Pseudoduganella sp. CY13W

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Pseudoduganella; unclassified Pseudoduganella

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L8L324|A0A6L8L324_9BURK PEP-CTERM sorting domain-containing protein OS=Pseudoduganella sp. CY13W OX=2692176 GN=GTP27_05150 PE=4 SV=1
MM1 pKa = 7.22NAVADD6 pKa = 4.36AQDD9 pKa = 3.76VMPAPIIFTDD19 pKa = 3.7SAAEE23 pKa = 4.01KK24 pKa = 9.59VAQLIEE30 pKa = 4.38EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.58NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.65NPTASSTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L8L3U1|A0A6L8L3U1_9BURK S-formylglutathione hydrolase OS=Pseudoduganella sp. CY13W OX=2692176 GN=fghA PE=3 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.83TKK7 pKa = 10.72SSAKK11 pKa = 9.15KK12 pKa = 9.67RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.54SGMAFKK29 pKa = 10.69RR30 pKa = 11.84HH31 pKa = 5.84ILTKK35 pKa = 9.78KK36 pKa = 3.98TTKK39 pKa = 10.23NKK41 pKa = 9.21RR42 pKa = 11.84QLRR45 pKa = 11.84GTRR48 pKa = 11.84NINASDD54 pKa = 3.65VTSVLRR60 pKa = 11.84MMPSAA65 pKa = 4.45

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4524

0

4524

1580758

29

3391

349.4

38.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.654 ± 0.062

0.874 ± 0.012

5.159 ± 0.024

5.045 ± 0.036

3.428 ± 0.027

7.842 ± 0.039

2.215 ± 0.019

4.821 ± 0.028

3.585 ± 0.036

10.882 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.019

3.184 ± 0.028

4.69 ± 0.028

4.712 ± 0.04

6.114 ± 0.037

5.999 ± 0.036

5.225 ± 0.038

7.004 ± 0.034

1.317 ± 0.015

2.712 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski