Changjiang tombus-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG20|A0A1L3KG20_9VIRU Capsid protein OS=Changjiang tombus-like virus 8 OX=1922822 PE=3 SV=1
MM1 pKa = 6.36VTTRR5 pKa = 11.84ANSEE9 pKa = 4.09FLPVTRR15 pKa = 11.84LSQPDD20 pKa = 3.93DD21 pKa = 3.45QPYY24 pKa = 6.93WTFWDD29 pKa = 3.36RR30 pKa = 11.84WKK32 pKa = 11.26SSFAEE37 pKa = 4.54FWSTSTITRR46 pKa = 11.84MCCCVDD52 pKa = 3.69PEE54 pKa = 4.36VHH56 pKa = 7.16LDD58 pKa = 3.14AQLRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84QHH66 pKa = 4.96NAVRR70 pKa = 11.84EE71 pKa = 4.2SLLYY75 pKa = 9.68TLDD78 pKa = 3.44YY79 pKa = 11.17RR80 pKa = 11.84GDD82 pKa = 3.54TSIITATMNDD92 pKa = 4.24LLDD95 pKa = 4.31EE96 pKa = 4.38GHH98 pKa = 7.21VMLQDD103 pKa = 4.17AAPTPARR110 pKa = 11.84SPPTINPATLGPTFTTLTVYY130 pKa = 10.54EE131 pKa = 4.7PATHH135 pKa = 6.53HH136 pKa = 6.64AWSQPQLAPPLNHH149 pKa = 6.2TRR151 pKa = 11.84RR152 pKa = 11.84NLTPVKK158 pKa = 10.0FVPRR162 pKa = 11.84FTAAVIVEE170 pKa = 3.98LRR172 pKa = 11.84SRR174 pKa = 11.84LGQLPEE180 pKa = 4.67SIPGNALIVEE190 pKa = 4.61RR191 pKa = 11.84EE192 pKa = 4.09ALRR195 pKa = 11.84LMRR198 pKa = 11.84KK199 pKa = 8.84YY200 pKa = 10.67SVRR203 pKa = 11.84EE204 pKa = 3.69VDD206 pKa = 4.97AVTHH210 pKa = 6.29LPSIISCYY218 pKa = 9.45FRR220 pKa = 11.84EE221 pKa = 4.23DD222 pKa = 2.74LHH224 pKa = 7.9YY225 pKa = 10.49RR226 pKa = 11.84VEE228 pKa = 4.45TSVSRR233 pKa = 11.84MSKK236 pKa = 8.08FQRR239 pKa = 11.84WLVGEE244 pKa = 4.3AQPKK248 pKa = 10.11PSFTPLAA255 pKa = 4.35

Molecular weight: 29.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG20|A0A1L3KG20_9VIRU Capsid protein OS=Changjiang tombus-like virus 8 OX=1922822 PE=3 SV=1
MM1 pKa = 7.55TNKK4 pKa = 9.29QQPKK8 pKa = 9.74KK9 pKa = 10.4GNLRR13 pKa = 11.84GAQQKK18 pKa = 10.74NSVPTMARR26 pKa = 11.84NDD28 pKa = 3.7SVRR31 pKa = 11.84AAPVALATRR40 pKa = 11.84RR41 pKa = 11.84NGTAARR47 pKa = 11.84MTTTNTGVTVTHH59 pKa = 7.22RR60 pKa = 11.84SFLMPVTNTLNYY72 pKa = 8.7TAFSVPCNPGMAGSFPWLCKK92 pKa = 9.31LARR95 pKa = 11.84RR96 pKa = 11.84YY97 pKa = 9.71EE98 pKa = 3.97EE99 pKa = 3.91YY100 pKa = 10.38RR101 pKa = 11.84FKK103 pKa = 11.01KK104 pKa = 9.95LRR106 pKa = 11.84YY107 pKa = 7.71EE108 pKa = 4.19FRR110 pKa = 11.84SVTASSQSGVVMMSFDD126 pKa = 3.91FDD128 pKa = 4.15AADD131 pKa = 3.72SAPSTKK137 pKa = 10.33AEE139 pKa = 4.05QAQTVPNTEE148 pKa = 3.96TNVWMNNDD156 pKa = 3.58LVVTPDD162 pKa = 3.93PKK164 pKa = 9.04WQYY167 pKa = 9.92VRR169 pKa = 11.84SGFLSSNLDD178 pKa = 2.87IKK180 pKa = 10.14TYY182 pKa = 11.54DD183 pKa = 3.84MGNLWLSTAYY193 pKa = 10.41GNNVVGGEE201 pKa = 4.33LYY203 pKa = 10.69VEE205 pKa = 4.24YY206 pKa = 9.78TVEE209 pKa = 4.14LRR211 pKa = 11.84KK212 pKa = 9.0PTDD215 pKa = 3.63GPEE218 pKa = 4.09TCGTWTADD226 pKa = 2.94TGAFSAPVNQTNVVSTGASFPFRR249 pKa = 11.84RR250 pKa = 11.84IDD252 pKa = 3.39NNNFEE257 pKa = 4.52VVSGGEE263 pKa = 3.96YY264 pKa = 10.46IMVCRR269 pKa = 11.84ASGTGLTAVIATPTIASSGTTALVSSIQTCVGGSSTINVLRR310 pKa = 11.84LRR312 pKa = 11.84VDD314 pKa = 3.23TGDD317 pKa = 3.37VIYY320 pKa = 9.9FANAGTGTTINATRR334 pKa = 11.84YY335 pKa = 7.16WVAPVDD341 pKa = 3.71YY342 pKa = 9.99TSIVV346 pKa = 3.09

Molecular weight: 37.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1124

255

523

374.7

42.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.74 ± 0.477

2.135 ± 0.427

4.359 ± 0.119

4.715 ± 0.56

3.915 ± 0.066

5.872 ± 0.966

2.224 ± 0.71

3.381 ± 0.188

3.915 ± 0.856

7.74 ± 0.911

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.203

5.071 ± 0.811

5.516 ± 0.905

3.559 ± 0.242

7.117 ± 0.569

6.673 ± 0.779

8.185 ± 2.157

9.164 ± 0.585

1.423 ± 0.394

4.359 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski