Amino acid dipepetide frequency for Pseudoalteromonas phage TW1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.139AlaAla: 6.139 ± 0.857
0.912AlaCys: 0.912 ± 0.247
5.143AlaAsp: 5.143 ± 0.764
5.226AlaGlu: 5.226 ± 0.745
3.152AlaPhe: 3.152 ± 0.582
4.645AlaGly: 4.645 ± 0.59
0.995AlaHis: 0.995 ± 0.293
4.479AlaIle: 4.479 ± 0.645
5.724AlaLys: 5.724 ± 0.66
6.885AlaLeu: 6.885 ± 0.875
1.991AlaMet: 1.991 ± 0.416
4.728AlaAsn: 4.728 ± 0.58
2.489AlaPro: 2.489 ± 0.33
4.148AlaGln: 4.148 ± 0.662
2.323AlaArg: 2.323 ± 0.4
3.484AlaSer: 3.484 ± 0.503
2.737AlaThr: 2.737 ± 0.467
4.894AlaVal: 4.894 ± 0.497
0.83AlaTrp: 0.83 ± 0.298
2.572AlaTyr: 2.572 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
0.747CysAla: 0.747 ± 0.244
0.332CysCys: 0.332 ± 0.157
1.327CysAsp: 1.327 ± 0.381
1.327CysGlu: 1.327 ± 0.388
0.912CysPhe: 0.912 ± 0.299
1.078CysGly: 1.078 ± 0.364
0.332CysHis: 0.332 ± 0.147
0.83CysIle: 0.83 ± 0.23
1.161CysLys: 1.161 ± 0.364
1.327CysLeu: 1.327 ± 0.379
0.249CysMet: 0.249 ± 0.151
0.83CysAsn: 0.83 ± 0.284
0.249CysPro: 0.249 ± 0.126
0.249CysGln: 0.249 ± 0.127
0.664CysArg: 0.664 ± 0.223
1.161CysSer: 1.161 ± 0.362
0.995CysThr: 0.995 ± 0.292
0.912CysVal: 0.912 ± 0.271
0.166CysTrp: 0.166 ± 0.117
0.498CysTyr: 0.498 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
4.314AspAla: 4.314 ± 0.547
0.83AspCys: 0.83 ± 0.293
3.899AspAsp: 3.899 ± 0.55
4.397AspGlu: 4.397 ± 0.541
2.986AspPhe: 2.986 ± 0.5
4.894AspGly: 4.894 ± 0.728
0.83AspHis: 0.83 ± 0.254
4.231AspIle: 4.231 ± 0.449
4.231AspLys: 4.231 ± 0.661
5.724AspLeu: 5.724 ± 0.653
0.995AspMet: 0.995 ± 0.295
3.152AspAsn: 3.152 ± 0.456
2.323AspPro: 2.323 ± 0.454
2.157AspGln: 2.157 ± 0.374
2.157AspArg: 2.157 ± 0.366
4.811AspSer: 4.811 ± 0.585
3.65AspThr: 3.65 ± 0.544
3.816AspVal: 3.816 ± 0.607
0.912AspTrp: 0.912 ± 0.349
2.82AspTyr: 2.82 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
4.645GluAla: 4.645 ± 0.64
1.078GluCys: 1.078 ± 0.327
2.406GluAsp: 2.406 ± 0.429
3.069GluGlu: 3.069 ± 0.584
3.899GluPhe: 3.899 ± 0.594
3.152GluGly: 3.152 ± 0.433
1.161GluHis: 1.161 ± 0.367
4.645GluIle: 4.645 ± 0.56
4.811GluLys: 4.811 ± 0.613
6.304GluLeu: 6.304 ± 0.66
2.074GluMet: 2.074 ± 0.376
3.65GluAsn: 3.65 ± 0.452
1.908GluPro: 1.908 ± 0.377
4.065GluGln: 4.065 ± 0.562
3.235GluArg: 3.235 ± 0.446
4.811GluSer: 4.811 ± 0.582
2.737GluThr: 2.737 ± 0.481
5.06GluVal: 5.06 ± 0.598
0.664GluTrp: 0.664 ± 0.256
2.406GluTyr: 2.406 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
3.899PheAla: 3.899 ± 0.67
0.664PheCys: 0.664 ± 0.224
3.069PheAsp: 3.069 ± 0.546
3.401PheGlu: 3.401 ± 0.536
1.493PhePhe: 1.493 ± 0.348
4.148PheGly: 4.148 ± 0.58
0.498PheHis: 0.498 ± 0.186
3.152PheIle: 3.152 ± 0.482
3.816PheLys: 3.816 ± 0.651
2.82PheLeu: 2.82 ± 0.555
0.912PheMet: 0.912 ± 0.315
4.314PheAsn: 4.314 ± 0.556
0.415PhePro: 0.415 ± 0.148
1.078PheGln: 1.078 ± 0.328
1.41PheArg: 1.41 ± 0.367
2.489PheSer: 2.489 ± 0.535
1.742PheThr: 1.742 ± 0.378
2.24PheVal: 2.24 ± 0.461
0.912PheTrp: 0.912 ± 0.232
1.908PheTyr: 1.908 ± 0.401
0.0PheXaa: 0.0 ± 0.0
Gly
4.894GlyAla: 4.894 ± 0.595
1.742GlyCys: 1.742 ± 0.376
5.06GlyAsp: 5.06 ± 0.557
4.728GlyGlu: 4.728 ± 0.662
4.397GlyPhe: 4.397 ± 0.682
5.226GlyGly: 5.226 ± 0.727
0.83GlyHis: 0.83 ± 0.279
3.484GlyIle: 3.484 ± 0.521
5.309GlyLys: 5.309 ± 0.759
5.06GlyLeu: 5.06 ± 0.656
1.327GlyMet: 1.327 ± 0.318
3.069GlyAsn: 3.069 ± 0.489
1.244GlyPro: 1.244 ± 0.326
2.489GlyGln: 2.489 ± 0.562
2.986GlyArg: 2.986 ± 0.467
3.982GlySer: 3.982 ± 0.608
3.484GlyThr: 3.484 ± 0.676
5.558GlyVal: 5.558 ± 0.606
0.747GlyTrp: 0.747 ± 0.22
2.82GlyTyr: 2.82 ± 0.532
0.0GlyXaa: 0.0 ± 0.0
His
1.659HisAla: 1.659 ± 0.5
0.249HisCys: 0.249 ± 0.133
0.912HisAsp: 0.912 ± 0.446
0.581HisGlu: 0.581 ± 0.188
1.161HisPhe: 1.161 ± 0.267
0.498HisGly: 0.498 ± 0.174
0.332HisHis: 0.332 ± 0.178
1.493HisIle: 1.493 ± 0.294
1.078HisLys: 1.078 ± 0.352
1.244HisLeu: 1.244 ± 0.352
0.581HisMet: 0.581 ± 0.211
1.493HisAsn: 1.493 ± 0.412
0.581HisPro: 0.581 ± 0.212
0.581HisGln: 0.581 ± 0.318
0.581HisArg: 0.581 ± 0.197
0.747HisSer: 0.747 ± 0.251
0.498HisThr: 0.498 ± 0.209
0.747HisVal: 0.747 ± 0.225
0.249HisTrp: 0.249 ± 0.152
0.83HisTyr: 0.83 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
5.06IleAla: 5.06 ± 0.803
0.664IleCys: 0.664 ± 0.206
5.392IleAsp: 5.392 ± 0.685
5.558IleGlu: 5.558 ± 0.594
1.991IlePhe: 1.991 ± 0.429
4.148IleGly: 4.148 ± 0.602
1.161IleHis: 1.161 ± 0.295
4.314IleIle: 4.314 ± 0.641
4.811IleLys: 4.811 ± 0.58
2.82IleLeu: 2.82 ± 0.435
1.576IleMet: 1.576 ± 0.352
4.397IleAsn: 4.397 ± 0.549
2.737IlePro: 2.737 ± 0.373
1.742IleGln: 1.742 ± 0.464
1.991IleArg: 1.991 ± 0.371
3.484IleSer: 3.484 ± 0.517
3.982IleThr: 3.982 ± 0.413
2.24IleVal: 2.24 ± 0.424
0.581IleTrp: 0.581 ± 0.257
1.825IleTyr: 1.825 ± 0.445
0.0IleXaa: 0.0 ± 0.0
Lys
5.06LysAla: 5.06 ± 0.714
0.83LysCys: 0.83 ± 0.288
3.235LysAsp: 3.235 ± 0.572
5.724LysGlu: 5.724 ± 0.751
1.991LysPhe: 1.991 ± 0.446
4.314LysGly: 4.314 ± 0.614
1.493LysHis: 1.493 ± 0.395
4.065LysIle: 4.065 ± 0.671
4.894LysLys: 4.894 ± 0.681
7.383LysLeu: 7.383 ± 0.839
1.576LysMet: 1.576 ± 0.403
3.733LysAsn: 3.733 ± 0.592
3.733LysPro: 3.733 ± 0.691
3.567LysGln: 3.567 ± 0.514
2.737LysArg: 2.737 ± 0.556
7.051LysSer: 7.051 ± 0.675
3.401LysThr: 3.401 ± 0.446
4.562LysVal: 4.562 ± 0.762
1.659LysTrp: 1.659 ± 0.31
2.406LysTyr: 2.406 ± 0.384
0.0LysXaa: 0.0 ± 0.0
Leu
7.3LeuAla: 7.3 ± 0.778
1.493LeuCys: 1.493 ± 0.322
5.226LeuAsp: 5.226 ± 0.676
4.231LeuGlu: 4.231 ± 0.517
2.489LeuPhe: 2.489 ± 0.436
4.894LeuGly: 4.894 ± 0.601
2.24LeuHis: 2.24 ± 0.478
5.226LeuIle: 5.226 ± 0.646
6.387LeuLys: 6.387 ± 0.598
6.304LeuLeu: 6.304 ± 0.865
1.991LeuMet: 1.991 ± 0.374
4.894LeuAsn: 4.894 ± 0.579
2.986LeuPro: 2.986 ± 0.555
2.24LeuGln: 2.24 ± 0.386
2.903LeuArg: 2.903 ± 0.498
5.309LeuSer: 5.309 ± 0.68
6.885LeuThr: 6.885 ± 0.701
4.397LeuVal: 4.397 ± 0.511
0.83LeuTrp: 0.83 ± 0.227
2.737LeuTyr: 2.737 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
2.074MetAla: 2.074 ± 0.414
0.166MetCys: 0.166 ± 0.112
1.078MetAsp: 1.078 ± 0.294
0.995MetGlu: 0.995 ± 0.251
0.995MetPhe: 0.995 ± 0.288
1.078MetGly: 1.078 ± 0.342
0.166MetHis: 0.166 ± 0.115
1.576MetIle: 1.576 ± 0.418
2.157MetLys: 2.157 ± 0.612
2.24MetLeu: 2.24 ± 0.402
0.83MetMet: 0.83 ± 0.286
1.41MetAsn: 1.41 ± 0.429
1.078MetPro: 1.078 ± 0.309
1.244MetGln: 1.244 ± 0.353
1.659MetArg: 1.659 ± 0.347
2.655MetSer: 2.655 ± 0.547
1.078MetThr: 1.078 ± 0.266
1.244MetVal: 1.244 ± 0.384
0.332MetTrp: 0.332 ± 0.159
0.664MetTyr: 0.664 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.733AsnAla: 3.733 ± 0.476
0.995AsnCys: 0.995 ± 0.276
3.65AsnAsp: 3.65 ± 0.547
3.567AsnGlu: 3.567 ± 0.522
2.157AsnPhe: 2.157 ± 0.331
5.475AsnGly: 5.475 ± 0.763
0.415AsnHis: 0.415 ± 0.18
2.24AsnIle: 2.24 ± 0.318
4.811AsnLys: 4.811 ± 0.472
4.479AsnLeu: 4.479 ± 0.546
1.825AsnMet: 1.825 ± 0.395
3.899AsnAsn: 3.899 ± 0.473
3.567AsnPro: 3.567 ± 0.548
2.903AsnGln: 2.903 ± 0.49
3.069AsnArg: 3.069 ± 0.542
3.899AsnSer: 3.899 ± 0.884
3.65AsnThr: 3.65 ± 0.584
3.318AsnVal: 3.318 ± 0.592
0.83AsnTrp: 0.83 ± 0.231
2.074AsnTyr: 2.074 ± 0.484
0.0AsnXaa: 0.0 ± 0.0
Pro
2.572ProAla: 2.572 ± 0.463
0.332ProCys: 0.332 ± 0.155
1.825ProAsp: 1.825 ± 0.373
2.489ProGlu: 2.489 ± 0.386
1.825ProPhe: 1.825 ± 0.336
0.747ProGly: 0.747 ± 0.216
0.332ProHis: 0.332 ± 0.182
2.157ProIle: 2.157 ± 0.335
2.903ProLys: 2.903 ± 0.563
2.82ProLeu: 2.82 ± 0.532
0.83ProMet: 0.83 ± 0.289
1.659ProAsn: 1.659 ± 0.349
1.742ProPro: 1.742 ± 0.311
1.908ProGln: 1.908 ± 0.417
0.912ProArg: 0.912 ± 0.295
2.406ProSer: 2.406 ± 0.406
2.074ProThr: 2.074 ± 0.342
2.82ProVal: 2.82 ± 0.505
0.581ProTrp: 0.581 ± 0.182
0.912ProTyr: 0.912 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
3.567GlnAla: 3.567 ± 0.704
0.415GlnCys: 0.415 ± 0.196
2.655GlnAsp: 2.655 ± 0.495
2.82GlnGlu: 2.82 ± 0.474
2.074GlnPhe: 2.074 ± 0.385
2.986GlnGly: 2.986 ± 0.567
0.664GlnHis: 0.664 ± 0.206
2.986GlnIle: 2.986 ± 0.568
2.82GlnLys: 2.82 ± 0.509
3.484GlnLeu: 3.484 ± 0.562
1.244GlnMet: 1.244 ± 0.272
2.406GlnAsn: 2.406 ± 0.472
1.41GlnPro: 1.41 ± 0.386
3.484GlnGln: 3.484 ± 1.444
1.576GlnArg: 1.576 ± 0.336
2.986GlnSer: 2.986 ± 0.507
3.567GlnThr: 3.567 ± 0.689
2.406GlnVal: 2.406 ± 0.471
0.498GlnTrp: 0.498 ± 0.176
1.161GlnTyr: 1.161 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
2.986ArgAla: 2.986 ± 0.413
0.498ArgCys: 0.498 ± 0.176
3.152ArgAsp: 3.152 ± 0.454
2.074ArgGlu: 2.074 ± 0.42
1.659ArgPhe: 1.659 ± 0.377
2.903ArgGly: 2.903 ± 0.509
0.581ArgHis: 0.581 ± 0.22
2.737ArgIle: 2.737 ± 0.375
2.82ArgLys: 2.82 ± 0.547
3.401ArgLeu: 3.401 ± 0.436
0.83ArgMet: 0.83 ± 0.268
1.493ArgAsn: 1.493 ± 0.406
0.747ArgPro: 0.747 ± 0.24
2.572ArgGln: 2.572 ± 0.382
1.244ArgArg: 1.244 ± 0.295
2.406ArgSer: 2.406 ± 0.526
1.825ArgThr: 1.825 ± 0.358
3.484ArgVal: 3.484 ± 0.535
0.83ArgTrp: 0.83 ± 0.284
2.489ArgTyr: 2.489 ± 0.447
0.0ArgXaa: 0.0 ± 0.0
Ser
4.231SerAla: 4.231 ± 0.664
0.995SerCys: 0.995 ± 0.34
4.562SerAsp: 4.562 ± 0.578
4.811SerGlu: 4.811 ± 0.552
3.318SerPhe: 3.318 ± 0.495
5.392SerGly: 5.392 ± 0.72
0.995SerHis: 0.995 ± 0.315
4.231SerIle: 4.231 ± 0.455
4.728SerLys: 4.728 ± 0.665
3.982SerLeu: 3.982 ± 0.615
1.908SerMet: 1.908 ± 0.389
4.479SerAsn: 4.479 ± 0.349
1.991SerPro: 1.991 ± 0.437
2.903SerGln: 2.903 ± 0.44
2.82SerArg: 2.82 ± 0.651
3.982SerSer: 3.982 ± 0.506
3.235SerThr: 3.235 ± 0.501
4.894SerVal: 4.894 ± 0.624
0.747SerTrp: 0.747 ± 0.25
2.157SerTyr: 2.157 ± 0.381
0.0SerXaa: 0.0 ± 0.0
Thr
4.231ThrAla: 4.231 ± 0.495
0.747ThrCys: 0.747 ± 0.248
3.899ThrAsp: 3.899 ± 0.563
3.733ThrGlu: 3.733 ± 0.717
3.401ThrPhe: 3.401 ± 0.657
3.318ThrGly: 3.318 ± 0.37
1.078ThrHis: 1.078 ± 0.332
2.986ThrIle: 2.986 ± 0.565
3.318ThrLys: 3.318 ± 0.545
5.143ThrLeu: 5.143 ± 0.595
0.995ThrMet: 0.995 ± 0.275
3.733ThrAsn: 3.733 ± 0.588
1.825ThrPro: 1.825 ± 0.343
2.82ThrGln: 2.82 ± 0.547
2.157ThrArg: 2.157 ± 0.49
2.655ThrSer: 2.655 ± 0.361
3.069ThrThr: 3.069 ± 0.474
3.235ThrVal: 3.235 ± 0.525
0.415ThrTrp: 0.415 ± 0.161
2.737ThrTyr: 2.737 ± 0.56
0.0ThrXaa: 0.0 ± 0.0
Val
4.065ValAla: 4.065 ± 0.419
1.078ValCys: 1.078 ± 0.347
3.982ValAsp: 3.982 ± 0.715
3.899ValGlu: 3.899 ± 0.455
2.323ValPhe: 2.323 ± 0.456
5.226ValGly: 5.226 ± 0.667
0.664ValHis: 0.664 ± 0.257
3.152ValIle: 3.152 ± 0.449
4.314ValLys: 4.314 ± 0.572
5.06ValLeu: 5.06 ± 0.575
2.24ValMet: 2.24 ± 0.435
4.894ValAsn: 4.894 ± 0.724
1.244ValPro: 1.244 ± 0.296
2.157ValGln: 2.157 ± 0.409
3.152ValArg: 3.152 ± 0.522
4.811ValSer: 4.811 ± 0.726
3.982ValThr: 3.982 ± 0.731
3.069ValVal: 3.069 ± 0.5
0.664ValTrp: 0.664 ± 0.237
1.742ValTyr: 1.742 ± 0.392
0.0ValXaa: 0.0 ± 0.0
Trp
0.664TrpAla: 0.664 ± 0.252
0.415TrpCys: 0.415 ± 0.192
0.415TrpAsp: 0.415 ± 0.184
0.332TrpGlu: 0.332 ± 0.156
0.747TrpPhe: 0.747 ± 0.272
0.912TrpGly: 0.912 ± 0.273
0.664TrpHis: 0.664 ± 0.219
0.498TrpIle: 0.498 ± 0.212
0.995TrpLys: 0.995 ± 0.322
1.244TrpLeu: 1.244 ± 0.36
0.083TrpMet: 0.083 ± 0.08
0.664TrpAsn: 0.664 ± 0.286
0.581TrpPro: 0.581 ± 0.219
0.747TrpGln: 0.747 ± 0.256
0.995TrpArg: 0.995 ± 0.316
0.747TrpSer: 0.747 ± 0.231
0.664TrpThr: 0.664 ± 0.233
0.664TrpVal: 0.664 ± 0.211
0.166TrpTrp: 0.166 ± 0.137
0.83TrpTyr: 0.83 ± 0.27
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.659TyrAla: 1.659 ± 0.337
0.912TyrCys: 0.912 ± 0.312
2.24TyrAsp: 2.24 ± 0.509
2.903TyrGlu: 2.903 ± 0.385
1.41TyrPhe: 1.41 ± 0.371
3.401TyrGly: 3.401 ± 0.462
0.664TyrHis: 0.664 ± 0.24
1.991TyrIle: 1.991 ± 0.363
2.323TyrLys: 2.323 ± 0.383
3.235TyrLeu: 3.235 ± 0.514
0.581TyrMet: 0.581 ± 0.21
1.659TyrAsn: 1.659 ± 0.348
0.995TyrPro: 0.995 ± 0.264
2.157TyrGln: 2.157 ± 0.487
2.157TyrArg: 2.157 ± 0.37
2.489TyrSer: 2.489 ± 0.538
2.24TyrThr: 2.24 ± 0.465
2.157TyrVal: 2.157 ± 0.461
0.415TyrTrp: 0.415 ± 0.195
1.244TyrTyr: 1.244 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12056 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski