Tortoise microvirus 79

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WA22|A0A4P8WA22_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 79 OX=2583186 PE=4 SV=1
MM1 pKa = 7.45TIKK4 pKa = 10.81NLGEE8 pKa = 3.91VFAMRR13 pKa = 11.84RR14 pKa = 11.84LEE16 pKa = 4.3GGSLILPKK24 pKa = 9.07EE25 pKa = 4.1TRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.8VRR31 pKa = 11.84IEE33 pKa = 4.08FNVDD37 pKa = 2.93TPASVYY43 pKa = 10.81AIDD46 pKa = 4.85RR47 pKa = 11.84GQDD50 pKa = 3.38GPGDD54 pKa = 3.73LSSSRR59 pKa = 11.84FIADD63 pKa = 3.32LDD65 pKa = 3.91GFTVLEE71 pKa = 4.86FVAEE75 pKa = 4.81GPLEE79 pKa = 4.03LQVATEE85 pKa = 4.32ANAQVWYY92 pKa = 7.9FTLEE96 pKa = 3.86AEE98 pKa = 4.91QATEE102 pKa = 3.98ANGEE106 pKa = 4.17EE107 pKa = 4.27TQSFAKK113 pKa = 10.41LHH115 pKa = 5.18TPRR118 pKa = 11.84ARR120 pKa = 11.84DD121 pKa = 3.22HH122 pKa = 6.47RR123 pKa = 11.84AEE125 pKa = 3.87MMQFQLQQQQMAFMEE140 pKa = 4.25QMRR143 pKa = 11.84AEE145 pKa = 4.46FDD147 pKa = 3.04RR148 pKa = 11.84RR149 pKa = 11.84LAANPGVHH157 pKa = 6.52PVTGEE162 pKa = 3.84VIEE165 pKa = 4.31DD166 pKa = 3.44VKK168 pKa = 10.97PSSEE172 pKa = 4.07AFGTSGTDD180 pKa = 3.2VSEE183 pKa = 4.42SDD185 pKa = 3.45AVAGADD191 pKa = 3.59EE192 pKa = 4.68PVAEE196 pKa = 4.64PVDD199 pKa = 3.91AKK201 pKa = 10.89AGKK204 pKa = 9.7

Molecular weight: 22.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W7R9|A0A4P8W7R9_9VIRU Uncharacterized protein OS=Tortoise microvirus 79 OX=2583186 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 5.9ASTMARR8 pKa = 11.84RR9 pKa = 11.84SGSRR13 pKa = 11.84RR14 pKa = 11.84EE15 pKa = 3.71SRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84NFLSEE24 pKa = 3.5ASYY27 pKa = 10.7GRR29 pKa = 11.84RR30 pKa = 11.84VAAPQRR36 pKa = 11.84RR37 pKa = 11.84ITTLQPIGRR46 pKa = 11.84PRR48 pKa = 11.84PYY50 pKa = 9.66VQAEE54 pKa = 3.75HH55 pKa = 6.79RR56 pKa = 11.84RR57 pKa = 11.84FGRR60 pKa = 11.84VPQIAPSVISSGQILGGPISAGTGRR85 pKa = 11.84YY86 pKa = 7.37QRR88 pKa = 11.84PEE90 pKa = 3.82EE91 pKa = 4.0PAFLTPNNRR100 pKa = 11.84TTLPDD105 pKa = 3.37NRR107 pKa = 11.84LSSHH111 pKa = 4.65MTRR114 pKa = 11.84NTMCNRR120 pKa = 11.84RR121 pKa = 11.84PDD123 pKa = 3.29SRR125 pKa = 11.84SARR128 pKa = 11.84RR129 pKa = 11.84GNGGSKK135 pKa = 10.26RR136 pKa = 11.84FVPWCC141 pKa = 3.92

Molecular weight: 15.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1889

75

565

236.1

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.688 ± 0.784

0.476 ± 0.349

4.976 ± 0.694

6.935 ± 0.863

3.441 ± 0.349

7.782 ± 1.21

2.912 ± 0.428

4.235 ± 0.453

3.917 ± 1.035

7.835 ± 0.506

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.6 ± 0.294

3.864 ± 1.014

5.717 ± 0.553

4.659 ± 0.41

7.2 ± 1.359

6.458 ± 0.459

4.553 ± 0.55

7.041 ± 0.494

2.065 ± 0.465

2.647 ± 0.757
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski