Pseudoalteromonas phage TW1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5M3Y0|S5M3Y0_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage TW1 OX=1366055 GN=TW1_010 PE=4 SV=1
MM1 pKa = 7.55AFDD4 pKa = 3.92INTVKK9 pKa = 10.69AYY11 pKa = 10.47GGGGSDD17 pKa = 4.7AMMQAEE23 pKa = 3.43IDD25 pKa = 3.67AFNEE29 pKa = 3.89STYY32 pKa = 10.39PCLIASYY39 pKa = 10.48SQLQADD45 pKa = 5.35RR46 pKa = 11.84IANLYY51 pKa = 9.57MSGSLDD57 pKa = 3.29GSEE60 pKa = 4.12ASGAIKK66 pKa = 10.83GEE68 pKa = 4.07TAANGSSVQYY78 pKa = 9.1DD79 pKa = 3.76TTGKK83 pKa = 7.37TQSISNLDD91 pKa = 3.27KK92 pKa = 10.96AYY94 pKa = 10.11EE95 pKa = 4.36ADD97 pKa = 3.65TAGCLPPRR105 pKa = 11.84PIPEE109 pKa = 4.24SPDD112 pKa = 3.22DD113 pKa = 3.72YY114 pKa = 11.24FAFGTAGTSPAADD127 pKa = 3.35NPQQ130 pKa = 2.83

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MS07|S5MS07_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage TW1 OX=1366055 GN=TW1_031 PE=4 SV=1
MM1 pKa = 7.43LRR3 pKa = 11.84QMLNRR8 pKa = 11.84YY9 pKa = 7.09CTEE12 pKa = 3.27NRR14 pKa = 11.84ILKK17 pKa = 9.72KK18 pKa = 10.23QLSNNRR24 pKa = 11.84FLSFVSWIGFCFLVGIQKK42 pKa = 10.69GSFIPVTLFAILVYY56 pKa = 10.8LFLFHH61 pKa = 7.53KK62 pKa = 10.85GKK64 pKa = 10.53LL65 pKa = 3.6

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12056

55

833

194.5

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.349 ± 0.39

1.535 ± 0.19

6.312 ± 0.226

6.586 ± 0.354

4.438 ± 0.184

6.843 ± 0.309

1.609 ± 0.177

6.047 ± 0.209

7.341 ± 0.496

8.096 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.202

6.055 ± 0.278

3.384 ± 0.151

4.521 ± 0.369

4.073 ± 0.24

6.636 ± 0.236

5.549 ± 0.395

6.171 ± 0.274

1.302 ± 0.119

3.683 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski