Microviridae sp.

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345N1A1|A0A345N1A1_9VIRU Major capsid protein OS=Microviridae sp. OX=2202644 PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.52LFDD5 pKa = 3.7QVAVKK10 pKa = 10.32SPKK13 pKa = 9.47YY14 pKa = 10.4SKK16 pKa = 10.72FDD18 pKa = 3.68LSHH21 pKa = 5.89EE22 pKa = 4.5RR23 pKa = 11.84KK24 pKa = 9.79FSMKK28 pKa = 10.17HH29 pKa = 4.1GTLTPIYY36 pKa = 9.11LQEE39 pKa = 4.11VLPGDD44 pKa = 3.97SFRR47 pKa = 11.84LNSEE51 pKa = 3.7MLIRR55 pKa = 11.84LAPMKK60 pKa = 10.55FPVLHH65 pKa = 6.89RR66 pKa = 11.84ISAYY70 pKa = 7.31IHH72 pKa = 5.9YY73 pKa = 9.16WFVPNRR79 pKa = 11.84LVWSEE84 pKa = 3.93WEE86 pKa = 4.12DD87 pKa = 4.53FITGGDD93 pKa = 4.05DD94 pKa = 3.61GLSAPLFPVIDD105 pKa = 4.01EE106 pKa = 4.67TNLTTSQAKK115 pKa = 10.28AGEE118 pKa = 4.27LWDD121 pKa = 4.31FMGMPPIAGTVNPKK135 pKa = 9.57MKK137 pKa = 10.41INALPFRR144 pKa = 11.84GYY146 pKa = 9.21ATIYY150 pKa = 10.34NEE152 pKa = 4.73FYY154 pKa = 10.6RR155 pKa = 11.84DD156 pKa = 3.19QNLQDD161 pKa = 4.8KK162 pKa = 10.91IPVTKK167 pKa = 10.08TGGSLGSAEE176 pKa = 4.07VTEE179 pKa = 4.23LLKK182 pKa = 10.84LRR184 pKa = 11.84YY185 pKa = 9.14RR186 pKa = 11.84AWEE189 pKa = 3.66HH190 pKa = 7.73DD191 pKa = 4.22YY192 pKa = 9.43FTSCLPQAQKK202 pKa = 10.86GGEE205 pKa = 4.04VLLPMEE211 pKa = 5.03ADD213 pKa = 2.92INYY216 pKa = 9.8KK217 pKa = 10.11SEE219 pKa = 3.54ASIRR223 pKa = 11.84MATSSAGYY231 pKa = 8.35TEE233 pKa = 4.4GANVQIQQTGFTPPTAGLFGMEE255 pKa = 4.26GSTARR260 pKa = 11.84AFGIEE265 pKa = 4.21NIEE268 pKa = 4.56SIDD271 pKa = 4.03NATTTINDD279 pKa = 2.88LRR281 pKa = 11.84KK282 pKa = 9.59AVRR285 pKa = 11.84LQEE288 pKa = 4.12FLEE291 pKa = 4.19KK292 pKa = 10.71AMRR295 pKa = 11.84AGSRR299 pKa = 11.84YY300 pKa = 9.49SEE302 pKa = 4.35SIKK305 pKa = 10.64AYY307 pKa = 9.99FGVRR311 pKa = 11.84SSDD314 pKa = 3.12ARR316 pKa = 11.84LQRR319 pKa = 11.84PEE321 pKa = 3.79FLGGGVQPIVVSEE334 pKa = 3.99VLQTYY339 pKa = 9.8EE340 pKa = 4.06GTDD343 pKa = 3.54PLGKK347 pKa = 9.56MGGHH351 pKa = 7.38GISVGNKK358 pKa = 8.83SGFKK362 pKa = 10.26KK363 pKa = 10.25RR364 pKa = 11.84FEE366 pKa = 3.83EE367 pKa = 3.73HH368 pKa = 6.69GYY370 pKa = 10.3IIGIMSIIPKK380 pKa = 9.51SAYY383 pKa = 9.56GQGIPKK389 pKa = 9.19TFMKK393 pKa = 10.55ANRR396 pKa = 11.84YY397 pKa = 9.55DD398 pKa = 3.43FAFPEE403 pKa = 4.77FAQLGEE409 pKa = 4.09QEE411 pKa = 4.43VLNKK415 pKa = 10.06EE416 pKa = 4.5VFWDD420 pKa = 3.68EE421 pKa = 3.92TDD423 pKa = 4.29VNNVWNDD430 pKa = 3.28LEE432 pKa = 4.23FGYY435 pKa = 10.27IPRR438 pKa = 11.84YY439 pKa = 9.74SEE441 pKa = 4.12YY442 pKa = 10.8KK443 pKa = 9.56FNNSSVHH450 pKa = 4.71GQFRR454 pKa = 11.84PGEE457 pKa = 4.13TLEE460 pKa = 3.76NWTMVRR466 pKa = 11.84KK467 pKa = 10.02FEE469 pKa = 4.53NRR471 pKa = 11.84PALNEE476 pKa = 3.93EE477 pKa = 4.79FISSEE482 pKa = 4.77DD483 pKa = 3.25ATQDD487 pKa = 2.92IFNVTDD493 pKa = 4.06PNEE496 pKa = 4.13DD497 pKa = 3.45KK498 pKa = 11.38YY499 pKa = 11.18FVQIYY504 pKa = 10.02HH505 pKa = 6.75NISAIRR511 pKa = 11.84ALPYY515 pKa = 10.48FGTPTLL521 pKa = 3.89

Molecular weight: 58.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345N1A1|A0A345N1A1_9VIRU Major capsid protein OS=Microviridae sp. OX=2202644 PE=3 SV=1
MM1 pKa = 7.29ACEE4 pKa = 4.01RR5 pKa = 11.84PIILKK10 pKa = 10.33DD11 pKa = 3.3HH12 pKa = 6.69LTTVPCRR19 pKa = 11.84RR20 pKa = 11.84CIFCLQEE27 pKa = 5.46KK28 pKa = 9.61RR29 pKa = 11.84DD30 pKa = 3.4NWTFRR35 pKa = 11.84VGQEE39 pKa = 3.77YY40 pKa = 10.64KK41 pKa = 10.42KK42 pKa = 10.98ARR44 pKa = 11.84TAYY47 pKa = 10.26FITLTYY53 pKa = 10.67DD54 pKa = 3.66DD55 pKa = 3.76QHH57 pKa = 8.61LPIDD61 pKa = 4.15NKK63 pKa = 10.35GRR65 pKa = 11.84AYY67 pKa = 10.78LDD69 pKa = 3.23KK70 pKa = 10.55KK71 pKa = 10.53QLVGFLKK78 pKa = 10.49RR79 pKa = 11.84LRR81 pKa = 11.84IRR83 pKa = 11.84NEE85 pKa = 3.79RR86 pKa = 11.84EE87 pKa = 3.3IEE89 pKa = 4.17KK90 pKa = 10.27YY91 pKa = 8.47WRR93 pKa = 11.84ARR95 pKa = 11.84EE96 pKa = 3.91NSTPMPKK103 pKa = 9.0MAKK106 pKa = 8.59TKK108 pKa = 10.46YY109 pKa = 8.97FACGEE114 pKa = 4.14YY115 pKa = 10.53GSKK118 pKa = 10.2KK119 pKa = 9.72KK120 pKa = 10.24RR121 pKa = 11.84PHH123 pKa = 4.34YY124 pKa = 10.22HH125 pKa = 6.31IIVFNLFPNTLKK137 pKa = 10.83YY138 pKa = 10.88LNEE141 pKa = 3.97LWTEE145 pKa = 4.05GNIDD149 pKa = 4.22LKK151 pKa = 10.58IAKK154 pKa = 9.59AGAIHH159 pKa = 5.6YY160 pKa = 7.72TSGYY164 pKa = 9.87LLKK167 pKa = 9.37QTAVIKK173 pKa = 10.46DD174 pKa = 2.82GHH176 pKa = 6.06ARR178 pKa = 11.84QFIIASQHH186 pKa = 5.13MGEE189 pKa = 4.89HH190 pKa = 5.44YY191 pKa = 10.51LKK193 pKa = 10.46NAMYY197 pKa = 10.34HH198 pKa = 5.69SEE200 pKa = 4.21NLTAKK205 pKa = 10.41CSINGFSIGLPSNFRR220 pKa = 11.84NKK222 pKa = 9.82IFTKK226 pKa = 7.62EE227 pKa = 3.65QKK229 pKa = 10.49EE230 pKa = 3.92IMRR233 pKa = 11.84NEE235 pKa = 3.95NEE237 pKa = 4.32KK238 pKa = 10.75KK239 pKa = 10.29RR240 pKa = 11.84LEE242 pKa = 4.57KK243 pKa = 10.12DD244 pKa = 3.73TIWIDD249 pKa = 3.29QKK251 pKa = 9.88TKK253 pKa = 11.04EE254 pKa = 4.57NIDD257 pKa = 3.27FDD259 pKa = 4.72KK260 pKa = 11.5YY261 pKa = 8.05QTEE264 pKa = 4.2QKK266 pKa = 11.0NLLRR270 pKa = 11.84EE271 pKa = 4.13NANKK275 pKa = 8.63KK276 pKa = 7.24TSKK279 pKa = 9.94KK280 pKa = 10.2RR281 pKa = 11.84KK282 pKa = 8.82LL283 pKa = 3.42

Molecular weight: 33.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1422

107

521

284.4

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.048 ± 0.924

1.055 ± 0.608

4.712 ± 0.472

7.314 ± 0.659

4.782 ± 0.783

6.399 ± 1.063

2.25 ± 0.576

7.736 ± 0.768

8.509 ± 1.497

9.705 ± 2.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.883 ± 0.408

6.188 ± 0.811

3.797 ± 0.692

4.993 ± 0.692

5.345 ± 0.552

4.712 ± 0.82

4.993 ± 0.919

3.797 ± 0.718

1.336 ± 0.202

3.446 ± 0.667
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski