Sphingomonas sp. IC081

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4786 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514WD45|A0A514WD45_9SPHN Uncharacterized protein OS=Sphingomonas sp. IC081 OX=304378 GN=DM450_01110 PE=4 SV=1
MM1 pKa = 6.58TTVSSTSSSSSDD13 pKa = 3.21STSAATTSLLSDD25 pKa = 3.53YY26 pKa = 11.59NLFLKK31 pKa = 10.92LLTTQMTNQDD41 pKa = 4.33PLNPTDD47 pKa = 3.41TSEE50 pKa = 3.99YY51 pKa = 8.33TQQLVQYY58 pKa = 9.02SQVEE62 pKa = 3.91QQIQQNGTLTDD73 pKa = 3.33ILSQLTSSQMAQYY86 pKa = 11.06SSFIGKK92 pKa = 8.85DD93 pKa = 3.05ARR95 pKa = 11.84FDD97 pKa = 3.66SAVAGLGDD105 pKa = 5.71DD106 pKa = 3.99PAHH109 pKa = 5.14WTYY112 pKa = 11.27YY113 pKa = 10.52VNGTPASVTATISDD127 pKa = 3.81ANGKK131 pKa = 8.57VVQTVTLDD139 pKa = 3.43PEE141 pKa = 4.39AQGEE145 pKa = 4.37YY146 pKa = 9.94QWDD149 pKa = 3.8GTQADD154 pKa = 4.29GTTAASGAYY163 pKa = 8.0TLAVTATDD171 pKa = 3.54AAGNTLDD178 pKa = 3.5TTINSVARR186 pKa = 11.84VSDD189 pKa = 3.75VVTDD193 pKa = 4.26GSTIMLGVNGIRR205 pKa = 11.84MSVDD209 pKa = 2.83GLVALAATSSEE220 pKa = 4.0II221 pKa = 4.21

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514WMH8|A0A514WMH8_9SPHN Uncharacterized protein OS=Sphingomonas sp. IC081 OX=304378 GN=DM450_18440 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.81

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4786

0

4786

1556645

38

1716

325.2

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.369 ± 0.055

0.836 ± 0.012

5.8 ± 0.024

5.57 ± 0.033

3.499 ± 0.024

8.916 ± 0.035

2.035 ± 0.017

4.705 ± 0.022

2.975 ± 0.026

9.934 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.016

2.504 ± 0.024

5.271 ± 0.028

3.224 ± 0.021

7.341 ± 0.04

5.416 ± 0.027

5.296 ± 0.031

7.167 ± 0.027

1.452 ± 0.018

2.25 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski