Methylomicrobium album BG8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomicrobium; Methylomicrobium album

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H8GL03|H8GL03_METAL Anti-anti-sigma regulatory factor (Antagonist of anti-sigma factor) OS=Methylomicrobium album BG8 OX=686340 GN=Metal_2792 PE=4 SV=1
MM1 pKa = 7.8SYY3 pKa = 10.78AQDD6 pKa = 3.29GSGYY10 pKa = 10.11GIYY13 pKa = 9.53GQRR16 pKa = 11.84YY17 pKa = 7.38GANGDD22 pKa = 3.78LQDD25 pKa = 3.48EE26 pKa = 4.53EE27 pKa = 5.27FRR29 pKa = 11.84VNTTTADD36 pKa = 3.6NQVYY40 pKa = 8.83PAVAALNDD48 pKa = 3.69GGYY51 pKa = 10.55LVTWMSYY58 pKa = 10.31AQDD61 pKa = 3.35GSGRR65 pKa = 11.84GIYY68 pKa = 8.05GQRR71 pKa = 11.84YY72 pKa = 8.14GVDD75 pKa = 3.35GTPQGSEE82 pKa = 3.71FRR84 pKa = 11.84VNTTINDD91 pKa = 3.91YY92 pKa = 11.11QNSPAVAALNDD103 pKa = 3.76GGFLVTWMSSGQDD116 pKa = 3.32GSGWGIYY123 pKa = 7.01GQRR126 pKa = 11.84YY127 pKa = 7.98NADD130 pKa = 3.31GDD132 pKa = 3.9PQEE135 pKa = 6.12GEE137 pKa = 4.16FQVNTTTASDD147 pKa = 3.53QQYY150 pKa = 9.47PAVAALNDD158 pKa = 3.76GGFLVTWQSQNQDD171 pKa = 2.45GSGYY175 pKa = 10.21GIYY178 pKa = 9.47GQRR181 pKa = 11.84YY182 pKa = 7.72GADD185 pKa = 3.49GNPQEE190 pKa = 6.3GEE192 pKa = 4.12FQVNTTTASGQWYY205 pKa = 8.36PAVTALNDD213 pKa = 3.5GGYY216 pKa = 10.39LVTWMSDD223 pKa = 3.17GQDD226 pKa = 3.05GSGYY230 pKa = 10.24GIYY233 pKa = 9.52GQRR236 pKa = 11.84YY237 pKa = 8.62DD238 pKa = 4.93ADD240 pKa = 4.02GDD242 pKa = 3.98PLDD245 pKa = 5.52EE246 pKa = 4.36EE247 pKa = 4.72FQVNTTTANQQSNPAVAALDD267 pKa = 3.93DD268 pKa = 4.21GGYY271 pKa = 10.48LVTWMSDD278 pKa = 3.17GQDD281 pKa = 3.05GSGYY285 pKa = 10.24GIYY288 pKa = 9.47GQRR291 pKa = 11.84YY292 pKa = 7.73GADD295 pKa = 3.64GQPVEE300 pKa = 5.08SVVQVTLNHH309 pKa = 6.61APATTPVALDD319 pKa = 3.81AADD322 pKa = 4.02EE323 pKa = 4.58DD324 pKa = 4.81SPGVTITAAQLLAGASDD341 pKa = 4.07EE342 pKa = 5.26DD343 pKa = 5.83DD344 pKa = 4.73GDD346 pKa = 4.03TLQITALSLNDD357 pKa = 3.89PAHH360 pKa = 6.13GQLTAEE366 pKa = 5.03PDD368 pKa = 3.94GTWTFVPAANLNGPVDD384 pKa = 4.13FSYY387 pKa = 10.43TVSDD391 pKa = 3.04GHH393 pKa = 6.94AAFASGSASLTLTPVNDD410 pKa = 4.05APSGTDD416 pKa = 3.0KK417 pKa = 11.01TVTVPEE423 pKa = 5.03DD424 pKa = 3.39GSHH427 pKa = 6.9AFTAADD433 pKa = 4.64FGLTDD438 pKa = 5.3PNDD441 pKa = 3.73TPANSLLAVKK451 pKa = 8.95ITTLPAAGSLTLDD464 pKa = 3.78GVAVTAGQAIVAGDD478 pKa = 4.11LGKK481 pKa = 10.44LAYY484 pKa = 10.17APAANANGAGYY495 pKa = 10.95ASFTFQVQDD504 pKa = 4.41DD505 pKa = 4.26GGTANGGVDD514 pKa = 5.44LDD516 pKa = 4.08GTPNTLTVNVTPVDD530 pKa = 4.15DD531 pKa = 4.91APSLADD537 pKa = 3.32ASRR540 pKa = 11.84SIDD543 pKa = 3.46EE544 pKa = 4.22NSANGTLVYY553 pKa = 10.44DD554 pKa = 4.11VNDD557 pKa = 3.84SVTGTDD563 pKa = 4.22SDD565 pKa = 4.02IDD567 pKa = 4.01SQAITYY573 pKa = 8.92GITGGNSAGGFAIDD587 pKa = 4.28PSTGKK592 pKa = 8.4ITVADD597 pKa = 3.91GTKK600 pKa = 10.33LDD602 pKa = 5.0FEE604 pKa = 4.68TQPSFTLTVTASDD617 pKa = 4.01GALSDD622 pKa = 3.63TATITVNLDD631 pKa = 3.26NLDD634 pKa = 3.36EE635 pKa = 4.23VAPVFTSGTTATAIDD650 pKa = 4.31EE651 pKa = 4.27NSGAGQVVYY660 pKa = 10.84NVVANDD666 pKa = 3.71PATDD670 pKa = 4.28GGPSNPLVYY679 pKa = 10.5SLSGADD685 pKa = 3.64AAALTIDD692 pKa = 3.65SGTGVVTLTGNPNFEE707 pKa = 4.82AKK709 pKa = 9.77PSYY712 pKa = 10.78SFTVVATDD720 pKa = 3.41AAGNAATQAVTLAVNNVDD738 pKa = 3.09EE739 pKa = 4.45VAPVFTSGATAAAIAEE755 pKa = 4.13NSGAGQMVYY764 pKa = 9.27TATTTDD770 pKa = 3.89NPDD773 pKa = 3.04GSHH776 pKa = 7.05PSDD779 pKa = 3.91GPSTRR784 pKa = 11.84TYY786 pKa = 10.96SLSGADD792 pKa = 3.93AAALTINGNTGAVTLTGNPNFEE814 pKa = 4.82AKK816 pKa = 9.77PSYY819 pKa = 10.78SFTVVATDD827 pKa = 3.41AAGNAATQAVTLAINNVDD845 pKa = 3.12EE846 pKa = 4.37VAPVFTSGATATAIAEE862 pKa = 4.09NSGAGQVVYY871 pKa = 8.99TATTTDD877 pKa = 3.89NPDD880 pKa = 3.04GSHH883 pKa = 7.07PSDD886 pKa = 3.85GPSARR891 pKa = 11.84TYY893 pKa = 10.99SLSGADD899 pKa = 3.32AAAFTIDD906 pKa = 3.8SEE908 pKa = 4.58TGEE911 pKa = 4.3VTLTGNPNFEE921 pKa = 4.82AKK923 pKa = 9.77PSYY926 pKa = 10.78SFTVVATDD934 pKa = 3.41AAGNAATQAVTLAINNVDD952 pKa = 3.12EE953 pKa = 4.37VAPVFTSGATATAIAEE969 pKa = 4.09NSGAGQVVYY978 pKa = 8.99TATTTDD984 pKa = 3.89NPDD987 pKa = 3.04GSHH990 pKa = 7.07PSDD993 pKa = 3.85GPSARR998 pKa = 11.84TYY1000 pKa = 10.99SLSGADD1006 pKa = 3.93AAALTINGNTGAVTLTGNPNFEE1028 pKa = 4.57AKK1030 pKa = 9.8PNYY1033 pKa = 9.94SFTVVATDD1041 pKa = 3.41AAGNAATQAVTLAVNNVDD1059 pKa = 3.09EE1060 pKa = 4.45VAPVFTSGATAAAIAEE1076 pKa = 4.14NSGAGQVVYY1085 pKa = 8.99TATTTDD1091 pKa = 3.7NPDD1094 pKa = 3.38AGHH1097 pKa = 7.04PNDD1100 pKa = 4.34GPSARR1105 pKa = 11.84TYY1107 pKa = 10.99SLSGADD1113 pKa = 3.93AAALTINGNTGAVTLTGNPNFEE1135 pKa = 4.57AKK1137 pKa = 9.8PNYY1140 pKa = 9.94SFTVVATDD1148 pKa = 3.41AAGNAATQAVTLAVNNVDD1166 pKa = 3.09EE1167 pKa = 4.45VAPVFTSGATAAAIAEE1183 pKa = 4.13NSGAGQMVYY1192 pKa = 9.27TATTTDD1198 pKa = 3.89NPDD1201 pKa = 3.07GSHH1204 pKa = 7.03PNDD1207 pKa = 3.75GPSARR1212 pKa = 11.84TYY1214 pKa = 11.39SLGGADD1220 pKa = 3.7AAAFTIDD1227 pKa = 3.8SEE1229 pKa = 4.59TGEE1232 pKa = 4.22VTLIGNPDD1240 pKa = 3.57FEE1242 pKa = 4.82TKK1244 pKa = 10.29AGYY1247 pKa = 10.08SFEE1250 pKa = 4.26VTATDD1255 pKa = 3.48AAGNAATQAVTLAVNNLDD1273 pKa = 3.15EE1274 pKa = 4.39VAPVFTSDD1282 pKa = 2.83TTAAAITEE1290 pKa = 4.11NSGAGQVVYY1299 pKa = 8.88TAQATDD1305 pKa = 3.42AGQPSAPLVYY1315 pKa = 10.73SLDD1318 pKa = 3.87GADD1321 pKa = 3.8AAAFSIDD1328 pKa = 3.92EE1329 pKa = 4.2NSGEE1333 pKa = 4.17VTLIDD1338 pKa = 5.28DD1339 pKa = 4.86PDD1341 pKa = 4.17FEE1343 pKa = 5.0TKK1345 pKa = 10.14SSYY1348 pKa = 11.28SFTVAAIDD1356 pKa = 3.72TAGNAATQTVTLAISNVDD1374 pKa = 3.11EE1375 pKa = 4.28VAPVFTSGATAAAIAEE1391 pKa = 4.14NSGAGQVVYY1400 pKa = 8.79TAAANDD1406 pKa = 3.95PATDD1410 pKa = 4.32GGPSNPLVYY1419 pKa = 10.72SLGGADD1425 pKa = 4.29AAALTIDD1432 pKa = 4.0AEE1434 pKa = 4.5TGEE1437 pKa = 4.6VTLTADD1443 pKa = 3.52PNFEE1447 pKa = 3.94IKK1449 pKa = 10.35AGYY1452 pKa = 9.31SFEE1455 pKa = 4.24VTATDD1460 pKa = 3.48AAGNAATQTVTLAVNNVDD1478 pKa = 3.09EE1479 pKa = 4.45VAPVFTSGATATAIAEE1495 pKa = 4.18NSGAAQVVYY1504 pKa = 10.25RR1505 pKa = 11.84AQATDD1510 pKa = 3.92DD1511 pKa = 4.75PDD1513 pKa = 3.93TEE1515 pKa = 4.57HH1516 pKa = 7.24PGDD1519 pKa = 4.25GPSIPLAYY1527 pKa = 10.53SLGGADD1533 pKa = 3.96AAAFSIDD1540 pKa = 3.92EE1541 pKa = 4.2NSGEE1545 pKa = 4.17VTLIDD1550 pKa = 5.28DD1551 pKa = 4.86PDD1553 pKa = 4.17FEE1555 pKa = 5.0TKK1557 pKa = 10.14SSYY1560 pKa = 11.26SFTVVAIDD1568 pKa = 3.56TAGNAATQTVTLAINNLDD1586 pKa = 3.2EE1587 pKa = 4.32VAPVFTSGTTATAIAEE1603 pKa = 4.11NSGAGQVVYY1612 pKa = 8.79TAAANDD1618 pKa = 3.95PATDD1622 pKa = 4.32GGPSNPLVYY1631 pKa = 10.72SLGGADD1637 pKa = 4.29AAALTIDD1644 pKa = 4.0AEE1646 pKa = 4.5TGEE1649 pKa = 4.6VTLTADD1655 pKa = 3.52PNFEE1659 pKa = 3.94IKK1661 pKa = 10.35AGYY1664 pKa = 9.31SFEE1667 pKa = 4.24VTATDD1672 pKa = 3.48AAGNAATQTVTLAVNNVDD1690 pKa = 3.09EE1691 pKa = 4.45VAPVFTSGATATAIAEE1707 pKa = 4.18NSGAAQVVYY1716 pKa = 10.25RR1717 pKa = 11.84AQATDD1722 pKa = 3.92DD1723 pKa = 4.69PDD1725 pKa = 3.92TEE1727 pKa = 4.62HH1728 pKa = 7.26PSDD1731 pKa = 4.39GPSIPLVYY1739 pKa = 10.69SLGGADD1745 pKa = 3.78AAAFSIDD1752 pKa = 3.92EE1753 pKa = 4.2NSGEE1757 pKa = 4.17VTLIDD1762 pKa = 4.6DD1763 pKa = 4.84PNFEE1767 pKa = 4.19AQAGYY1772 pKa = 10.05SFEE1775 pKa = 5.07VIATDD1780 pKa = 3.52TAGNTAIRR1788 pKa = 11.84TVALAIDD1795 pKa = 4.82DD1796 pKa = 4.82LNEE1799 pKa = 4.16APVFTSGTTASVAEE1813 pKa = 4.32NSPPSTVVYY1822 pKa = 8.55TAIASDD1828 pKa = 3.89PDD1830 pKa = 3.37AGQTLTYY1837 pKa = 10.83SLDD1840 pKa = 3.62GSDD1843 pKa = 5.13AGLLEE1848 pKa = 5.02IDD1850 pKa = 4.32AEE1852 pKa = 4.48SGAVTLKK1859 pKa = 9.89TIPDD1863 pKa = 3.76FEE1865 pKa = 4.58TRR1867 pKa = 11.84NSYY1870 pKa = 10.65RR1871 pKa = 11.84FTVIATDD1878 pKa = 3.52NASIALNASQAVILTVTNVPEE1899 pKa = 4.8SDD1901 pKa = 4.15DD1902 pKa = 5.35DD1903 pKa = 4.4NDD1905 pKa = 4.03GVPTEE1910 pKa = 4.19IEE1912 pKa = 3.97MNVPVLADD1920 pKa = 3.42GNGAMVAGDD1929 pKa = 3.83GNGDD1933 pKa = 3.41GVADD1937 pKa = 3.7SRR1939 pKa = 11.84QTNVLSQAVLNTPTAVSHH1957 pKa = 6.93SDD1959 pKa = 3.11GALPVYY1965 pKa = 8.91VTLVADD1971 pKa = 4.17SNAGKK1976 pKa = 10.74ANVTGPSASRR1986 pKa = 11.84ITEE1989 pKa = 4.13FEE1991 pKa = 4.05QLDD1994 pKa = 4.01APADD1998 pKa = 3.95FPDD2001 pKa = 4.38AGAIPLGLFGFSATTQEE2018 pKa = 4.17EE2019 pKa = 5.35GGTEE2023 pKa = 4.2TFSLYY2028 pKa = 10.72LDD2030 pKa = 4.78DD2031 pKa = 6.44DD2032 pKa = 3.89IHH2034 pKa = 8.85VNGYY2038 pKa = 6.82WKK2040 pKa = 10.45KK2041 pKa = 9.98DD2042 pKa = 3.55AQGTWVNLASAAYY2055 pKa = 8.32GGAMTTEE2062 pKa = 4.31GDD2064 pKa = 3.26KK2065 pKa = 10.87TRR2067 pKa = 11.84LDD2069 pKa = 3.78FQITDD2074 pKa = 3.4GGEE2077 pKa = 3.78FDD2079 pKa = 5.47SDD2081 pKa = 3.48GLANGVIRR2089 pKa = 11.84DD2090 pKa = 3.89PGAPAFLPPLGQLINDD2106 pKa = 3.74NNKK2109 pKa = 8.97GNHH2112 pKa = 5.33VYY2114 pKa = 10.95GEE2116 pKa = 3.95NGRR2119 pKa = 11.84NDD2121 pKa = 3.44VLLGNNGNDD2130 pKa = 3.37HH2131 pKa = 6.92LFGGSGDD2138 pKa = 3.27NWLVGGKK2145 pKa = 10.41GKK2147 pKa = 8.92DD2148 pKa = 3.26TYY2150 pKa = 11.03HH2151 pKa = 6.9FSATDD2156 pKa = 3.5RR2157 pKa = 11.84VAGGMDD2163 pKa = 4.97RR2164 pKa = 11.84IVDD2167 pKa = 3.96DD2168 pKa = 4.71NGSLIAFDD2176 pKa = 4.41SALLSQLKK2184 pKa = 10.86LNGTALSQLTGGKK2197 pKa = 9.52NGLGSVIDD2205 pKa = 4.44ANNGLAFKK2213 pKa = 10.81DD2214 pKa = 4.44GILQVDD2220 pKa = 3.72ANGDD2224 pKa = 3.86GVFNADD2230 pKa = 4.39DD2231 pKa = 4.17DD2232 pKa = 4.56FQVRR2236 pKa = 11.84FYY2238 pKa = 11.64NRR2240 pKa = 11.84TADD2243 pKa = 3.6TLRR2246 pKa = 11.84FDD2248 pKa = 3.85TNKK2251 pKa = 10.8DD2252 pKa = 3.5LLILNGKK2259 pKa = 8.22GPKK2262 pKa = 9.47AALPMTGSPQSDD2274 pKa = 3.5TLIGDD2279 pKa = 3.91NKK2281 pKa = 11.12ANDD2284 pKa = 4.34LNGGAADD2291 pKa = 5.25DD2292 pKa = 3.87ILYY2295 pKa = 10.57GAGGRR2300 pKa = 11.84DD2301 pKa = 3.49KK2302 pKa = 11.06LTGGKK2307 pKa = 9.53GADD2310 pKa = 3.58MLVGGAGRR2318 pKa = 11.84DD2319 pKa = 3.53LYY2321 pKa = 11.22YY2322 pKa = 10.19WAAADD2327 pKa = 4.73LEE2329 pKa = 4.89ANGHH2333 pKa = 6.43DD2334 pKa = 4.06RR2335 pKa = 11.84LIDD2338 pKa = 3.87GKK2340 pKa = 10.51GSRR2343 pKa = 11.84LGFDD2347 pKa = 3.58AAILSQLKK2355 pKa = 10.71LGGQALSDD2363 pKa = 3.05ITARR2367 pKa = 11.84KK2368 pKa = 8.16VAIGATLNDD2377 pKa = 4.47DD2378 pKa = 3.48NGVAYY2383 pKa = 10.62RR2384 pKa = 11.84NGVLQMDD2391 pKa = 4.91LNGNGSFDD2399 pKa = 4.78DD2400 pKa = 4.27GDD2402 pKa = 3.86FQIEE2406 pKa = 4.49IVGTTPLKK2414 pKa = 11.09GLVFDD2419 pKa = 5.03GALDD2423 pKa = 3.95VIKK2426 pKa = 10.74LVV2428 pKa = 3.5

Molecular weight:
245.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H8GJ98|H8GJ98_METAL Site-specific recombinase XerD OS=Methylomicrobium album BG8 OX=686340 GN=Metal_1288 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPSKK9 pKa = 10.01LKK11 pKa = 10.39RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.16VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84AKK41 pKa = 10.1LTVV44 pKa = 3.04

Molecular weight:
5.0 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1242637

29

4430

332.4

36.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.2 ± 0.047

1.003 ± 0.016

5.504 ± 0.034

6.205 ± 0.04

4.02 ± 0.027

7.537 ± 0.047

2.295 ± 0.021

5.813 ± 0.034

4.586 ± 0.045

10.811 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.022

3.56 ± 0.028

4.787 ± 0.027

3.936 ± 0.029

6.142 ± 0.047

5.626 ± 0.03

5.033 ± 0.053

6.524 ± 0.032

1.334 ± 0.02

2.86 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski