Amino acid dipepetide frequency for Pseudoduganella sp. CY13W

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.696AlaAla: 18.696 ± 0.23
1.207AlaCys: 1.207 ± 0.03
6.417AlaAsp: 6.417 ± 0.07
6.453AlaGlu: 6.453 ± 0.079
3.55AlaPhe: 3.55 ± 0.05
10.404AlaGly: 10.404 ± 0.115
2.42AlaHis: 2.42 ± 0.045
5.928AlaIle: 5.928 ± 0.066
3.818AlaLys: 3.818 ± 0.07
14.591AlaLeu: 14.591 ± 0.228
3.511AlaMet: 3.511 ± 0.051
3.307AlaAsn: 3.307 ± 0.054
5.781AlaPro: 5.781 ± 0.083
6.52AlaGln: 6.52 ± 0.111
7.707AlaArg: 7.707 ± 0.092
7.856AlaSer: 7.856 ± 0.088
6.027AlaThr: 6.027 ± 0.064
8.037AlaVal: 8.037 ± 0.075
1.381AlaTrp: 1.381 ± 0.035
2.928AlaTyr: 2.928 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.152CysAla: 1.152 ± 0.028
0.125CysCys: 0.125 ± 0.01
0.504CysAsp: 0.504 ± 0.018
0.407CysGlu: 0.407 ± 0.018
0.27CysPhe: 0.27 ± 0.013
0.857CysGly: 0.857 ± 0.025
0.252CysHis: 0.252 ± 0.014
0.402CysIle: 0.402 ± 0.017
0.266CysLys: 0.266 ± 0.013
0.793CysLeu: 0.793 ± 0.027
0.206CysMet: 0.206 ± 0.012
0.245CysAsn: 0.245 ± 0.014
0.406CysPro: 0.406 ± 0.017
0.3CysGln: 0.3 ± 0.015
0.514CysArg: 0.514 ± 0.02
0.596CysSer: 0.596 ± 0.019
0.424CysThr: 0.424 ± 0.018
0.643CysVal: 0.643 ± 0.021
0.119CysTrp: 0.119 ± 0.01
0.256CysTyr: 0.256 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.468AspAla: 6.468 ± 0.081
0.427AspCys: 0.427 ± 0.019
2.664AspAsp: 2.664 ± 0.048
2.883AspGlu: 2.883 ± 0.05
2.047AspPhe: 2.047 ± 0.037
4.753AspGly: 4.753 ± 0.065
1.027AspHis: 1.027 ± 0.027
2.647AspIle: 2.647 ± 0.042
1.928AspLys: 1.928 ± 0.044
5.139AspLeu: 5.139 ± 0.053
1.327AspMet: 1.327 ± 0.03
1.527AspAsn: 1.527 ± 0.035
2.48AspPro: 2.48 ± 0.039
2.212AspGln: 2.212 ± 0.1
2.584AspArg: 2.584 ± 0.038
2.727AspSer: 2.727 ± 0.05
2.52AspThr: 2.52 ± 0.045
3.638AspVal: 3.638 ± 0.052
0.901AspTrp: 0.901 ± 0.027
1.695AspTyr: 1.695 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
5.809GluAla: 5.809 ± 0.068
0.338GluCys: 0.338 ± 0.016
1.963GluAsp: 1.963 ± 0.033
2.54GluGlu: 2.54 ± 0.051
1.864GluPhe: 1.864 ± 0.034
3.159GluGly: 3.159 ± 0.053
1.335GluHis: 1.335 ± 0.034
2.824GluIle: 2.824 ± 0.05
2.082GluLys: 2.082 ± 0.041
5.988GluLeu: 5.988 ± 0.092
1.313GluMet: 1.313 ± 0.028
1.572GluAsn: 1.572 ± 0.037
1.867GluPro: 1.867 ± 0.039
3.517GluGln: 3.517 ± 0.058
3.939GluArg: 3.939 ± 0.058
2.262GluSer: 2.262 ± 0.04
2.525GluThr: 2.525 ± 0.048
3.635GluVal: 3.635 ± 0.053
0.678GluTrp: 0.678 ± 0.019
1.244GluTyr: 1.244 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
3.867PheAla: 3.867 ± 0.053
0.388PheCys: 0.388 ± 0.017
2.355PheAsp: 2.355 ± 0.043
1.792PheGlu: 1.792 ± 0.035
1.293PhePhe: 1.293 ± 0.034
3.197PheGly: 3.197 ± 0.055
0.734PheHis: 0.734 ± 0.02
1.692PheIle: 1.692 ± 0.037
1.317PheLys: 1.317 ± 0.03
2.826PheLeu: 2.826 ± 0.051
0.836PheMet: 0.836 ± 0.024
1.41PheAsn: 1.41 ± 0.029
1.392PhePro: 1.392 ± 0.03
1.118PheGln: 1.118 ± 0.025
1.852PheArg: 1.852 ± 0.033
2.505PheSer: 2.505 ± 0.049
1.855PheThr: 1.855 ± 0.041
2.387PheVal: 2.387 ± 0.042
0.455PheTrp: 0.455 ± 0.017
1.013PheTyr: 1.013 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.539GlyAla: 8.539 ± 0.094
0.73GlyCys: 0.73 ± 0.025
3.672GlyAsp: 3.672 ± 0.054
3.918GlyGlu: 3.918 ± 0.054
2.95GlyPhe: 2.95 ± 0.046
6.257GlyGly: 6.257 ± 0.095
1.759GlyHis: 1.759 ± 0.037
4.008GlyIle: 4.008 ± 0.055
3.843GlyLys: 3.843 ± 0.062
8.12GlyLeu: 8.12 ± 0.087
2.337GlyMet: 2.337 ± 0.044
2.624GlyAsn: 2.624 ± 0.067
2.456GlyPro: 2.456 ± 0.041
3.534GlyGln: 3.534 ± 0.047
4.418GlyArg: 4.418 ± 0.061
5.058GlySer: 5.058 ± 0.069
4.214GlyThr: 4.214 ± 0.075
5.998GlyVal: 5.998 ± 0.074
1.32GlyTrp: 1.32 ± 0.03
2.666GlyTyr: 2.666 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.749HisAla: 2.749 ± 0.048
0.257HisCys: 0.257 ± 0.013
1.178HisAsp: 1.178 ± 0.028
1.066HisGlu: 1.066 ± 0.029
0.896HisPhe: 0.896 ± 0.029
2.004HisGly: 2.004 ± 0.041
0.634HisHis: 0.634 ± 0.021
1.033HisIle: 1.033 ± 0.025
0.641HisLys: 0.641 ± 0.02
2.253HisLeu: 2.253 ± 0.036
0.521HisMet: 0.521 ± 0.017
0.647HisAsn: 0.647 ± 0.021
1.265HisPro: 1.265 ± 0.029
0.916HisGln: 0.916 ± 0.024
1.192HisArg: 1.192 ± 0.027
1.248HisSer: 1.248 ± 0.026
1.13HisThr: 1.13 ± 0.03
1.353HisVal: 1.353 ± 0.03
0.391HisTrp: 0.391 ± 0.016
0.777HisTyr: 0.777 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
6.421IleAla: 6.421 ± 0.083
0.443IleCys: 0.443 ± 0.016
3.317IleAsp: 3.317 ± 0.05
3.082IleGlu: 3.082 ± 0.048
1.465IlePhe: 1.465 ± 0.034
4.24IleGly: 4.24 ± 0.058
0.936IleHis: 0.936 ± 0.026
2.174IleIle: 2.174 ± 0.041
1.894IleLys: 1.894 ± 0.042
3.886IleLeu: 3.886 ± 0.065
0.979IleMet: 0.979 ± 0.024
1.819IleAsn: 1.819 ± 0.038
2.083IlePro: 2.083 ± 0.044
1.416IleGln: 1.416 ± 0.026
2.74IleArg: 2.74 ± 0.045
3.307IleSer: 3.307 ± 0.053
2.746IleThr: 2.746 ± 0.047
3.519IleVal: 3.519 ± 0.05
0.56IleTrp: 0.56 ± 0.023
1.183IleTyr: 1.183 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.185LysAla: 4.185 ± 0.067
0.175LysCys: 0.175 ± 0.012
1.85LysAsp: 1.85 ± 0.046
1.741LysGlu: 1.741 ± 0.042
1.199LysPhe: 1.199 ± 0.027
2.351LysGly: 2.351 ± 0.05
0.737LysHis: 0.737 ± 0.021
1.893LysIle: 1.893 ± 0.039
1.626LysLys: 1.626 ± 0.042
4.203LysLeu: 4.203 ± 0.061
0.969LysMet: 0.969 ± 0.025
1.275LysAsn: 1.275 ± 0.031
1.973LysPro: 1.973 ± 0.04
1.693LysGln: 1.693 ± 0.039
2.117LysArg: 2.117 ± 0.04
1.922LysSer: 1.922 ± 0.039
1.981LysThr: 1.981 ± 0.036
2.727LysVal: 2.727 ± 0.05
0.383LysTrp: 0.383 ± 0.017
0.842LysTyr: 0.842 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.083LeuAla: 15.083 ± 0.136
1.068LeuCys: 1.068 ± 0.032
5.719LeuAsp: 5.719 ± 0.067
5.276LeuGlu: 5.276 ± 0.07
3.462LeuPhe: 3.462 ± 0.06
8.12LeuGly: 8.12 ± 0.098
2.45LeuHis: 2.45 ± 0.042
4.734LeuIle: 4.734 ± 0.072
3.929LeuLys: 3.929 ± 0.059
11.847LeuLeu: 11.847 ± 0.13
2.535LeuMet: 2.535 ± 0.041
3.48LeuAsn: 3.48 ± 0.069
5.879LeuPro: 5.879 ± 0.066
4.97LeuGln: 4.97 ± 0.069
7.231LeuArg: 7.231 ± 0.077
6.843LeuSer: 6.843 ± 0.126
5.731LeuThr: 5.731 ± 0.111
6.827LeuVal: 6.827 ± 0.085
1.198LeuTrp: 1.198 ± 0.033
2.437LeuTyr: 2.437 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.05MetAla: 3.05 ± 0.051
0.169MetCys: 0.169 ± 0.01
1.174MetAsp: 1.174 ± 0.029
1.16MetGlu: 1.16 ± 0.029
0.743MetPhe: 0.743 ± 0.021
1.724MetGly: 1.724 ± 0.037
0.576MetHis: 0.576 ± 0.021
1.026MetIle: 1.026 ± 0.029
1.058MetLys: 1.058 ± 0.024
3.035MetLeu: 3.035 ± 0.051
0.685MetMet: 0.685 ± 0.023
0.953MetAsn: 0.953 ± 0.025
1.451MetPro: 1.451 ± 0.036
1.316MetGln: 1.316 ± 0.026
1.592MetArg: 1.592 ± 0.03
1.647MetSer: 1.647 ± 0.029
1.531MetThr: 1.531 ± 0.034
1.754MetVal: 1.754 ± 0.031
0.232MetTrp: 0.232 ± 0.011
0.483MetTyr: 0.483 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.619AsnAla: 3.619 ± 0.058
0.283AsnCys: 0.283 ± 0.013
1.6AsnAsp: 1.6 ± 0.033
1.387AsnGlu: 1.387 ± 0.031
1.225AsnPhe: 1.225 ± 0.029
2.911AsnGly: 2.911 ± 0.062
0.64AsnHis: 0.64 ± 0.021
1.641AsnIle: 1.641 ± 0.041
1.084AsnLys: 1.084 ± 0.028
3.276AsnLeu: 3.276 ± 0.052
0.745AsnMet: 0.745 ± 0.025
1.172AsnAsn: 1.172 ± 0.034
1.793AsnPro: 1.793 ± 0.037
1.327AsnGln: 1.327 ± 0.038
1.743AsnArg: 1.743 ± 0.034
1.819AsnSer: 1.819 ± 0.04
1.839AsnThr: 1.839 ± 0.044
2.231AsnVal: 2.231 ± 0.045
0.493AsnTrp: 0.493 ± 0.019
1.008AsnTyr: 1.008 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
7.135ProAla: 7.135 ± 0.095
0.311ProCys: 0.311 ± 0.015
2.847ProAsp: 2.847 ± 0.046
2.935ProGlu: 2.935 ± 0.046
1.611ProPhe: 1.611 ± 0.031
3.45ProGly: 3.45 ± 0.054
0.991ProHis: 0.991 ± 0.027
1.794ProIle: 1.794 ± 0.035
1.409ProLys: 1.409 ± 0.033
5.012ProLeu: 5.012 ± 0.06
1.127ProMet: 1.127 ± 0.027
1.331ProAsn: 1.331 ± 0.028
2.11ProPro: 2.11 ± 0.053
2.3ProGln: 2.3 ± 0.039
2.339ProArg: 2.339 ± 0.046
2.341ProSer: 2.341 ± 0.041
2.105ProThr: 2.105 ± 0.039
3.922ProVal: 3.922 ± 0.052
0.593ProTrp: 0.593 ± 0.02
1.235ProTyr: 1.235 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
6.029GlnAla: 6.029 ± 0.086
0.325GlnCys: 0.325 ± 0.016
1.752GlnAsp: 1.752 ± 0.036
1.904GlnGlu: 1.904 ± 0.039
1.622GlnPhe: 1.622 ± 0.038
3.181GlnGly: 3.181 ± 0.046
1.295GlnHis: 1.295 ± 0.032
2.495GlnIle: 2.495 ± 0.1
1.358GlnLys: 1.358 ± 0.029
5.724GlnLeu: 5.724 ± 0.122
1.163GlnMet: 1.163 ± 0.03
1.248GlnAsn: 1.248 ± 0.029
2.452GlnPro: 2.452 ± 0.047
3.228GlnGln: 3.228 ± 0.063
3.749GlnArg: 3.749 ± 0.053
2.367GlnSer: 2.367 ± 0.041
2.25GlnThr: 2.25 ± 0.042
3.236GlnVal: 3.236 ± 0.076
0.634GlnTrp: 0.634 ± 0.021
1.11GlnTyr: 1.11 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
6.765ArgAla: 6.765 ± 0.07
0.542ArgCys: 0.542 ± 0.018
3.187ArgAsp: 3.187 ± 0.045
3.359ArgGlu: 3.359 ± 0.05
2.266ArgPhe: 2.266 ± 0.038
3.811ArgGly: 3.811 ± 0.054
1.657ArgHis: 1.657 ± 0.036
3.364ArgIle: 3.364 ± 0.048
2.262ArgLys: 2.262 ± 0.038
6.842ArgLeu: 6.842 ± 0.093
1.728ArgMet: 1.728 ± 0.03
2.073ArgAsn: 2.073 ± 0.036
2.544ArgPro: 2.544 ± 0.043
3.181ArgGln: 3.181 ± 0.048
3.989ArgArg: 3.989 ± 0.063
3.596ArgSer: 3.596 ± 0.06
2.902ArgThr: 2.902 ± 0.04
3.958ArgVal: 3.958 ± 0.047
0.987ArgTrp: 0.987 ± 0.026
2.132ArgTyr: 2.132 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.56SerAla: 7.56 ± 0.085
0.481SerCys: 0.481 ± 0.02
3.123SerAsp: 3.123 ± 0.06
2.729SerGlu: 2.729 ± 0.044
2.239SerPhe: 2.239 ± 0.037
5.602SerGly: 5.602 ± 0.073
1.341SerHis: 1.341 ± 0.03
2.89SerIle: 2.89 ± 0.049
2.173SerLys: 2.173 ± 0.049
5.941SerLeu: 5.941 ± 0.073
1.476SerMet: 1.476 ± 0.028
1.987SerAsn: 1.987 ± 0.044
2.461SerPro: 2.461 ± 0.046
2.195SerGln: 2.195 ± 0.057
3.3SerArg: 3.3 ± 0.046
4.054SerSer: 4.054 ± 0.076
3.469SerThr: 3.469 ± 0.093
4.319SerVal: 4.319 ± 0.058
0.796SerTrp: 0.796 ± 0.025
1.854SerTyr: 1.854 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.377ThrAla: 6.377 ± 0.111
0.378ThrCys: 0.378 ± 0.017
2.51ThrAsp: 2.51 ± 0.067
2.4ThrGlu: 2.4 ± 0.038
1.621ThrPhe: 1.621 ± 0.033
4.477ThrGly: 4.477 ± 0.064
1.001ThrHis: 1.001 ± 0.023
2.572ThrIle: 2.572 ± 0.042
1.329ThrLys: 1.329 ± 0.03
6.704ThrLeu: 6.704 ± 0.075
1.198ThrMet: 1.198 ± 0.026
1.461ThrAsn: 1.461 ± 0.043
3.329ThrPro: 3.329 ± 0.047
1.912ThrGln: 1.912 ± 0.044
2.984ThrArg: 2.984 ± 0.049
3.107ThrSer: 3.107 ± 0.071
2.911ThrThr: 2.911 ± 0.09
4.105ThrVal: 4.105 ± 0.064
0.523ThrTrp: 0.523 ± 0.022
1.354ThrTyr: 1.354 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.911ValAla: 8.911 ± 0.091
0.658ValCys: 0.658 ± 0.02
3.617ValAsp: 3.617 ± 0.055
3.684ValGlu: 3.684 ± 0.049
2.331ValPhe: 2.331 ± 0.038
4.838ValGly: 4.838 ± 0.065
1.393ValHis: 1.393 ± 0.028
3.436ValIle: 3.436 ± 0.057
2.514ValLys: 2.514 ± 0.05
7.879ValLeu: 7.879 ± 0.096
1.783ValMet: 1.783 ± 0.032
2.306ValAsn: 2.306 ± 0.045
3.312ValPro: 3.312 ± 0.043
3.134ValGln: 3.134 ± 0.046
4.259ValArg: 4.259 ± 0.057
4.248ValSer: 4.248 ± 0.06
3.915ValThr: 3.915 ± 0.069
5.235ValVal: 5.235 ± 0.071
0.874ValTrp: 0.874 ± 0.034
1.713ValTyr: 1.713 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.055TrpAla: 1.055 ± 0.029
0.142TrpCys: 0.142 ± 0.009
0.616TrpAsp: 0.616 ± 0.023
0.54TrpGlu: 0.54 ± 0.019
0.499TrpPhe: 0.499 ± 0.017
0.794TrpGly: 0.794 ± 0.028
0.35TrpHis: 0.35 ± 0.014
0.621TrpIle: 0.621 ± 0.022
0.457TrpLys: 0.457 ± 0.017
1.873TrpLeu: 1.873 ± 0.045
0.375TrpMet: 0.375 ± 0.018
0.496TrpAsn: 0.496 ± 0.017
0.573TrpPro: 0.573 ± 0.021
0.933TrpGln: 0.933 ± 0.024
1.064TrpArg: 1.064 ± 0.029
0.786TrpSer: 0.786 ± 0.025
0.626TrpThr: 0.626 ± 0.022
0.777TrpVal: 0.777 ± 0.023
0.234TrpTrp: 0.234 ± 0.014
0.356TrpTyr: 0.356 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.068TyrAla: 3.068 ± 0.048
0.293TyrCys: 0.293 ± 0.014
1.529TyrAsp: 1.529 ± 0.034
1.2TyrGlu: 1.2 ± 0.031
1.129TyrPhe: 1.129 ± 0.027
2.29TyrGly: 2.29 ± 0.044
0.626TyrHis: 0.626 ± 0.021
1.037TyrIle: 1.037 ± 0.027
0.857TyrLys: 0.857 ± 0.026
2.887TyrLeu: 2.887 ± 0.045
0.545TyrMet: 0.545 ± 0.017
0.903TyrAsn: 0.903 ± 0.03
1.289TyrPro: 1.289 ± 0.028
1.397TyrGln: 1.397 ± 0.034
1.83TyrArg: 1.83 ± 0.039
1.702TyrSer: 1.702 ± 0.04
1.552TyrThr: 1.552 ± 0.037
1.738TyrVal: 1.738 ± 0.034
0.417TyrTrp: 0.417 ± 0.018
0.838TyrTyr: 0.838 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4524 proteins (1580758 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski