Pseudomonas phage tabernarius
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4P7B8|A0A2H4P7B8_9CAUD Uncharacterized protein OS=Pseudomonas phage tabernarius OX=2048978 GN=CNR33_00084 PE=4 SV=1
MM1 pKa = 7.39 SNAYY5 pKa = 9.72 NADD8 pKa = 2.89 GRR10 pKa = 11.84 EE11 pKa = 3.81 PSEE14 pKa = 4.91 DD15 pKa = 3.6 YY16 pKa = 10.5 IKK18 pKa = 11.51 AMMYY22 pKa = 10.23 VIEE25 pKa = 4.38 EE26 pKa = 4.38 HH27 pKa = 5.66 TTDD30 pKa = 4.49 LSGQEE35 pKa = 4.49 IYY37 pKa = 10.68 DD38 pKa = 3.58 ITHH41 pKa = 5.95 TLIMSGFYY49 pKa = 10.08 TGIEE53 pKa = 3.81 RR54 pKa = 11.84 HH55 pKa = 5.14 QLEE58 pKa = 4.32 GHH60 pKa = 6.78 LVPEE64 pKa = 4.8 GSVEE68 pKa = 4.07 KK69 pKa = 10.95 LRR71 pKa = 11.84 GTVNSYY77 pKa = 10.41 PPRR80 pKa = 11.84 EE81 pKa = 4.02 PVLKK85 pKa = 10.06 TEE87 pKa = 4.13 NQYY90 pKa = 10.86 EE91 pKa = 4.18 LEE93 pKa = 4.17 LTKK96 pKa = 10.7 EE97 pKa = 3.95 LGGAIDD103 pKa = 4.48 GPFSEE108 pKa = 5.19 ALEE111 pKa = 4.17 NAKK114 pKa = 10.42 KK115 pKa = 9.74 EE116 pKa = 4.12 ASEE119 pKa = 4.24 VEE121 pKa = 3.84 PDD123 pKa = 3.56 LAAGRR128 pKa = 11.84 ASYY131 pKa = 7.96 GTSEE135 pKa = 4.56 CTCDD139 pKa = 3.64 QCTPYY144 pKa = 10.96 NWDD147 pKa = 3.08
Molecular weight: 16.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.115
IPC2_protein 4.418
IPC_protein 4.317
Toseland 4.177
ProMoST 4.355
Dawson 4.253
Bjellqvist 4.431
Wikipedia 4.101
Rodwell 4.164
Grimsley 4.088
Solomon 4.253
Lehninger 4.202
Nozaki 4.368
DTASelect 4.457
Thurlkill 4.177
EMBOSS 4.113
Sillero 4.431
Patrickios 1.964
IPC_peptide 4.253
IPC2_peptide 4.418
IPC2.peptide.svr19 4.349
Protein with the highest isoelectric point:
>tr|A0A2H4P6P8|A0A2H4P6P8_9CAUD Uncharacterized protein OS=Pseudomonas phage tabernarius OX=2048978 GN=CNR33_00009 PE=4 SV=1
MM1 pKa = 7.22 KK2 pKa = 10.51 QPTFPANEE10 pKa = 4.19 LDD12 pKa = 4.23 CLFLLAVMLMSHH24 pKa = 6.95 RR25 pKa = 11.84 NNPAKK30 pKa = 10.21 LRR32 pKa = 11.84 HH33 pKa = 4.59 VAKK36 pKa = 10.38 LQVKK40 pKa = 8.61 NVPRR44 pKa = 11.84 STRR47 pKa = 11.84 PAFAMIALSKK57 pKa = 10.96 NPAAVIQSAIEE68 pKa = 3.95 NFF70 pKa = 3.56
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.237
IPC2_protein 9.545
IPC_protein 9.75
Toseland 10.906
ProMoST 10.394
Dawson 10.95
Bjellqvist 10.54
Wikipedia 11.067
Rodwell 11.403
Grimsley 10.95
Solomon 11.052
Lehninger 11.038
Nozaki 10.877
DTASelect 10.54
Thurlkill 10.862
EMBOSS 11.286
Sillero 10.877
Patrickios 11.199
IPC_peptide 11.067
IPC2_peptide 9.268
IPC2.peptide.svr19 8.648
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
20365
44
1014
228.8
25.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.718 ± 0.324
1.036 ± 0.107
5.991 ± 0.225
6.118 ± 0.421
4.297 ± 0.182
7.596 ± 0.373
1.836 ± 0.171
5.166 ± 0.186
5.838 ± 0.408
7.508 ± 0.206
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.877 ± 0.145
4.719 ± 0.301
4.346 ± 0.19
3.82 ± 0.204
4.866 ± 0.26
6.752 ± 0.329
5.922 ± 0.251
7.258 ± 0.248
1.326 ± 0.101
3.01 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here