Rhodoblastus sphagnicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Rhodoblastus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6NAX3|A0A2S6NAX3_9RHIZ Parvulin-like PPIase OS=Rhodoblastus sphagnicola OX=333368 GN=CCR94_08145 PE=3 SV=1
MM1 pKa = 7.63AGCAIGGDD9 pKa = 3.76DD10 pKa = 5.12SLNLSITRR18 pKa = 11.84PWLWADD24 pKa = 3.82DD25 pKa = 3.78KK26 pKa = 10.9VTSKK30 pKa = 10.32MYY32 pKa = 11.07GDD34 pKa = 4.25AGGNMSDD41 pKa = 4.1HH42 pKa = 6.64SVGGNDD48 pKa = 3.3VLSVYY53 pKa = 10.93LEE55 pKa = 4.15DD56 pKa = 3.89FQYY59 pKa = 11.3LRR61 pKa = 11.84VDD63 pKa = 3.41MFGDD67 pKa = 3.51AGGSLAGYY75 pKa = 10.5ASGGNDD81 pKa = 2.99TFSSDD86 pKa = 3.56THH88 pKa = 6.04WGHH91 pKa = 6.19IAVLIMYY98 pKa = 9.77GDD100 pKa = 3.95AGLNISGHH108 pKa = 5.94AFGGNDD114 pKa = 3.54VFNLGANVEE123 pKa = 4.41GNITCYY129 pKa = 10.87GDD131 pKa = 3.53AGGTMSGYY139 pKa = 9.15ATGGDD144 pKa = 3.85DD145 pKa = 4.27EE146 pKa = 4.75FTTAHH151 pKa = 5.05VRR153 pKa = 11.84EE154 pKa = 4.62TNFSIYY160 pKa = 10.64GDD162 pKa = 3.64AGGDD166 pKa = 3.32MSNYY170 pKa = 8.51ATGGNDD176 pKa = 2.85VFSANGEE183 pKa = 3.88NGYY186 pKa = 9.88VLFYY190 pKa = 11.3GDD192 pKa = 4.41AGGNMTGHH200 pKa = 6.53AQGGDD205 pKa = 3.33DD206 pKa = 4.48TYY208 pKa = 11.86NAGNDD213 pKa = 3.38ATTTFYY219 pKa = 11.65GDD221 pKa = 3.1AGGNMGDD228 pKa = 3.55RR229 pKa = 11.84TVGGNDD235 pKa = 3.31TFIGNAANFCGGVLAYY251 pKa = 10.55GDD253 pKa = 4.61ALTMAGSARR262 pKa = 11.84GGNDD266 pKa = 3.16TLVGASFSPTGNTLYY281 pKa = 11.21GDD283 pKa = 4.51ALTMSGNARR292 pKa = 11.84GGNDD296 pKa = 3.39TLIGGDD302 pKa = 3.63GVAAISGKK310 pKa = 9.88YY311 pKa = 9.37EE312 pKa = 4.38SILIGDD318 pKa = 4.84AKK320 pKa = 9.93TLSGNARR327 pKa = 11.84GGDD330 pKa = 3.54DD331 pKa = 3.62TLISGGGSDD340 pKa = 5.86DD341 pKa = 3.05MWGDD345 pKa = 3.18ARR347 pKa = 11.84VIGDD351 pKa = 3.75SARR354 pKa = 11.84GGDD357 pKa = 3.47DD358 pKa = 3.16RR359 pKa = 11.84FVFKK363 pKa = 10.86AGNGHH368 pKa = 7.08DD369 pKa = 4.37AIEE372 pKa = 5.24DD373 pKa = 4.01FGQGQAGSKK382 pKa = 9.24WGTDD386 pKa = 3.7HH387 pKa = 7.49IDD389 pKa = 3.17VSALGIQSFGALNISAFDD407 pKa = 3.89TATHH411 pKa = 6.71TSTLTFSAGNDD422 pKa = 3.47VVVHH426 pKa = 6.12SMLALRR432 pKa = 11.84AQDD435 pKa = 4.41FIFGG439 pKa = 3.78

Molecular weight: 44.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6N304|A0A2S6N304_9RHIZ Uncharacterized protein OS=Rhodoblastus sphagnicola OX=333368 GN=CCR94_16425 PE=4 SV=1
MM1 pKa = 7.93IPIPPKK7 pKa = 9.81NRR9 pKa = 11.84PAVRR13 pKa = 11.84SHH15 pKa = 4.52FTGRR19 pKa = 11.84GGFRR23 pKa = 11.84RR24 pKa = 11.84VLVQKK29 pKa = 10.56ARR31 pKa = 11.84RR32 pKa = 11.84SPRR35 pKa = 11.84LRR37 pKa = 11.84SNIARR42 pKa = 11.84RR43 pKa = 11.84FSAQAPVQHH52 pKa = 6.56GAAIICRR59 pKa = 11.84AKK61 pKa = 10.5SRR63 pKa = 11.84PQGAA67 pKa = 3.36

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4616

0

4616

1435672

25

4372

311.0

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.729 ± 0.058

0.961 ± 0.014

5.67 ± 0.027

5.613 ± 0.044

3.9 ± 0.027

8.383 ± 0.053

1.941 ± 0.018

4.885 ± 0.024

3.516 ± 0.03

10.311 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.017

2.653 ± 0.025

5.188 ± 0.034

3.087 ± 0.021

7.264 ± 0.046

5.497 ± 0.038

4.868 ± 0.036

7.076 ± 0.027

1.24 ± 0.015

2.093 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski