Amino acid dipepetide frequency for Methylomicrobium album BG8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.983AlaAla: 11.983 ± 0.151
1.07AlaCys: 1.07 ± 0.032
5.8AlaAsp: 5.8 ± 0.082
7.486AlaGlu: 7.486 ± 0.091
3.834AlaPhe: 3.834 ± 0.06
8.592AlaGly: 8.592 ± 0.106
2.087AlaHis: 2.087 ± 0.045
5.664AlaIle: 5.664 ± 0.076
4.424AlaLys: 4.424 ± 0.083
11.282AlaLeu: 11.282 ± 0.13
2.477AlaMet: 2.477 ± 0.046
3.152AlaAsn: 3.152 ± 0.077
4.006AlaPro: 4.006 ± 0.08
3.673AlaGln: 3.673 ± 0.063
5.52AlaArg: 5.52 ± 0.077
5.299AlaSer: 5.299 ± 0.066
4.298AlaThr: 4.298 ± 0.092
7.248AlaVal: 7.248 ± 0.095
1.37AlaTrp: 1.37 ± 0.041
2.732AlaTyr: 2.732 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.942CysAla: 0.942 ± 0.032
0.159CysCys: 0.159 ± 0.014
0.479CysAsp: 0.479 ± 0.024
0.537CysGlu: 0.537 ± 0.026
0.377CysPhe: 0.377 ± 0.018
0.929CysGly: 0.929 ± 0.033
0.328CysHis: 0.328 ± 0.018
0.53CysIle: 0.53 ± 0.022
0.371CysLys: 0.371 ± 0.018
1.12CysLeu: 1.12 ± 0.031
0.198CysMet: 0.198 ± 0.011
0.319CysAsn: 0.319 ± 0.017
0.509CysPro: 0.509 ± 0.022
0.334CysGln: 0.334 ± 0.017
0.806CysArg: 0.806 ± 0.026
0.591CysSer: 0.591 ± 0.023
0.45CysThr: 0.45 ± 0.017
0.606CysVal: 0.606 ± 0.023
0.134CysTrp: 0.134 ± 0.011
0.312CysTyr: 0.312 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.337AspAla: 5.337 ± 0.08
0.587AspCys: 0.587 ± 0.021
2.982AspAsp: 2.982 ± 0.077
3.427AspGlu: 3.427 ± 0.066
2.541AspPhe: 2.541 ± 0.049
4.107AspGly: 4.107 ± 0.114
1.232AspHis: 1.232 ± 0.035
3.418AspIle: 3.418 ± 0.053
2.509AspLys: 2.509 ± 0.05
5.632AspLeu: 5.632 ± 0.085
1.216AspMet: 1.216 ± 0.033
1.844AspAsn: 1.844 ± 0.047
2.836AspPro: 2.836 ± 0.053
1.808AspGln: 1.808 ± 0.04
3.657AspArg: 3.657 ± 0.057
2.904AspSer: 2.904 ± 0.058
2.621AspThr: 2.621 ± 0.058
3.303AspVal: 3.303 ± 0.063
0.987AspTrp: 0.987 ± 0.028
2.087AspTyr: 2.087 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.765GluAla: 6.765 ± 0.073
0.462GluCys: 0.462 ± 0.022
2.729GluAsp: 2.729 ± 0.05
3.531GluGlu: 3.531 ± 0.067
2.248GluPhe: 2.248 ± 0.046
3.738GluGly: 3.738 ± 0.063
1.513GluHis: 1.513 ± 0.039
4.033GluIle: 4.033 ± 0.063
3.575GluLys: 3.575 ± 0.08
6.489GluLeu: 6.489 ± 0.087
1.588GluMet: 1.588 ± 0.038
2.451GluAsn: 2.451 ± 0.053
2.54GluPro: 2.54 ± 0.049
3.182GluGln: 3.182 ± 0.055
4.379GluArg: 4.379 ± 0.066
3.296GluSer: 3.296 ± 0.058
3.517GluThr: 3.517 ± 0.056
3.748GluVal: 3.748 ± 0.057
0.793GluTrp: 0.793 ± 0.024
1.471GluTyr: 1.471 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.812PheAla: 3.812 ± 0.054
0.498PheCys: 0.498 ± 0.021
2.666PheAsp: 2.666 ± 0.052
2.427PheGlu: 2.427 ± 0.045
1.79PhePhe: 1.79 ± 0.049
3.312PheGly: 3.312 ± 0.057
0.828PheHis: 0.828 ± 0.028
2.323PheIle: 2.323 ± 0.049
1.712PheLys: 1.712 ± 0.037
3.82PheLeu: 3.82 ± 0.063
0.824PheMet: 0.824 ± 0.027
1.582PheAsn: 1.582 ± 0.039
1.712PhePro: 1.712 ± 0.037
1.255PheGln: 1.255 ± 0.036
2.193PheArg: 2.193 ± 0.05
2.795PheSer: 2.795 ± 0.054
2.169PheThr: 2.169 ± 0.057
2.57PheVal: 2.57 ± 0.049
0.579PheTrp: 0.579 ± 0.025
1.334PheTyr: 1.334 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
6.415GlyAla: 6.415 ± 0.108
0.928GlyCys: 0.928 ± 0.032
3.877GlyAsp: 3.877 ± 0.081
4.498GlyGlu: 4.498 ± 0.063
3.397GlyPhe: 3.397 ± 0.056
5.765GlyGly: 5.765 ± 0.122
1.707GlyHis: 1.707 ± 0.036
4.886GlyIle: 4.886 ± 0.068
4.154GlyLys: 4.154 ± 0.072
8.004GlyLeu: 8.004 ± 0.105
2.028GlyMet: 2.028 ± 0.046
2.759GlyAsn: 2.759 ± 0.074
2.357GlyPro: 2.357 ± 0.056
2.904GlyGln: 2.904 ± 0.056
4.699GlyArg: 4.699 ± 0.063
4.417GlySer: 4.417 ± 0.08
3.787GlyThr: 3.787 ± 0.112
5.129GlyVal: 5.129 ± 0.084
1.149GlyTrp: 1.149 ± 0.032
2.512GlyTyr: 2.512 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.116HisAla: 2.116 ± 0.048
0.311HisCys: 0.311 ± 0.016
1.27HisAsp: 1.27 ± 0.032
1.24HisGlu: 1.24 ± 0.035
1.127HisPhe: 1.127 ± 0.033
1.811HisGly: 1.811 ± 0.043
0.696HisHis: 0.696 ± 0.031
1.276HisIle: 1.276 ± 0.035
0.808HisLys: 0.808 ± 0.027
2.489HisLeu: 2.489 ± 0.056
0.442HisMet: 0.442 ± 0.019
0.678HisAsn: 0.678 ± 0.023
1.454HisPro: 1.454 ± 0.038
0.905HisGln: 0.905 ± 0.028
1.499HisArg: 1.499 ± 0.035
1.255HisSer: 1.255 ± 0.033
1.013HisThr: 1.013 ± 0.029
1.236HisVal: 1.236 ± 0.035
0.423HisTrp: 0.423 ± 0.019
0.901HisTyr: 0.901 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.513IleAla: 6.513 ± 0.081
0.551IleCys: 0.551 ± 0.022
4.062IleAsp: 4.062 ± 0.076
4.323IleGlu: 4.323 ± 0.074
1.981IlePhe: 1.981 ± 0.045
4.883IleGly: 4.883 ± 0.072
1.309IleHis: 1.309 ± 0.035
2.727IleIle: 2.727 ± 0.062
2.72IleLys: 2.72 ± 0.046
5.293IleLeu: 5.293 ± 0.07
1.019IleMet: 1.019 ± 0.033
2.224IleAsn: 2.224 ± 0.043
2.701IlePro: 2.701 ± 0.05
1.842IleGln: 1.842 ± 0.041
3.615IleArg: 3.615 ± 0.058
3.159IleSer: 3.159 ± 0.055
2.932IleThr: 2.932 ± 0.062
4.124IleVal: 4.124 ± 0.059
0.571IleTrp: 0.571 ± 0.022
1.583IleTyr: 1.583 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
4.749LysAla: 4.749 ± 0.086
0.289LysCys: 0.289 ± 0.014
2.288LysAsp: 2.288 ± 0.055
2.713LysGlu: 2.713 ± 0.055
1.292LysPhe: 1.292 ± 0.037
2.949LysGly: 2.949 ± 0.055
1.084LysHis: 1.084 ± 0.038
3.072LysIle: 3.072 ± 0.053
2.608LysLys: 2.608 ± 0.059
4.657LysLeu: 4.657 ± 0.078
1.114LysMet: 1.114 ± 0.035
1.919LysAsn: 1.919 ± 0.045
2.552LysPro: 2.552 ± 0.052
2.038LysGln: 2.038 ± 0.045
2.74LysArg: 2.74 ± 0.053
2.594LysSer: 2.594 ± 0.062
2.957LysThr: 2.957 ± 0.055
2.805LysVal: 2.805 ± 0.056
0.427LysTrp: 0.427 ± 0.02
1.015LysTyr: 1.015 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
11.925LeuAla: 11.925 ± 0.126
1.094LeuCys: 1.094 ± 0.03
6.154LeuAsp: 6.154 ± 0.092
6.324LeuGlu: 6.324 ± 0.087
4.384LeuPhe: 4.384 ± 0.077
7.297LeuGly: 7.297 ± 0.091
2.36LeuHis: 2.36 ± 0.039
6.067LeuIle: 6.067 ± 0.088
5.322LeuLys: 5.322 ± 0.084
11.66LeuLeu: 11.66 ± 0.162
2.3LeuMet: 2.3 ± 0.052
4.139LeuAsn: 4.139 ± 0.065
5.871LeuPro: 5.871 ± 0.096
4.223LeuGln: 4.223 ± 0.072
6.094LeuArg: 6.094 ± 0.072
6.893LeuSer: 6.893 ± 0.082
5.911LeuThr: 5.911 ± 0.099
6.085LeuVal: 6.085 ± 0.08
1.289LeuTrp: 1.289 ± 0.035
2.722LeuTyr: 2.722 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.418MetAla: 2.418 ± 0.051
0.152MetCys: 0.152 ± 0.01
1.057MetAsp: 1.057 ± 0.032
1.135MetGlu: 1.135 ± 0.038
0.634MetPhe: 0.634 ± 0.028
1.42MetGly: 1.42 ± 0.041
0.474MetHis: 0.474 ± 0.022
1.364MetIle: 1.364 ± 0.032
1.203MetLys: 1.203 ± 0.037
2.547MetLeu: 2.547 ± 0.049
0.58MetMet: 0.58 ± 0.024
0.979MetAsn: 0.979 ± 0.029
1.346MetPro: 1.346 ± 0.039
1.03MetGln: 1.03 ± 0.027
1.379MetArg: 1.379 ± 0.032
1.321MetSer: 1.321 ± 0.034
1.403MetThr: 1.403 ± 0.034
1.306MetVal: 1.306 ± 0.034
0.14MetTrp: 0.14 ± 0.011
0.352MetTyr: 0.352 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.555AsnAla: 3.555 ± 0.055
0.344AsnCys: 0.344 ± 0.019
1.948AsnAsp: 1.948 ± 0.061
1.895AsnGlu: 1.895 ± 0.046
1.309AsnPhe: 1.309 ± 0.036
2.929AsnGly: 2.929 ± 0.078
0.767AsnHis: 0.767 ± 0.026
2.03AsnIle: 2.03 ± 0.048
1.484AsnLys: 1.484 ± 0.037
3.782AsnLeu: 3.782 ± 0.06
0.699AsnMet: 0.699 ± 0.024
1.267AsnAsn: 1.267 ± 0.041
2.382AsnPro: 2.382 ± 0.05
1.386AsnGln: 1.386 ± 0.034
2.482AsnArg: 2.482 ± 0.049
1.765AsnSer: 1.765 ± 0.046
1.77AsnThr: 1.77 ± 0.055
2.206AsnVal: 2.206 ± 0.047
0.497AsnTrp: 0.497 ± 0.021
1.102AsnTyr: 1.102 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.163ProAla: 5.163 ± 0.08
0.436ProCys: 0.436 ± 0.022
3.252ProAsp: 3.252 ± 0.062
4.037ProGlu: 4.037 ± 0.06
1.941ProPhe: 1.941 ± 0.037
3.792ProGly: 3.792 ± 0.07
1.02ProHis: 1.02 ± 0.032
2.356ProIle: 2.356 ± 0.044
1.963ProLys: 1.963 ± 0.043
4.878ProLeu: 4.878 ± 0.076
0.965ProMet: 0.965 ± 0.031
1.614ProAsn: 1.614 ± 0.044
2.298ProPro: 2.298 ± 0.052
1.614ProGln: 1.614 ± 0.043
2.064ProArg: 2.064 ± 0.043
2.603ProSer: 2.603 ± 0.049
2.038ProThr: 2.038 ± 0.047
3.922ProVal: 3.922 ± 0.068
0.582ProTrp: 0.582 ± 0.024
1.329ProTyr: 1.329 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.534GlnAla: 4.534 ± 0.068
0.367GlnCys: 0.367 ± 0.018
1.808GlnAsp: 1.808 ± 0.037
2.174GlnGlu: 2.174 ± 0.039
1.367GlnPhe: 1.367 ± 0.036
2.875GlnGly: 2.875 ± 0.054
0.938GlnHis: 0.938 ± 0.026
2.261GlnIle: 2.261 ± 0.043
1.803GlnLys: 1.803 ± 0.049
4.118GlnLeu: 4.118 ± 0.065
0.864GlnMet: 0.864 ± 0.025
1.409GlnAsn: 1.409 ± 0.037
1.886GlnPro: 1.886 ± 0.042
1.985GlnGln: 1.985 ± 0.057
2.537GlnArg: 2.537 ± 0.051
2.226GlnSer: 2.226 ± 0.045
2.137GlnThr: 2.137 ± 0.043
2.496GlnVal: 2.496 ± 0.046
0.547GlnTrp: 0.547 ± 0.024
1.027GlnTyr: 1.027 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
4.961ArgAla: 4.961 ± 0.075
0.64ArgCys: 0.64 ± 0.024
3.039ArgAsp: 3.039 ± 0.057
3.792ArgGlu: 3.792 ± 0.06
2.978ArgPhe: 2.978 ± 0.043
3.395ArgGly: 3.395 ± 0.06
1.768ArgHis: 1.768 ± 0.049
4.154ArgIle: 4.154 ± 0.064
2.812ArgLys: 2.812 ± 0.061
7.503ArgLeu: 7.503 ± 0.09
1.466ArgMet: 1.466 ± 0.035
2.286ArgAsn: 2.286 ± 0.046
2.698ArgPro: 2.698 ± 0.048
3.128ArgGln: 3.128 ± 0.062
4.171ArgArg: 4.171 ± 0.076
3.114ArgSer: 3.114 ± 0.053
2.792ArgThr: 2.792 ± 0.047
3.592ArgVal: 3.592 ± 0.053
0.932ArgTrp: 0.932 ± 0.03
2.196ArgTyr: 2.196 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
5.711SerAla: 5.711 ± 0.073
0.517SerCys: 0.517 ± 0.022
2.887SerAsp: 2.887 ± 0.056
3.377SerGlu: 3.377 ± 0.056
2.378SerPhe: 2.378 ± 0.054
5.282SerGly: 5.282 ± 0.097
1.253SerHis: 1.253 ± 0.032
3.191SerIle: 3.191 ± 0.052
2.174SerLys: 2.174 ± 0.049
6.349SerLeu: 6.349 ± 0.078
1.24SerMet: 1.24 ± 0.03
1.745SerAsn: 1.745 ± 0.045
2.763SerPro: 2.763 ± 0.051
2.011SerGln: 2.011 ± 0.046
3.506SerArg: 3.506 ± 0.058
3.151SerSer: 3.151 ± 0.065
2.607SerThr: 2.607 ± 0.053
3.816SerVal: 3.816 ± 0.057
0.731SerTrp: 0.731 ± 0.023
1.569SerTyr: 1.569 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
5.336ThrAla: 5.336 ± 0.102
0.422ThrCys: 0.422 ± 0.021
2.77ThrAsp: 2.77 ± 0.063
2.821ThrGlu: 2.821 ± 0.053
1.838ThrPhe: 1.838 ± 0.05
4.86ThrGly: 4.86 ± 0.106
1.21ThrHis: 1.21 ± 0.033
2.696ThrIle: 2.696 ± 0.066
1.503ThrLys: 1.503 ± 0.039
6.239ThrLeu: 6.239 ± 0.113
0.913ThrMet: 0.913 ± 0.031
1.305ThrAsn: 1.305 ± 0.036
2.944ThrPro: 2.944 ± 0.052
1.863ThrGln: 1.863 ± 0.053
2.715ThrArg: 2.715 ± 0.052
2.438ThrSer: 2.438 ± 0.052
2.346ThrThr: 2.346 ± 0.061
4.236ThrVal: 4.236 ± 0.094
0.593ThrTrp: 0.593 ± 0.028
1.282ThrTyr: 1.282 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
6.233ValAla: 6.233 ± 0.083
0.706ValCys: 0.706 ± 0.023
3.747ValAsp: 3.747 ± 0.062
4.127ValGlu: 4.127 ± 0.064
2.884ValPhe: 2.884 ± 0.052
4.42ValGly: 4.42 ± 0.066
1.342ValHis: 1.342 ± 0.035
4.01ValIle: 4.01 ± 0.068
3.117ValLys: 3.117 ± 0.065
7.047ValLeu: 7.047 ± 0.078
1.554ValMet: 1.554 ± 0.036
2.54ValAsn: 2.54 ± 0.069
2.897ValPro: 2.897 ± 0.05
2.118ValGln: 2.118 ± 0.037
3.766ValArg: 3.766 ± 0.055
4.062ValSer: 4.062 ± 0.062
3.568ValThr: 3.568 ± 0.085
4.346ValVal: 4.346 ± 0.071
0.917ValTrp: 0.917 ± 0.03
1.84ValTyr: 1.84 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.963TrpAla: 0.963 ± 0.03
0.132TrpCys: 0.132 ± 0.009
0.646TrpAsp: 0.646 ± 0.025
0.689TrpGlu: 0.689 ± 0.022
0.561TrpPhe: 0.561 ± 0.02
0.756TrpGly: 0.756 ± 0.023
0.347TrpHis: 0.347 ± 0.019
0.795TrpIle: 0.795 ± 0.026
0.554TrpLys: 0.554 ± 0.024
1.909TrpLeu: 1.909 ± 0.047
0.291TrpMet: 0.291 ± 0.016
0.504TrpAsn: 0.504 ± 0.021
0.62TrpPro: 0.62 ± 0.023
0.793TrpGln: 0.793 ± 0.033
1.059TrpArg: 1.059 ± 0.032
0.775TrpSer: 0.775 ± 0.031
0.6TrpThr: 0.6 ± 0.021
0.818TrpVal: 0.818 ± 0.022
0.205TrpTrp: 0.205 ± 0.012
0.318TrpTyr: 0.318 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.051
0.369TyrCys: 0.369 ± 0.017
1.576TyrAsp: 1.576 ± 0.041
1.496TyrGlu: 1.496 ± 0.04
1.34TyrPhe: 1.34 ± 0.036
2.259TyrGly: 2.259 ± 0.053
0.688TyrHis: 0.688 ± 0.023
1.279TyrIle: 1.279 ± 0.03
1.042TyrLys: 1.042 ± 0.036
3.295TyrLeu: 3.295 ± 0.06
0.465TyrMet: 0.465 ± 0.02
0.881TyrAsn: 0.881 ± 0.033
1.494TyrPro: 1.494 ± 0.035
1.27TyrGln: 1.27 ± 0.035
2.534TyrArg: 2.534 ± 0.061
1.6TyrSer: 1.6 ± 0.048
1.416TyrThr: 1.416 ± 0.048
1.646TyrVal: 1.646 ± 0.037
0.467TyrTrp: 0.467 ± 0.021
0.917TyrTyr: 0.917 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3738 proteins (1242637 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski