Arsenicicoccus sp. MKL-02
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3030 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I3IWB5|A0A6I3IWB5_9MICO Rrf2 family transcriptional regulator OS=Arsenicicoccus sp. MKL-02 OX=2663847 GN=GGG17_04035 PE=4 SV=1
MM1 pKa = 7.23 SQTTTITVSGMTCGHH16 pKa = 6.36 CTSAVTSEE24 pKa = 4.05 LKK26 pKa = 10.19 EE27 pKa = 4.13 VQGVTDD33 pKa = 3.5 VQIDD37 pKa = 4.02 LVAGGDD43 pKa = 3.81 SPVTITSEE51 pKa = 4.53 SPVDD55 pKa = 3.41 PAAIEE60 pKa = 4.06 AAVDD64 pKa = 3.34 EE65 pKa = 4.72 AGYY68 pKa = 8.33 TVVKK72 pKa = 10.73
Molecular weight: 7.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A6I3ITJ4|A0A6I3ITJ4_9MICO Uncharacterized protein (Fragment) OS=Arsenicicoccus sp. MKL-02 OX=2663847 GN=GGG17_15080 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3030
0
3030
1004058
31
8153
331.4
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.106 ± 0.049
0.787 ± 0.012
6.343 ± 0.042
5.425 ± 0.054
2.433 ± 0.029
9.352 ± 0.041
2.347 ± 0.022
3.294 ± 0.032
1.885 ± 0.035
10.216 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.896 ± 0.022
1.559 ± 0.036
5.758 ± 0.036
3.014 ± 0.02
8.1 ± 0.066
5.216 ± 0.031
6.301 ± 0.095
9.498 ± 0.051
1.511 ± 0.022
1.959 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here