Macaca nemestrina rhadinovirus 2
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5CYF8|A0A0B5CYF8_9GAMA ORF49 OS=Macaca nemestrina rhadinovirus 2 OX=123630 GN=ORF49 PE=4 SV=1
MM1 pKa = 7.9 AMFLSDD7 pKa = 4.33 PPRR10 pKa = 11.84 TPPATPRR17 pKa = 11.84 MLPIPGAPKK26 pKa = 9.82 KK27 pKa = 9.09 RR28 pKa = 11.84 TRR30 pKa = 11.84 RR31 pKa = 11.84 FLFAGAGTALPVPPGYY47 pKa = 10.1 QGPRR51 pKa = 11.84 VIDD54 pKa = 3.3 MSAPDD59 pKa = 3.91 SVFDD63 pKa = 4.91 LDD65 pKa = 5.44 SPPTTPKK72 pKa = 10.49 TPDD75 pKa = 3.2 EE76 pKa = 4.19 TDD78 pKa = 3.13 SRR80 pKa = 11.84 SEE82 pKa = 3.87 DD83 pKa = 3.52 SNYY86 pKa = 10.75 DD87 pKa = 3.43 DD88 pKa = 5.41 SDD90 pKa = 3.75 VDD92 pKa = 3.74 EE93 pKa = 4.79 EE94 pKa = 5.09 TNTEE98 pKa = 4.24 PPVSSPPKK106 pKa = 9.67 IGLALSEE113 pKa = 4.37 GDD115 pKa = 4.21 LFRR118 pKa = 11.84 PSNTRR123 pKa = 11.84 PSVIVEE129 pKa = 4.35 SGPVAQPNAPAPLTAFGGPRR149 pKa = 11.84 PVAVVTGQHH158 pKa = 6.35 RR159 pKa = 11.84 APPSSDD165 pKa = 3.45 SNSDD169 pKa = 3.36 DD170 pKa = 4.48 FFIDD174 pKa = 4.09 DD175 pKa = 4.5 YY176 pKa = 11.62 EE177 pKa = 6.35 DD178 pKa = 3.92 NDD180 pKa = 3.91 EE181 pKa = 4.95 SDD183 pKa = 4.91 NDD185 pKa = 4.14 DD186 pKa = 3.71 EE187 pKa = 4.92 TDD189 pKa = 3.36 GFSPRR194 pKa = 11.84 GSTGPWPGDD203 pKa = 3.44 VSRR206 pKa = 11.84 SPPEE210 pKa = 4.42 GDD212 pKa = 2.92 WSSDD216 pKa = 3.41 DD217 pKa = 4.93 DD218 pKa = 3.96 LTEE221 pKa = 3.98 EE222 pKa = 4.03 TGAAAGEE229 pKa = 4.03 EE230 pKa = 4.31 TIIISSSDD238 pKa = 3.96 DD239 pKa = 3.61 EE240 pKa = 6.71 DD241 pKa = 5.37 EE242 pKa = 5.68 DD243 pKa = 4.17 DD244 pKa = 3.92 QNSVGTEE251 pKa = 3.91 DD252 pKa = 4.96 EE253 pKa = 4.13 FDD255 pKa = 3.89 ANAGSGGADD264 pKa = 3.46 VIDD267 pKa = 4.57 LCSSSDD273 pKa = 3.5 SDD275 pKa = 5.13 DD276 pKa = 3.96 EE277 pKa = 4.41 VDD279 pKa = 3.75 GAVGGARR286 pKa = 11.84 ANCKK290 pKa = 9.71 RR291 pKa = 11.84 RR292 pKa = 11.84 ASRR295 pKa = 11.84 LDD297 pKa = 3.98 DD298 pKa = 4.85 DD299 pKa = 5.05 SEE301 pKa = 5.72 DD302 pKa = 4.32 DD303 pKa = 3.75 IIYY306 pKa = 10.83 VGTTQGRR313 pKa = 11.84 KK314 pKa = 9.15 RR315 pKa = 11.84 RR316 pKa = 11.84 VTSTAKK322 pKa = 10.63 GGATSSSEE330 pKa = 3.94 GAGVSGRR337 pKa = 11.84 QNMAATPPVCGNDD350 pKa = 3.26 NYY352 pKa = 10.38 PWPWLDD358 pKa = 3.15
Molecular weight: 37.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.285
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.986
Patrickios 3.821
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A0B5D3J3|A0A0B5D3J3_9GAMA Major capsid protein OS=Macaca nemestrina rhadinovirus 2 OX=123630 GN=ORF25 PE=3 SV=1
MM1 pKa = 7.48 SLDD4 pKa = 3.4 NQHH7 pKa = 6.07 RR8 pKa = 11.84 HH9 pKa = 4.71 SAALEE14 pKa = 4.0 CLPTARR20 pKa = 11.84 KK21 pKa = 9.03 RR22 pKa = 11.84 AGIRR26 pKa = 11.84 AHH28 pKa = 5.85 LAVYY32 pKa = 10.09 RR33 pKa = 11.84 RR34 pKa = 11.84 LIKK37 pKa = 10.3 HH38 pKa = 6.52 RR39 pKa = 11.84 SLGDD43 pKa = 2.99 IFKK46 pKa = 10.49 FLSICSTRR54 pKa = 11.84 EE55 pKa = 3.79 ATEE58 pKa = 4.05 DD59 pKa = 3.15 AQFRR63 pKa = 11.84 IFFEE67 pKa = 4.12 VTLGKK72 pKa = 10.47 RR73 pKa = 11.84 IADD76 pKa = 3.44 CVLTVEE82 pKa = 4.97 SKK84 pKa = 10.16 NQKK87 pKa = 6.26 TCYY90 pKa = 9.65 VVEE93 pKa = 4.86 LKK95 pKa = 9.6 TCLSGFVFPGSAIKK109 pKa = 10.43 ISQRR113 pKa = 11.84 RR114 pKa = 11.84 QGLEE118 pKa = 3.57 QLTDD122 pKa = 3.31 SVAYY126 pKa = 9.51 IGRR129 pKa = 11.84 AAPKK133 pKa = 10.09 GHH135 pKa = 6.51 EE136 pKa = 4.15 NWSVRR141 pKa = 11.84 PLLLFKK147 pKa = 10.55 NQKK150 pKa = 5.53 TLKK153 pKa = 8.91 TIHH156 pKa = 6.4 TEE158 pKa = 3.89 SSAFPPTFINTTSVALNGFFNQWEE182 pKa = 4.24 DD183 pKa = 3.25 ARR185 pKa = 11.84 VRR187 pKa = 11.84 KK188 pKa = 8.62 MLYY191 pKa = 9.97 AIPAKK196 pKa = 9.58 THH198 pKa = 5.32 ATNNLEE204 pKa = 4.21 FLDD207 pKa = 4.34 PASKK211 pKa = 10.09 QRR213 pKa = 11.84 SVYY216 pKa = 10.4 SQTIVNGGKK225 pKa = 9.91 KK226 pKa = 9.79 KK227 pKa = 10.08 RR228 pKa = 11.84 VRR230 pKa = 11.84 DD231 pKa = 3.75 AKK233 pKa = 10.05 STAGAQGSRR242 pKa = 11.84 AEE244 pKa = 4.74 KK245 pKa = 10.38 KK246 pKa = 9.19 PAPARR251 pKa = 11.84 ARR253 pKa = 11.84 QRR255 pKa = 11.84 AANAPTGNGDD265 pKa = 3.35 GYY267 pKa = 11.32 ARR269 pKa = 11.84 HH270 pKa = 6.62 RR271 pKa = 11.84 DD272 pKa = 3.69 DD273 pKa = 4.01 PRR275 pKa = 11.84 HH276 pKa = 5.03 GRR278 pKa = 11.84 RR279 pKa = 11.84 FASTEE284 pKa = 3.51 QKK286 pKa = 9.74 PRR288 pKa = 11.84 RR289 pKa = 11.84 NRR291 pKa = 11.84 TVNRR295 pKa = 11.84 PATQNRR301 pKa = 11.84 PSDD304 pKa = 2.92 AWRR307 pKa = 11.84 HH308 pKa = 4.11 VRR310 pKa = 11.84 GHH312 pKa = 5.96 NSPRR316 pKa = 11.84 GRR318 pKa = 11.84 GFYY321 pKa = 10.28 GKK323 pKa = 9.36 PGSPSGAPARR333 pKa = 11.84 SVHH336 pKa = 5.66 EE337 pKa = 4.44 PKK339 pKa = 10.69 PMAATVRR346 pKa = 11.84 SVVQQ350 pKa = 3.29
Molecular weight: 39.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.853
IPC_protein 10.877
Toseland 11.111
ProMoST 11.111
Dawson 11.155
Bjellqvist 10.95
Wikipedia 11.447
Rodwell 11.301
Grimsley 11.199
Solomon 11.418
Lehninger 11.359
Nozaki 11.096
DTASelect 10.95
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.111
Patrickios 11.008
IPC_peptide 11.418
IPC2_peptide 10.16
IPC2.peptide.svr19 8.713
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
10
95
41158
70
2554
433.2
48.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.889 ± 0.237
2.488 ± 0.151
4.84 ± 0.153
5.005 ± 0.133
4.39 ± 0.148
5.919 ± 0.194
2.707 ± 0.094
4.816 ± 0.162
4.087 ± 0.197
10.183 ± 0.249
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.065 ± 0.106
4.019 ± 0.156
6.434 ± 0.284
3.705 ± 0.152
5.979 ± 0.194
7.389 ± 0.181
6.521 ± 0.252
7.199 ± 0.198
1.2 ± 0.083
3.166 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here