Mesosutterella multiformis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2198 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A388SC22|A0A388SC22_9BURK Uncharacterized protein OS=Mesosutterella multiformis OX=2259133 GN=MESMUL_12310 PE=4 SV=1
MM1 pKa = 7.97 SGMMTNDD8 pKa = 3.62 PLSVLKK14 pKa = 9.63 TLEE17 pKa = 3.65 ASRR20 pKa = 11.84 IKK22 pKa = 10.68 PEE24 pKa = 3.46 TRR26 pKa = 11.84 MQCKK30 pKa = 9.56 VCGFVYY36 pKa = 10.46 DD37 pKa = 3.88 PAEE40 pKa = 4.45 GCEE43 pKa = 4.15 EE44 pKa = 4.09 WQVPPGTPFAEE55 pKa = 4.22 IPDD58 pKa = 4.43 NYY60 pKa = 10.69 DD61 pKa = 4.03 CPACGCPHH69 pKa = 6.98 HH70 pKa = 6.85 SFIVLDD76 pKa = 4.41 DD77 pKa = 4.38 DD78 pKa = 4.99 EE79 pKa = 4.76 EE80 pKa = 4.46 VV81 pKa = 3.18
Molecular weight: 8.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.977
IPC2_protein 4.202
IPC_protein 4.101
Toseland 3.923
ProMoST 4.24
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.973
Rodwell 3.935
Grimsley 3.834
Solomon 4.05
Lehninger 4.012
Nozaki 4.19
DTASelect 4.342
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.215
Patrickios 0.604
IPC_peptide 4.05
IPC2_peptide 4.202
IPC2.peptide.svr19 4.098
Protein with the highest isoelectric point:
>tr|A0A388SCB1|A0A388SCB1_9BURK Uncharacterized protein OS=Mesosutterella multiformis OX=2259133 GN=MESMUL_04650 PE=4 SV=1
MM1 pKa = 6.85 ATKK4 pKa = 9.51 RR5 pKa = 11.84 TYY7 pKa = 10.29 QPSKK11 pKa = 9.15 IKK13 pKa = 10.36 RR14 pKa = 11.84 ARR16 pKa = 11.84 THH18 pKa = 5.93 GFLVRR23 pKa = 11.84 SRR25 pKa = 11.84 TRR27 pKa = 11.84 GGRR30 pKa = 11.84 KK31 pKa = 8.89 VLAARR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 10.23 GRR41 pKa = 11.84 HH42 pKa = 4.25 TLALL46 pKa = 3.89
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2198
0
2198
705911
39
1890
321.2
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.653 ± 0.075
1.111 ± 0.021
5.364 ± 0.042
6.355 ± 0.053
4.012 ± 0.037
7.95 ± 0.048
1.896 ± 0.022
5.702 ± 0.04
5.207 ± 0.046
9.503 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.023
3.142 ± 0.035
4.92 ± 0.036
2.905 ± 0.03
6.015 ± 0.046
6.241 ± 0.05
5.588 ± 0.048
7.145 ± 0.047
1.257 ± 0.019
2.565 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here