Arthrobotrys flagrans
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10325 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437A4J4|A0A437A4J4_9PEZI Uncharacterized protein OS=Arthrobotrys flagrans OX=97331 GN=DFL_004315 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 FSTLAFAAIAVLPSAISAFSLYY24 pKa = 10.49 DD25 pKa = 3.29 ISSDD29 pKa = 4.89 LGDD32 pKa = 5.19 ANDD35 pKa = 4.09 KK36 pKa = 10.77 CASQYY41 pKa = 10.89 SIPLMSCGFLPGKK54 pKa = 10.49 CSDD57 pKa = 3.46 ACQEE61 pKa = 4.04 QLQSLEE67 pKa = 4.05 TTLQGVCKK75 pKa = 10.27 DD76 pKa = 3.44 SKK78 pKa = 11.06 KK79 pKa = 10.29 PQNLLLAALNDD90 pKa = 3.86 GLVPALCVNAASSGSSSSSGSDD112 pKa = 3.32 DD113 pKa = 3.82 TPSATPTFMSGPGKK127 pKa = 8.92 TPSSSAAQLTFSGVVEE143 pKa = 4.2 ATSATEE149 pKa = 4.11 DD150 pKa = 3.42 QSEE153 pKa = 4.31 PTGLVIDD160 pKa = 4.08 TSVPEE165 pKa = 4.13 TTATQTVAYY174 pKa = 8.89 PEE176 pKa = 3.96 NTYY179 pKa = 11.33 APTTLQTSNSPASTTSSSSEE199 pKa = 3.65 GSGNGGSNGSPFSSQDD215 pKa = 3.39 DD216 pKa = 4.12 NPNSGAAVRR225 pKa = 11.84 VSAFGVAIGVFALIANIAA243 pKa = 3.37
Molecular weight: 24.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.973
Patrickios 0.947
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A436ZY54|A0A436ZY54_9PEZI Uncharacterized protein OS=Arthrobotrys flagrans OX=97331 GN=DFL_005670 PE=4 SV=1
MM1 pKa = 7.29 TVLTLLRR8 pKa = 11.84 RR9 pKa = 11.84 PAVSCLRR16 pKa = 11.84 SSSSLLSSTSRR27 pKa = 11.84 RR28 pKa = 11.84 TFTSLLRR35 pKa = 11.84 TPTALATLQTRR46 pKa = 11.84 PTPSTPTPLLPQTISPLLPSASTSLQGLVQTRR78 pKa = 11.84 GHH80 pKa = 6.2 RR81 pKa = 11.84 RR82 pKa = 11.84 KK83 pKa = 8.38 TYY85 pKa = 8.98 NPSHH89 pKa = 7.03 IVRR92 pKa = 11.84 KK93 pKa = 9.74 RR94 pKa = 11.84 RR95 pKa = 11.84 FGFLARR101 pKa = 11.84 LRR103 pKa = 11.84 SKK105 pKa = 9.3 TGKK108 pKa = 10.27 NILKK112 pKa = 9.96 RR113 pKa = 11.84 RR114 pKa = 11.84 KK115 pKa = 9.1 AKK117 pKa = 8.97 GRR119 pKa = 11.84 KK120 pKa = 8.03 MLTHH124 pKa = 7.27
Molecular weight: 13.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.082
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9880
445
10325
5006540
66
12066
484.9
53.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.793 ± 0.022
1.157 ± 0.01
5.594 ± 0.016
6.648 ± 0.03
3.679 ± 0.015
6.864 ± 0.023
2.2 ± 0.011
5.275 ± 0.016
5.602 ± 0.025
8.489 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.009
3.979 ± 0.012
6.282 ± 0.035
3.789 ± 0.02
5.866 ± 0.022
8.247 ± 0.027
6.329 ± 0.02
5.979 ± 0.019
1.321 ± 0.009
2.868 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here