Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Spbetavirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O64053|O64053_BPSPB YomQ protein OS=Bacillus phage SPbeta OX=66797 GN=yomQ PE=4 SV=1
MM1 pKa = 7.52SNFWVIALNKK11 pKa = 9.7NWATLDD17 pKa = 3.63QVKK20 pKa = 9.61EE21 pKa = 3.73AYY23 pKa = 10.47YY24 pKa = 10.68YY25 pKa = 11.21DD26 pKa = 4.99DD27 pKa = 3.56VTKK30 pKa = 10.94EE31 pKa = 3.94EE32 pKa = 4.32LKK34 pKa = 11.08EE35 pKa = 4.12GVDD38 pKa = 3.73NNLITPEE45 pKa = 3.98QYY47 pKa = 10.35QEE49 pKa = 4.17IVGEE53 pKa = 4.44AYY55 pKa = 9.72TSVTLSTEE63 pKa = 3.68

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O64082|O64082_BPSPB Uncharacterized protein yonV OS=Bacillus phage SPbeta OX=66797 GN=yonV PE=4 SV=1
MM1 pKa = 7.43LEE3 pKa = 3.73KK4 pKa = 10.15MGIVVAFLISLTVLTINSLTIVEE27 pKa = 4.5KK28 pKa = 10.96VRR30 pKa = 11.84NLKK33 pKa = 10.62NGTSKK38 pKa = 10.51KK39 pKa = 9.76KK40 pKa = 9.88KK41 pKa = 9.54RR42 pKa = 11.84IRR44 pKa = 11.84KK45 pKa = 8.26RR46 pKa = 11.84LRR48 pKa = 11.84PKK50 pKa = 10.01RR51 pKa = 11.84QRR53 pKa = 11.84QRR55 pKa = 11.84IRR57 pKa = 11.84RR58 pKa = 3.78

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

185

0

185

38336

21

2285

207.2

23.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.008 ± 0.275

0.976 ± 0.094

6.211 ± 0.133

8.058 ± 0.235

4.333 ± 0.182

5.402 ± 0.155

1.839 ± 0.134

7.612 ± 0.188

9.625 ± 0.216

8.78 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.09

6.195 ± 0.153

2.64 ± 0.122

3.574 ± 0.259

3.673 ± 0.15

6.751 ± 0.224

5.368 ± 0.164

5.971 ± 0.161

1.054 ± 0.084

4.575 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski