Amino acid dipepetide frequency for Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.809AlaAla: 3.809 ± 0.41
0.522AlaCys: 0.522 ± 0.143
3.209AlaAsp: 3.209 ± 0.284
3.0AlaGlu: 3.0 ± 0.278
2.061AlaPhe: 2.061 ± 0.248
2.713AlaGly: 2.713 ± 0.319
0.783AlaHis: 0.783 ± 0.127
4.304AlaIle: 4.304 ± 0.308
5.322AlaLys: 5.322 ± 0.445
4.409AlaLeu: 4.409 ± 0.391
1.07AlaMet: 1.07 ± 0.182
2.217AlaAsn: 2.217 ± 0.26
1.226AlaPro: 1.226 ± 0.165
1.774AlaGln: 1.774 ± 0.343
1.669AlaArg: 1.669 ± 0.231
3.104AlaSer: 3.104 ± 0.405
2.843AlaThr: 2.843 ± 0.229
3.156AlaVal: 3.156 ± 0.268
0.496AlaTrp: 0.496 ± 0.114
2.4AlaTyr: 2.4 ± 0.267
0.0AlaXaa: 0.0 ± 0.0
Cys
0.287CysAla: 0.287 ± 0.103
0.183CysCys: 0.183 ± 0.071
0.417CysAsp: 0.417 ± 0.116
0.73CysGlu: 0.73 ± 0.164
0.756CysPhe: 0.756 ± 0.166
0.652CysGly: 0.652 ± 0.135
0.287CysHis: 0.287 ± 0.087
0.678CysIle: 0.678 ± 0.16
0.443CysLys: 0.443 ± 0.12
0.965CysLeu: 0.965 ± 0.158
0.235CysMet: 0.235 ± 0.079
0.548CysAsn: 0.548 ± 0.111
0.313CysPro: 0.313 ± 0.106
0.287CysGln: 0.287 ± 0.074
0.522CysArg: 0.522 ± 0.121
0.704CysSer: 0.704 ± 0.137
0.47CysThr: 0.47 ± 0.137
0.574CysVal: 0.574 ± 0.146
0.287CysTrp: 0.287 ± 0.082
0.417CysTyr: 0.417 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
2.609AspAla: 2.609 ± 0.281
0.704AspCys: 0.704 ± 0.148
3.443AspAsp: 3.443 ± 0.346
5.008AspGlu: 5.008 ± 0.353
3.287AspPhe: 3.287 ± 0.235
4.069AspGly: 4.069 ± 0.337
1.07AspHis: 1.07 ± 0.196
5.556AspIle: 5.556 ± 0.374
5.4AspLys: 5.4 ± 0.441
5.374AspLeu: 5.374 ± 0.349
1.513AspMet: 1.513 ± 0.18
3.391AspAsn: 3.391 ± 0.291
2.035AspPro: 2.035 ± 0.22
2.009AspGln: 2.009 ± 0.203
2.009AspArg: 2.009 ± 0.254
4.069AspSer: 4.069 ± 0.298
2.609AspThr: 2.609 ± 0.302
3.835AspVal: 3.835 ± 0.33
0.678AspTrp: 0.678 ± 0.131
3.443AspTyr: 3.443 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
4.043GluAla: 4.043 ± 0.382
0.756GluCys: 0.756 ± 0.158
4.435GluAsp: 4.435 ± 0.31
7.069GluGlu: 7.069 ± 0.529
3.235GluPhe: 3.235 ± 0.25
3.939GluGly: 3.939 ± 0.36
1.539GluHis: 1.539 ± 0.223
6.704GluIle: 6.704 ± 0.431
7.487GluLys: 7.487 ± 0.474
8.113GluLeu: 8.113 ± 0.472
2.4GluMet: 2.4 ± 0.252
4.695GluAsn: 4.695 ± 0.429
1.539GluPro: 1.539 ± 0.234
3.182GluGln: 3.182 ± 0.331
3.469GluArg: 3.469 ± 0.385
4.148GluSer: 4.148 ± 0.386
3.626GluThr: 3.626 ± 0.301
5.165GluVal: 5.165 ± 0.45
1.252GluTrp: 1.252 ± 0.222
3.756GluTyr: 3.756 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
1.983PheAla: 1.983 ± 0.183
0.365PheCys: 0.365 ± 0.103
3.235PheAsp: 3.235 ± 0.263
3.965PheGlu: 3.965 ± 0.354
2.243PhePhe: 2.243 ± 0.296
2.217PheGly: 2.217 ± 0.233
1.122PheHis: 1.122 ± 0.152
3.078PheIle: 3.078 ± 0.267
4.095PheLys: 4.095 ± 0.335
3.548PheLeu: 3.548 ± 0.367
1.096PheMet: 1.096 ± 0.198
3.052PheAsn: 3.052 ± 0.266
1.252PhePro: 1.252 ± 0.18
0.913PheGln: 0.913 ± 0.151
1.487PheArg: 1.487 ± 0.15
2.948PheSer: 2.948 ± 0.274
1.93PheThr: 1.93 ± 0.216
2.504PheVal: 2.504 ± 0.306
0.417PheTrp: 0.417 ± 0.105
1.878PheTyr: 1.878 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
2.243GlyAla: 2.243 ± 0.228
0.6GlyCys: 0.6 ± 0.12
3.0GlyAsp: 3.0 ± 0.288
4.069GlyGlu: 4.069 ± 0.341
2.504GlyPhe: 2.504 ± 0.204
3.052GlyGly: 3.052 ± 0.366
0.861GlyHis: 0.861 ± 0.152
4.278GlyIle: 4.278 ± 0.409
4.878GlyLys: 4.878 ± 0.368
4.722GlyLeu: 4.722 ± 0.31
1.539GlyMet: 1.539 ± 0.241
3.391GlyAsn: 3.391 ± 0.31
0.313GlyPro: 0.313 ± 0.106
2.087GlyGln: 2.087 ± 0.238
1.8GlyArg: 1.8 ± 0.207
3.626GlySer: 3.626 ± 0.308
3.626GlyThr: 3.626 ± 0.335
3.73GlyVal: 3.73 ± 0.298
0.548GlyTrp: 0.548 ± 0.112
3.156GlyTyr: 3.156 ± 0.33
0.0GlyXaa: 0.0 ± 0.0
His
0.574HisAla: 0.574 ± 0.116
0.287HisCys: 0.287 ± 0.088
0.809HisAsp: 0.809 ± 0.142
1.356HisGlu: 1.356 ± 0.231
0.809HisPhe: 0.809 ± 0.151
0.991HisGly: 0.991 ± 0.151
0.391HisHis: 0.391 ± 0.093
1.33HisIle: 1.33 ± 0.175
1.852HisLys: 1.852 ± 0.297
1.93HisLeu: 1.93 ± 0.196
0.339HisMet: 0.339 ± 0.096
1.591HisAsn: 1.591 ± 0.24
0.73HisPro: 0.73 ± 0.142
0.496HisGln: 0.496 ± 0.124
0.652HisArg: 0.652 ± 0.135
1.33HisSer: 1.33 ± 0.203
0.965HisThr: 0.965 ± 0.171
1.07HisVal: 1.07 ± 0.153
0.183HisTrp: 0.183 ± 0.076
0.704HisTyr: 0.704 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.122IleAla: 4.122 ± 0.347
0.652IleCys: 0.652 ± 0.159
6.078IleAsp: 6.078 ± 0.397
6.782IleGlu: 6.782 ± 0.45
2.556IlePhe: 2.556 ± 0.244
4.043IleGly: 4.043 ± 0.364
1.852IleHis: 1.852 ± 0.279
4.695IleIle: 4.695 ± 0.455
7.513IleLys: 7.513 ± 0.425
5.635IleLeu: 5.635 ± 0.544
1.461IleMet: 1.461 ± 0.207
5.165IleAsn: 5.165 ± 0.352
2.791IlePro: 2.791 ± 0.221
2.791IleGln: 2.791 ± 0.299
2.739IleArg: 2.739 ± 0.278
4.722IleSer: 4.722 ± 0.341
4.774IleThr: 4.774 ± 0.35
4.304IleVal: 4.304 ± 0.365
0.835IleTrp: 0.835 ± 0.178
2.609IleTyr: 2.609 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
5.008LysAla: 5.008 ± 0.469
0.965LysCys: 0.965 ± 0.196
6.261LysAsp: 6.261 ± 0.379
8.947LysGlu: 8.947 ± 0.613
3.391LysPhe: 3.391 ± 0.326
5.556LysGly: 5.556 ± 0.402
1.826LysHis: 1.826 ± 0.249
6.182LysIle: 6.182 ± 0.359
9.73LysLys: 9.73 ± 0.576
8.191LysLeu: 8.191 ± 0.5
2.087LysMet: 2.087 ± 0.254
5.504LysAsn: 5.504 ± 0.431
2.061LysPro: 2.061 ± 0.279
3.835LysGln: 3.835 ± 0.365
4.409LysArg: 4.409 ± 0.371
5.713LysSer: 5.713 ± 0.483
5.217LysThr: 5.217 ± 0.463
6.078LysVal: 6.078 ± 0.476
0.756LysTrp: 0.756 ± 0.161
4.539LysTyr: 4.539 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
4.356LeuAla: 4.356 ± 0.34
0.756LeuCys: 0.756 ± 0.128
5.165LeuAsp: 5.165 ± 0.274
5.974LeuGlu: 5.974 ± 0.509
4.043LeuPhe: 4.043 ± 0.38
4.226LeuGly: 4.226 ± 0.309
1.617LeuHis: 1.617 ± 0.21
6.991LeuIle: 6.991 ± 0.583
8.295LeuLys: 8.295 ± 0.484
8.113LeuLeu: 8.113 ± 0.582
2.113LeuMet: 2.113 ± 0.208
6.652LeuAsn: 6.652 ± 0.426
2.348LeuPro: 2.348 ± 0.244
3.156LeuGln: 3.156 ± 0.474
3.235LeuArg: 3.235 ± 0.319
6.652LeuSer: 6.652 ± 0.44
5.139LeuThr: 5.139 ± 0.315
4.382LeuVal: 4.382 ± 0.343
0.756LeuTrp: 0.756 ± 0.141
3.835LeuTyr: 3.835 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
1.461MetAla: 1.461 ± 0.171
0.157MetCys: 0.157 ± 0.064
1.513MetAsp: 1.513 ± 0.195
1.93MetGlu: 1.93 ± 0.229
1.07MetPhe: 1.07 ± 0.17
1.278MetGly: 1.278 ± 0.162
0.235MetHis: 0.235 ± 0.081
1.617MetIle: 1.617 ± 0.23
2.791MetLys: 2.791 ± 0.293
1.591MetLeu: 1.591 ± 0.204
0.626MetMet: 0.626 ± 0.13
1.539MetAsn: 1.539 ± 0.184
0.756MetPro: 0.756 ± 0.145
0.965MetGln: 0.965 ± 0.21
0.704MetArg: 0.704 ± 0.112
2.061MetSer: 2.061 ± 0.268
1.33MetThr: 1.33 ± 0.174
1.2MetVal: 1.2 ± 0.158
0.261MetTrp: 0.261 ± 0.093
0.47MetTyr: 0.47 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
3.209AsnAla: 3.209 ± 0.373
0.652AsnCys: 0.652 ± 0.144
4.095AsnAsp: 4.095 ± 0.315
4.93AsnGlu: 4.93 ± 0.383
2.374AsnPhe: 2.374 ± 0.236
3.782AsnGly: 3.782 ± 0.399
0.965AsnHis: 0.965 ± 0.138
5.008AsnIle: 5.008 ± 0.378
6.861AsnLys: 6.861 ± 0.422
4.617AsnLeu: 4.617 ± 0.347
1.409AsnMet: 1.409 ± 0.194
3.809AsnAsn: 3.809 ± 0.295
1.93AsnPro: 1.93 ± 0.224
1.983AsnGln: 1.983 ± 0.195
2.269AsnArg: 2.269 ± 0.24
3.965AsnSer: 3.965 ± 0.347
3.261AsnThr: 3.261 ± 0.373
3.469AsnVal: 3.469 ± 0.332
0.678AsnTrp: 0.678 ± 0.121
2.687AsnTyr: 2.687 ± 0.276
0.0AsnXaa: 0.0 ± 0.0
Pro
1.278ProAla: 1.278 ± 0.192
0.235ProCys: 0.235 ± 0.078
1.878ProAsp: 1.878 ± 0.216
2.374ProGlu: 2.374 ± 0.264
1.148ProPhe: 1.148 ± 0.197
0.913ProGly: 0.913 ± 0.131
0.783ProHis: 0.783 ± 0.163
1.956ProIle: 1.956 ± 0.25
2.426ProLys: 2.426 ± 0.238
2.609ProLeu: 2.609 ± 0.284
0.522ProMet: 0.522 ± 0.113
1.591ProAsn: 1.591 ± 0.206
0.496ProPro: 0.496 ± 0.114
0.73ProGln: 0.73 ± 0.132
0.913ProArg: 0.913 ± 0.176
2.191ProSer: 2.191 ± 0.219
1.252ProThr: 1.252 ± 0.2
1.278ProVal: 1.278 ± 0.212
0.287ProTrp: 0.287 ± 0.079
1.539ProTyr: 1.539 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
2.113GlnAla: 2.113 ± 0.336
0.261GlnCys: 0.261 ± 0.097
1.617GlnAsp: 1.617 ± 0.208
2.817GlnGlu: 2.817 ± 0.355
1.591GlnPhe: 1.591 ± 0.199
1.617GlnGly: 1.617 ± 0.211
0.417GlnHis: 0.417 ± 0.11
3.026GlnIle: 3.026 ± 0.317
3.574GlnLys: 3.574 ± 0.423
3.6GlnLeu: 3.6 ± 0.326
0.939GlnMet: 0.939 ± 0.137
2.4GlnAsn: 2.4 ± 0.287
0.73GlnPro: 0.73 ± 0.123
1.904GlnGln: 1.904 ± 0.417
1.252GlnArg: 1.252 ± 0.185
2.087GlnSer: 2.087 ± 0.29
1.513GlnThr: 1.513 ± 0.2
2.556GlnVal: 2.556 ± 0.208
0.313GlnTrp: 0.313 ± 0.085
1.409GlnTyr: 1.409 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
1.409ArgAla: 1.409 ± 0.208
0.287ArgCys: 0.287 ± 0.098
1.643ArgAsp: 1.643 ± 0.206
3.182ArgGlu: 3.182 ± 0.33
1.904ArgPhe: 1.904 ± 0.225
2.061ArgGly: 2.061 ± 0.244
0.809ArgHis: 0.809 ± 0.13
2.896ArgIle: 2.896 ± 0.254
3.391ArgLys: 3.391 ± 0.298
3.235ArgLeu: 3.235 ± 0.323
1.148ArgMet: 1.148 ± 0.161
2.478ArgAsn: 2.478 ± 0.268
0.809ArgPro: 0.809 ± 0.157
1.409ArgGln: 1.409 ± 0.182
1.487ArgArg: 1.487 ± 0.215
2.426ArgSer: 2.426 ± 0.26
1.878ArgThr: 1.878 ± 0.215
2.035ArgVal: 2.035 ± 0.217
0.443ArgTrp: 0.443 ± 0.112
1.8ArgTyr: 1.8 ± 0.222
0.0ArgXaa: 0.0 ± 0.0
Ser
3.052SerAla: 3.052 ± 0.323
0.783SerCys: 0.783 ± 0.157
4.278SerAsp: 4.278 ± 0.415
5.165SerGlu: 5.165 ± 0.377
3.391SerPhe: 3.391 ± 0.292
3.678SerGly: 3.678 ± 0.361
0.965SerHis: 0.965 ± 0.124
5.322SerIle: 5.322 ± 0.472
6.443SerLys: 6.443 ± 0.444
6.521SerLeu: 6.521 ± 0.452
1.539SerMet: 1.539 ± 0.232
4.435SerAsn: 4.435 ± 0.31
2.217SerPro: 2.217 ± 0.285
1.983SerGln: 1.983 ± 0.204
1.774SerArg: 1.774 ± 0.198
5.243SerSer: 5.243 ± 0.688
3.626SerThr: 3.626 ± 0.434
3.913SerVal: 3.913 ± 0.303
0.704SerTrp: 0.704 ± 0.136
2.478SerTyr: 2.478 ± 0.265
0.0SerXaa: 0.0 ± 0.0
Thr
2.896ThrAla: 2.896 ± 0.41
0.417ThrCys: 0.417 ± 0.104
2.922ThrAsp: 2.922 ± 0.255
4.278ThrGlu: 4.278 ± 0.316
1.904ThrPhe: 1.904 ± 0.257
3.522ThrGly: 3.522 ± 0.287
0.756ThrHis: 0.756 ± 0.159
3.913ThrIle: 3.913 ± 0.318
4.539ThrLys: 4.539 ± 0.295
4.643ThrLeu: 4.643 ± 0.35
1.017ThrMet: 1.017 ± 0.155
2.739ThrAsn: 2.739 ± 0.288
1.617ThrPro: 1.617 ± 0.186
2.139ThrGln: 2.139 ± 0.26
1.956ThrArg: 1.956 ± 0.189
3.939ThrSer: 3.939 ± 0.399
3.026ThrThr: 3.026 ± 0.277
4.043ThrVal: 4.043 ± 0.294
0.496ThrTrp: 0.496 ± 0.106
2.922ThrTyr: 2.922 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
3.313ValAla: 3.313 ± 0.284
0.678ValCys: 0.678 ± 0.148
4.017ValAsp: 4.017 ± 0.324
4.33ValGlu: 4.33 ± 0.357
2.4ValPhe: 2.4 ± 0.277
3.026ValGly: 3.026 ± 0.304
1.148ValHis: 1.148 ± 0.197
4.148ValIle: 4.148 ± 0.346
5.974ValLys: 5.974 ± 0.403
4.8ValLeu: 4.8 ± 0.451
1.461ValMet: 1.461 ± 0.207
3.756ValAsn: 3.756 ± 0.345
1.748ValPro: 1.748 ± 0.215
2.113ValGln: 2.113 ± 0.215
2.113ValArg: 2.113 ± 0.228
4.2ValSer: 4.2 ± 0.426
3.574ValThr: 3.574 ± 0.316
3.182ValVal: 3.182 ± 0.293
0.522ValTrp: 0.522 ± 0.107
3.209ValTyr: 3.209 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
0.496TrpAla: 0.496 ± 0.107
0.078TrpCys: 0.078 ± 0.045
0.835TrpAsp: 0.835 ± 0.145
1.07TrpGlu: 1.07 ± 0.203
0.47TrpPhe: 0.47 ± 0.116
0.574TrpGly: 0.574 ± 0.118
0.104TrpHis: 0.104 ± 0.052
0.939TrpIle: 0.939 ± 0.173
0.783TrpLys: 0.783 ± 0.121
0.991TrpLeu: 0.991 ± 0.162
0.183TrpMet: 0.183 ± 0.071
0.496TrpAsn: 0.496 ± 0.129
0.078TrpPro: 0.078 ± 0.045
0.261TrpGln: 0.261 ± 0.085
0.496TrpArg: 0.496 ± 0.131
0.652TrpSer: 0.652 ± 0.122
0.47TrpThr: 0.47 ± 0.1
0.835TrpVal: 0.835 ± 0.132
0.157TrpTrp: 0.157 ± 0.066
0.574TrpTyr: 0.574 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.826TyrAla: 1.826 ± 0.224
0.417TyrCys: 0.417 ± 0.114
3.261TyrAsp: 3.261 ± 0.274
3.6TyrGlu: 3.6 ± 0.341
2.191TyrPhe: 2.191 ± 0.266
2.113TyrGly: 2.113 ± 0.216
0.861TyrHis: 0.861 ± 0.129
3.496TyrIle: 3.496 ± 0.322
4.461TyrLys: 4.461 ± 0.331
4.2TyrLeu: 4.2 ± 0.326
0.835TyrMet: 0.835 ± 0.133
2.504TyrAsn: 2.504 ± 0.259
1.409TyrPro: 1.409 ± 0.174
1.722TyrGln: 1.722 ± 0.191
1.774TyrArg: 1.774 ± 0.208
3.73TyrSer: 3.73 ± 0.31
2.556TyrThr: 2.556 ± 0.21
2.4TyrVal: 2.4 ± 0.248
0.47TyrTrp: 0.47 ± 0.1
1.93TyrTyr: 1.93 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 185 proteins (38336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski