 10.979
DTASelect 10.73
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.979
Patrickios 11.038
IPC_peptide 11.213
IPC2_peptide 9.926
IPC2.peptide.svr19 8.777
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
5916
62
586
211.3
24.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.254 ± 0.371
0.828 ± 0.122
5.882 ± 0.368
6.964 ± 0.404
5.646 ± 0.406
5.24 ± 0.503
1.437 ± 0.171
7.066 ± 0.381
7.809 ± 0.509
8.384 ± 0.503
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.201
6.863 ± 0.58
2.738 ± 0.329
3.803 ± 0.29
3.989 ± 0.411
6.423 ± 0.52
6.085 ± 0.491
5.882 ± 0.31
1.369 ± 0.257
5.054 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here