Chitinophaga barathri

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N4MFU6|A0A3N4MFU6_9BACT SDR family NAD(P)-dependent oxidoreductase OS=Chitinophaga barathri OX=1647451 GN=EG028_00980 PE=3 SV=1
MM1 pKa = 7.42LKK3 pKa = 10.27DD4 pKa = 4.32PPPLPPSAWNSLFTQLSAQGSTFNTPLSASDD35 pKa = 3.29WYY37 pKa = 10.87FAFLSSVDD45 pKa = 3.53QDD47 pKa = 3.87EE48 pKa = 4.76KK49 pKa = 11.29NGLYY53 pKa = 10.27YY54 pKa = 11.43VMFEE58 pKa = 4.22VVPKK62 pKa = 9.82EE63 pKa = 3.85WVDD66 pKa = 3.58NVIMSGDD73 pKa = 3.56ADD75 pKa = 3.62ISTLAPVSPASCYY88 pKa = 9.05WINGVTEE95 pKa = 4.04NVILNALGLKK105 pKa = 10.37SPDD108 pKa = 3.38EE109 pKa = 4.13LAVGSWYY116 pKa = 10.95VMQLKK121 pKa = 10.32KK122 pKa = 10.1GTSGISGDD130 pKa = 5.02LYY132 pKa = 11.4DD133 pKa = 5.65PDD135 pKa = 5.74LDD137 pKa = 4.29MEE139 pKa = 4.93FDD141 pKa = 3.53TT142 pKa = 6.14

Molecular weight: 15.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N4M7U6|A0A3N4M7U6_9BACT RNA polymerase sigma-70 factor OS=Chitinophaga barathri OX=1647451 GN=EG028_18435 PE=3 SV=1
MM1 pKa = 7.27QKK3 pKa = 10.13IIPNRR8 pKa = 11.84MVIFARR14 pKa = 11.84DD15 pKa = 3.54VQNITGRR22 pKa = 11.84SEE24 pKa = 3.75RR25 pKa = 11.84ASRR28 pKa = 11.84LLLQRR33 pKa = 11.84IRR35 pKa = 11.84QALDD39 pKa = 2.86KK40 pKa = 10.89KK41 pKa = 10.17AGQFISIAEE50 pKa = 4.22FCRR53 pKa = 11.84YY54 pKa = 8.15TGLSEE59 pKa = 4.32EE60 pKa = 4.42EE61 pKa = 3.82VRR63 pKa = 11.84SYY65 pKa = 11.71LNRR68 pKa = 4.16

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5481

0

5481

2048369

13

7902

373.7

41.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.204 ± 0.037

0.801 ± 0.013

5.199 ± 0.022

5.591 ± 0.038

4.674 ± 0.02

7.46 ± 0.035

1.922 ± 0.019

6.275 ± 0.027

5.948 ± 0.043

9.359 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.016

5.035 ± 0.036

4.393 ± 0.019

3.866 ± 0.025

4.845 ± 0.028

5.975 ± 0.028

6.04 ± 0.063

6.545 ± 0.03

1.347 ± 0.012

4.06 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski