Rosa rugosa leaf distortion virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Pelarspovirus

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1P1T0|M1P1T0_9TOMB Replicase protein OS=Rosa rugosa leaf distortion virus OX=535898 PE=4 SV=1
MM1 pKa = 7.5SLFFEE6 pKa = 4.88LAYY9 pKa = 9.54LTAKK13 pKa = 10.56AGFVVAKK20 pKa = 9.88EE21 pKa = 3.98LALLATQIRR30 pKa = 11.84NDD32 pKa = 4.2EE33 pKa = 4.08IVHH36 pKa = 6.6IDD38 pKa = 3.41VQTDD42 pKa = 3.07RR43 pKa = 11.84VRR45 pKa = 11.84RR46 pKa = 11.84VFGIQGRR53 pKa = 11.84EE54 pKa = 3.93IIPTSDD60 pKa = 3.38CTDD63 pKa = 4.01LLNATQDD70 pKa = 3.87LVVADD75 pKa = 4.39PDD77 pKa = 3.69LHH79 pKa = 8.17EE80 pKa = 4.74EE81 pKa = 4.18EE82 pKa = 4.7CLEE85 pKa = 5.09DD86 pKa = 3.15IKK88 pKa = 11.59EE89 pKa = 4.09EE90 pKa = 4.19VKK92 pKa = 11.1GDD94 pKa = 3.56GGEE97 pKa = 4.12VSQKK101 pKa = 7.88VVRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84VRR108 pKa = 11.84KK109 pKa = 9.35KK110 pKa = 10.97LPFACILAADD120 pKa = 4.66AKK122 pKa = 9.98NHH124 pKa = 6.43FGGVPSACRR133 pKa = 11.84ANEE136 pKa = 3.91LSVTKK141 pKa = 10.59YY142 pKa = 10.44LVSKK146 pKa = 10.57CKK148 pKa = 9.47EE149 pKa = 3.71RR150 pKa = 11.84KK151 pKa = 8.56LTVLQTRR158 pKa = 11.84QVSSLAFCLVFTPDD172 pKa = 3.74SNDD175 pKa = 2.75KK176 pKa = 10.89SIYY179 pKa = 9.94QFLNSEE185 pKa = 4.51SVFEE189 pKa = 4.4RR190 pKa = 11.84RR191 pKa = 11.84CDD193 pKa = 3.67YY194 pKa = 11.09LKK196 pKa = 10.52SQSVEE201 pKa = 4.29SCWMQLFSSPWNPKK215 pKa = 7.28AWRR218 pKa = 11.84RR219 pKa = 11.84VILRR223 pKa = 11.84LSSLGPQEE231 pKa = 4.09AFRR234 pKa = 11.84FVKK237 pKa = 10.62

Molecular weight: 26.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1NMF8|M1NMF8_9TOMB RNA-directed RNA polymerase OS=Rosa rugosa leaf distortion virus OX=535898 PE=4 SV=1
MM1 pKa = 7.01ATIMLSGWISTGVNTLLSLKK21 pKa = 10.63SRR23 pKa = 11.84DD24 pKa = 3.29IVFTSRR30 pKa = 11.84LGSARR35 pKa = 11.84ISKK38 pKa = 9.24LLWKK42 pKa = 8.71MHH44 pKa = 5.07IRR46 pKa = 11.84SWSLYY51 pKa = 8.92SNSPHH56 pKa = 7.33LEE58 pKa = 4.15TKK60 pKa = 9.32STFLNGQSTTQSS72 pKa = 3.05

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1584

60

761

264.0

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.716

2.525 ± 0.615

4.609 ± 0.439

5.808 ± 1.156

4.987 ± 0.377

5.934 ± 0.438

2.146 ± 0.452

3.851 ± 0.487

5.997 ± 0.297

8.902 ± 0.772

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.578 ± 0.315

3.977 ± 0.631

4.419 ± 0.559

4.04 ± 0.288

6.881 ± 0.822

9.091 ± 1.942

5.24 ± 1.389

8.775 ± 0.914

1.263 ± 0.367

2.904 ± 0.423
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski