Amino acid dipepetide frequency for Sphingomonas sp. IC081

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.568AlaAla: 19.568 ± 0.181
1.149AlaCys: 1.149 ± 0.031
7.334AlaAsp: 7.334 ± 0.075
7.855AlaGlu: 7.855 ± 0.083
4.282AlaPhe: 4.282 ± 0.058
11.392AlaGly: 11.392 ± 0.107
2.427AlaHis: 2.427 ± 0.044
6.369AlaIle: 6.369 ± 0.072
3.803AlaLys: 3.803 ± 0.063
14.233AlaLeu: 14.233 ± 0.134
4.043AlaMet: 4.043 ± 0.058
3.041AlaAsn: 3.041 ± 0.048
6.565AlaPro: 6.565 ± 0.078
4.805AlaGln: 4.805 ± 0.065
10.433AlaArg: 10.433 ± 0.09
7.114AlaSer: 7.114 ± 0.067
6.051AlaThr: 6.051 ± 0.066
8.827AlaVal: 8.827 ± 0.094
1.778AlaTrp: 1.778 ± 0.038
2.623AlaTyr: 2.623 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.074CysAla: 1.074 ± 0.024
0.094CysCys: 0.094 ± 0.009
0.525CysAsp: 0.525 ± 0.018
0.479CysGlu: 0.479 ± 0.018
0.294CysPhe: 0.294 ± 0.013
0.899CysGly: 0.899 ± 0.029
0.208CysHis: 0.208 ± 0.011
0.351CysIle: 0.351 ± 0.016
0.2CysLys: 0.2 ± 0.013
0.753CysLeu: 0.753 ± 0.021
0.16CysMet: 0.16 ± 0.012
0.233CysAsn: 0.233 ± 0.012
0.462CysPro: 0.462 ± 0.019
0.229CysGln: 0.229 ± 0.013
0.654CysArg: 0.654 ± 0.02
0.477CysSer: 0.477 ± 0.017
0.403CysThr: 0.403 ± 0.016
0.547CysVal: 0.547 ± 0.02
0.131CysTrp: 0.131 ± 0.009
0.185CysTyr: 0.185 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.289AspAla: 7.289 ± 0.074
0.51AspCys: 0.51 ± 0.018
3.129AspAsp: 3.129 ± 0.052
3.351AspGlu: 3.351 ± 0.05
2.153AspPhe: 2.153 ± 0.036
5.514AspGly: 5.514 ± 0.062
1.318AspHis: 1.318 ± 0.032
2.64AspIle: 2.64 ± 0.046
1.778AspLys: 1.778 ± 0.04
5.974AspLeu: 5.974 ± 0.063
1.378AspMet: 1.378 ± 0.033
1.396AspAsn: 1.396 ± 0.031
3.658AspPro: 3.658 ± 0.052
1.741AspGln: 1.741 ± 0.03
4.379AspArg: 4.379 ± 0.057
2.434AspSer: 2.434 ± 0.051
2.635AspThr: 2.635 ± 0.051
3.971AspVal: 3.971 ± 0.047
1.096AspTrp: 1.096 ± 0.026
1.662AspTyr: 1.662 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
8.159GluAla: 8.159 ± 0.08
0.391GluCys: 0.391 ± 0.017
2.849GluAsp: 2.849 ± 0.052
2.963GluGlu: 2.963 ± 0.058
1.639GluPhe: 1.639 ± 0.033
4.664GluGly: 4.664 ± 0.052
1.259GluHis: 1.259 ± 0.029
2.949GluIle: 2.949 ± 0.044
1.928GluLys: 1.928 ± 0.044
5.204GluLeu: 5.204 ± 0.067
1.442GluMet: 1.442 ± 0.03
1.424GluAsn: 1.424 ± 0.037
2.48GluPro: 2.48 ± 0.04
2.264GluGln: 2.264 ± 0.04
4.899GluArg: 4.899 ± 0.067
2.264GluSer: 2.264 ± 0.039
3.324GluThr: 3.324 ± 0.047
3.794GluVal: 3.794 ± 0.061
0.824GluTrp: 0.824 ± 0.023
0.978GluTyr: 0.978 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.831PheAla: 4.831 ± 0.06
0.331PheCys: 0.331 ± 0.014
2.66PheAsp: 2.66 ± 0.039
2.025PheGlu: 2.025 ± 0.034
1.16PhePhe: 1.16 ± 0.03
3.583PheGly: 3.583 ± 0.055
0.744PheHis: 0.744 ± 0.024
1.329PheIle: 1.329 ± 0.033
0.858PheLys: 0.858 ± 0.025
2.962PheLeu: 2.962 ± 0.044
0.755PheMet: 0.755 ± 0.021
1.006PheAsn: 1.006 ± 0.03
1.421PhePro: 1.421 ± 0.034
0.905PheGln: 0.905 ± 0.025
2.164PheArg: 2.164 ± 0.045
2.078PheSer: 2.078 ± 0.038
2.12PheThr: 2.12 ± 0.038
2.605PheVal: 2.605 ± 0.043
0.55PheTrp: 0.55 ± 0.022
0.903PheTyr: 0.903 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
9.969GlyAla: 9.969 ± 0.089
0.842GlyCys: 0.842 ± 0.024
4.866GlyAsp: 4.866 ± 0.061
5.21GlyGlu: 5.21 ± 0.063
3.574GlyPhe: 3.574 ± 0.048
8.107GlyGly: 8.107 ± 0.113
1.93GlyHis: 1.93 ± 0.038
4.245GlyIle: 4.245 ± 0.057
3.535GlyLys: 3.535 ± 0.051
8.832GlyLeu: 8.832 ± 0.081
2.417GlyMet: 2.417 ± 0.045
2.478GlyAsn: 2.478 ± 0.047
3.674GlyPro: 3.674 ± 0.05
3.094GlyGln: 3.094 ± 0.051
6.166GlyArg: 6.166 ± 0.071
5.101GlySer: 5.101 ± 0.078
5.042GlyThr: 5.042 ± 0.068
6.095GlyVal: 6.095 ± 0.059
1.587GlyTrp: 1.587 ± 0.034
2.399GlyTyr: 2.399 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.55HisAla: 2.55 ± 0.048
0.239HisCys: 0.239 ± 0.012
1.273HisAsp: 1.273 ± 0.029
1.086HisGlu: 1.086 ± 0.026
0.872HisPhe: 0.872 ± 0.024
2.053HisGly: 2.053 ± 0.04
0.576HisHis: 0.576 ± 0.024
0.824HisIle: 0.824 ± 0.024
0.49HisLys: 0.49 ± 0.017
1.909HisLeu: 1.909 ± 0.037
0.482HisMet: 0.482 ± 0.017
0.46HisAsn: 0.46 ± 0.02
1.255HisPro: 1.255 ± 0.031
0.547HisGln: 0.547 ± 0.021
1.467HisArg: 1.467 ± 0.032
0.959HisSer: 0.959 ± 0.027
0.773HisThr: 0.773 ± 0.023
1.552HisVal: 1.552 ± 0.032
0.382HisTrp: 0.382 ± 0.015
0.606HisTyr: 0.606 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.427IleAla: 7.427 ± 0.082
0.45IleCys: 0.45 ± 0.017
3.662IleAsp: 3.662 ± 0.051
3.425IleGlu: 3.425 ± 0.054
1.341IlePhe: 1.341 ± 0.032
4.844IleGly: 4.844 ± 0.059
0.86IleHis: 0.86 ± 0.027
1.605IleIle: 1.605 ± 0.036
1.169IleLys: 1.169 ± 0.032
3.573IleLeu: 3.573 ± 0.058
0.862IleMet: 0.862 ± 0.024
1.215IleAsn: 1.215 ± 0.029
2.212IlePro: 2.212 ± 0.042
1.125IleGln: 1.125 ± 0.028
3.017IleArg: 3.017 ± 0.048
2.558IleSer: 2.558 ± 0.044
2.443IleThr: 2.443 ± 0.035
3.712IleVal: 3.712 ± 0.053
0.572IleTrp: 0.572 ± 0.022
0.975IleTyr: 0.975 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.254LysAla: 4.254 ± 0.063
0.157LysCys: 0.157 ± 0.009
1.643LysAsp: 1.643 ± 0.036
1.255LysGlu: 1.255 ± 0.034
0.855LysPhe: 0.855 ± 0.027
2.694LysGly: 2.694 ± 0.048
0.506LysHis: 0.506 ± 0.019
1.413LysIle: 1.413 ± 0.033
0.996LysLys: 0.996 ± 0.029
3.062LysLeu: 3.062 ± 0.043
0.715LysMet: 0.715 ± 0.024
0.689LysAsn: 0.689 ± 0.024
1.978LysPro: 1.978 ± 0.042
0.922LysGln: 0.922 ± 0.025
2.09LysArg: 2.09 ± 0.04
1.557LysSer: 1.557 ± 0.031
1.679LysThr: 1.679 ± 0.035
2.316LysVal: 2.316 ± 0.042
0.386LysTrp: 0.386 ± 0.016
0.579LysTyr: 0.579 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.915LeuAla: 14.915 ± 0.136
0.865LeuCys: 0.865 ± 0.022
6.197LeuAsp: 6.197 ± 0.076
5.162LeuGlu: 5.162 ± 0.069
3.391LeuPhe: 3.391 ± 0.056
8.664LeuGly: 8.664 ± 0.086
1.858LeuHis: 1.858 ± 0.034
4.245LeuIle: 4.245 ± 0.06
2.876LeuLys: 2.876 ± 0.049
9.285LeuLeu: 9.285 ± 0.102
2.017LeuMet: 2.017 ± 0.032
2.191LeuAsn: 2.191 ± 0.041
5.701LeuPro: 5.701 ± 0.073
2.631LeuGln: 2.631 ± 0.041
7.151LeuArg: 7.151 ± 0.071
6.057LeuSer: 6.057 ± 0.076
5.583LeuThr: 5.583 ± 0.062
7.431LeuVal: 7.431 ± 0.084
1.172LeuTrp: 1.172 ± 0.031
1.949LeuTyr: 1.949 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
3.396MetAla: 3.396 ± 0.056
0.15MetCys: 0.15 ± 0.01
1.151MetAsp: 1.151 ± 0.027
1.102MetGlu: 1.102 ± 0.028
0.684MetPhe: 0.684 ± 0.023
1.885MetGly: 1.885 ± 0.035
0.436MetHis: 0.436 ± 0.015
1.265MetIle: 1.265 ± 0.03
0.898MetLys: 0.898 ± 0.025
2.625MetLeu: 2.625 ± 0.044
0.607MetMet: 0.607 ± 0.021
0.713MetAsn: 0.713 ± 0.022
1.499MetPro: 1.499 ± 0.03
0.779MetGln: 0.779 ± 0.022
1.832MetArg: 1.832 ± 0.034
1.443MetSer: 1.443 ± 0.028
1.774MetThr: 1.774 ± 0.036
1.688MetVal: 1.688 ± 0.034
0.211MetTrp: 0.211 ± 0.011
0.254MetTyr: 0.254 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.208AsnAla: 3.208 ± 0.05
0.251AsnCys: 0.251 ± 0.013
1.406AsnAsp: 1.406 ± 0.027
1.108AsnGlu: 1.108 ± 0.026
0.902AsnPhe: 0.902 ± 0.026
2.444AsnGly: 2.444 ± 0.05
0.483AsnHis: 0.483 ± 0.018
1.121AsnIle: 1.121 ± 0.025
0.587AsnLys: 0.587 ± 0.021
2.573AsnLeu: 2.573 ± 0.041
0.552AsnMet: 0.552 ± 0.019
0.639AsnAsn: 0.639 ± 0.027
1.738AsnPro: 1.738 ± 0.036
0.729AsnGln: 0.729 ± 0.022
1.857AsnArg: 1.857 ± 0.033
1.247AsnSer: 1.247 ± 0.031
1.249AsnThr: 1.249 ± 0.037
1.811AsnVal: 1.811 ± 0.034
0.421AsnTrp: 0.421 ± 0.018
0.709AsnTyr: 0.709 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.422ProAla: 7.422 ± 0.086
0.348ProCys: 0.348 ± 0.017
3.517ProAsp: 3.517 ± 0.048
3.625ProGlu: 3.625 ± 0.052
1.872ProPhe: 1.872 ± 0.038
4.837ProGly: 4.837 ± 0.058
1.018ProHis: 1.018 ± 0.028
2.295ProIle: 2.295 ± 0.045
1.456ProLys: 1.456 ± 0.032
4.974ProLeu: 4.974 ± 0.06
1.242ProMet: 1.242 ± 0.027
1.145ProAsn: 1.145 ± 0.024
2.629ProPro: 2.629 ± 0.059
1.792ProGln: 1.792 ± 0.03
3.19ProArg: 3.19 ± 0.051
2.773ProSer: 2.773 ± 0.037
2.437ProThr: 2.437 ± 0.043
4.374ProVal: 4.374 ± 0.055
0.719ProTrp: 0.719 ± 0.022
1.044ProTyr: 1.044 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.415GlnAla: 4.415 ± 0.059
0.237GlnCys: 0.237 ± 0.011
1.611GlnAsp: 1.611 ± 0.031
1.352GlnGlu: 1.352 ± 0.031
1.154GlnPhe: 1.154 ± 0.03
2.777GlnGly: 2.777 ± 0.044
0.655GlnHis: 0.655 ± 0.022
1.694GlnIle: 1.694 ± 0.035
0.932GlnLys: 0.932 ± 0.024
3.073GlnLeu: 3.073 ± 0.049
0.843GlnMet: 0.843 ± 0.023
0.793GlnAsn: 0.793 ± 0.027
1.779GlnPro: 1.779 ± 0.036
1.252GlnGln: 1.252 ± 0.032
2.512GlnArg: 2.512 ± 0.045
1.797GlnSer: 1.797 ± 0.036
1.686GlnThr: 1.686 ± 0.036
2.514GlnVal: 2.514 ± 0.04
0.506GlnTrp: 0.506 ± 0.018
0.653GlnTyr: 0.653 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.584ArgAla: 8.584 ± 0.086
0.522ArgCys: 0.522 ± 0.019
4.028ArgAsp: 4.028 ± 0.049
4.349ArgGlu: 4.349 ± 0.062
3.142ArgPhe: 3.142 ± 0.051
5.182ArgGly: 5.182 ± 0.072
1.837ArgHis: 1.837 ± 0.038
4.108ArgIle: 4.108 ± 0.055
2.44ArgLys: 2.44 ± 0.045
8.135ArgLeu: 8.135 ± 0.08
1.954ArgMet: 1.954 ± 0.04
1.785ArgAsn: 1.785 ± 0.035
3.588ArgPro: 3.588 ± 0.056
2.6ArgGln: 2.6 ± 0.042
5.902ArgArg: 5.902 ± 0.085
3.849ArgSer: 3.849 ± 0.051
3.59ArgThr: 3.59 ± 0.052
4.733ArgVal: 4.733 ± 0.053
1.19ArgTrp: 1.19 ± 0.029
1.887ArgTyr: 1.887 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.953SerAla: 6.953 ± 0.078
0.414SerCys: 0.414 ± 0.017
3.08SerAsp: 3.08 ± 0.051
2.789SerGlu: 2.789 ± 0.046
2.031SerPhe: 2.031 ± 0.042
5.757SerGly: 5.757 ± 0.064
1.041SerHis: 1.041 ± 0.025
2.503SerIle: 2.503 ± 0.041
1.444SerLys: 1.444 ± 0.034
5.324SerLeu: 5.324 ± 0.07
1.257SerMet: 1.257 ± 0.029
1.372SerAsn: 1.372 ± 0.034
2.944SerPro: 2.944 ± 0.049
1.765SerGln: 1.765 ± 0.035
3.792SerArg: 3.792 ± 0.054
3.046SerSer: 3.046 ± 0.063
2.735SerThr: 2.735 ± 0.049
3.707SerVal: 3.707 ± 0.047
0.806SerTrp: 0.806 ± 0.024
1.405SerTyr: 1.405 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.517ThrAla: 6.517 ± 0.068
0.421ThrCys: 0.421 ± 0.018
2.744ThrAsp: 2.744 ± 0.054
2.366ThrGlu: 2.366 ± 0.036
1.885ThrPhe: 1.885 ± 0.032
5.395ThrGly: 5.395 ± 0.063
0.951ThrHis: 0.951 ± 0.025
2.793ThrIle: 2.793 ± 0.04
1.2ThrLys: 1.2 ± 0.031
5.75ThrLeu: 5.75 ± 0.063
1.238ThrMet: 1.238 ± 0.032
1.278ThrAsn: 1.278 ± 0.031
3.318ThrPro: 3.318 ± 0.05
1.544ThrGln: 1.544 ± 0.04
3.601ThrArg: 3.601 ± 0.05
2.886ThrSer: 2.886 ± 0.046
2.802ThrThr: 2.802 ± 0.05
4.291ThrVal: 4.291 ± 0.055
0.674ThrTrp: 0.674 ± 0.019
1.308ThrTyr: 1.308 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
9.048ValAla: 9.048 ± 0.089
0.597ValCys: 0.597 ± 0.02
4.108ValAsp: 4.108 ± 0.051
4.388ValGlu: 4.388 ± 0.061
2.318ValPhe: 2.318 ± 0.04
5.283ValGly: 5.283 ± 0.062
1.381ValHis: 1.381 ± 0.035
3.745ValIle: 3.745 ± 0.05
2.029ValLys: 2.029 ± 0.033
7.15ValLeu: 7.15 ± 0.069
1.682ValMet: 1.682 ± 0.037
1.994ValAsn: 1.994 ± 0.042
4.043ValPro: 4.043 ± 0.052
2.115ValGln: 2.115 ± 0.04
5.044ValArg: 5.044 ± 0.057
4.368ValSer: 4.368 ± 0.044
4.693ValThr: 4.693 ± 0.057
5.374ValVal: 5.374 ± 0.07
0.92ValTrp: 0.92 ± 0.029
1.397ValTyr: 1.397 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.39TrpAla: 1.39 ± 0.031
0.145TrpCys: 0.145 ± 0.01
0.71TrpAsp: 0.71 ± 0.024
0.626TrpGlu: 0.626 ± 0.023
0.57TrpPhe: 0.57 ± 0.02
1.003TrpGly: 1.003 ± 0.026
0.389TrpHis: 0.389 ± 0.017
0.721TrpIle: 0.721 ± 0.025
0.531TrpLys: 0.531 ± 0.019
1.781TrpLeu: 1.781 ± 0.043
0.337TrpMet: 0.337 ± 0.014
0.513TrpAsn: 0.513 ± 0.019
0.722TrpPro: 0.722 ± 0.021
0.675TrpGln: 0.675 ± 0.022
1.317TrpArg: 1.317 ± 0.029
0.926TrpSer: 0.926 ± 0.026
0.813TrpThr: 0.813 ± 0.023
0.792TrpVal: 0.792 ± 0.026
0.24TrpTrp: 0.24 ± 0.012
0.321TrpTyr: 0.321 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.723TyrAla: 2.723 ± 0.041
0.246TyrCys: 0.246 ± 0.013
1.513TyrAsp: 1.513 ± 0.038
1.183TyrGlu: 1.183 ± 0.025
0.869TyrPhe: 0.869 ± 0.023
2.186TyrGly: 2.186 ± 0.041
0.478TyrHis: 0.478 ± 0.015
0.834TyrIle: 0.834 ± 0.024
0.596TyrLys: 0.596 ± 0.022
2.169TyrLeu: 2.169 ± 0.039
0.407TyrMet: 0.407 ± 0.018
0.668TyrAsn: 0.668 ± 0.026
1.044TyrPro: 1.044 ± 0.027
0.719TyrGln: 0.719 ± 0.023
1.939TyrArg: 1.939 ± 0.036
1.23TyrSer: 1.23 ± 0.033
1.133TyrThr: 1.133 ± 0.033
1.541TyrVal: 1.541 ± 0.031
0.358TyrTrp: 0.358 ± 0.016
0.662TyrTyr: 0.662 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4786 proteins (1556645 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski