Fontibacillus phaseoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Fontibacillus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369B6C8|A0A369B6C8_9BACL GntR family transcriptional regulator OS=Fontibacillus phaseoli OX=1416533 GN=DFP94_110132 PE=4 SV=1
MM1 pKa = 7.51PVIKK5 pKa = 10.04PVFTAVSTAPVATGGVITTAITPDD29 pKa = 3.03TSRR32 pKa = 11.84YY33 pKa = 9.24FVTLTAGMIGATEE46 pKa = 4.16TTIPAASFVDD56 pKa = 4.1DD57 pKa = 4.35ANGPVAALPTLAASDD72 pKa = 3.98YY73 pKa = 10.48FNVYY77 pKa = 10.04INGVLQQASLSTLTTASLVLDD98 pKa = 4.27TVDD101 pKa = 3.79LTAGIPIQLEE111 pKa = 4.15VASFSTVASNITTQPTISAPTITIITT137 pKa = 3.92

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369AWR1|A0A369AWR1_9BACL Fructokinase OS=Fontibacillus phaseoli OX=1416533 GN=DFP94_1209 PE=4 SV=1
MM1 pKa = 7.49LVNLLSSRR9 pKa = 11.84TKK11 pKa = 9.79AYY13 pKa = 10.7LFRR16 pKa = 11.84RR17 pKa = 11.84SCLKK21 pKa = 10.56ASLIIAGSVIQGFGMGVFLFPHH43 pKa = 7.64AIPPGGAGGLALLLNHH59 pKa = 7.03WFSFPMSIGFWLMNFLFLLTAIPFLGTVSTVGTIGVITTTSVSVNIFGVYY109 pKa = 10.05FNSPLGNVWFDD120 pKa = 3.74LLAGSVLLGIGIAVLLTQRR139 pKa = 11.84FSNGGIGFAALALSRR154 pKa = 11.84QKK156 pKa = 10.92HH157 pKa = 5.1FDD159 pKa = 3.33PGKK162 pKa = 8.34TLFWMNGAIFILTACVIDD180 pKa = 3.36WMIIIQALLCQWISTSIVSWLYY202 pKa = 9.28STSFPARR209 pKa = 11.84PLYY212 pKa = 10.41AWRR215 pKa = 11.84GKK217 pKa = 9.92RR218 pKa = 11.84DD219 pKa = 3.16RR220 pKa = 4.51

Molecular weight: 24.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4966

0

4966

1564184

29

2503

315.0

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.975 ± 0.04

0.785 ± 0.01

5.161 ± 0.026

6.996 ± 0.036

4.215 ± 0.026

7.744 ± 0.038

2.011 ± 0.019

6.7 ± 0.031

5.453 ± 0.026

10.197 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.016

3.866 ± 0.029

3.998 ± 0.023

3.658 ± 0.019

5.053 ± 0.028

6.383 ± 0.026

5.211 ± 0.024

7.044 ± 0.031

1.251 ± 0.017

3.503 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski