Myoviridae sp. ctThM1
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8L4|A0A5Q2W8L4_9CAUD Uncharacterized protein OS=Myoviridae sp. ctThM1 OX=2656730 PE=4 SV=1
MM1 pKa = 7.26 MSNIKK6 pKa = 9.84 QLLKK10 pKa = 10.6 QYY12 pKa = 10.7 EE13 pKa = 4.34 EE14 pKa = 4.51 VNAEE18 pKa = 4.04 LNKK21 pKa = 10.18 QIKK24 pKa = 10.37 DD25 pKa = 3.27 NGEE28 pKa = 3.74 AFIQEE33 pKa = 4.62 LFQDD37 pKa = 3.83 VFDD40 pKa = 3.88 NHH42 pKa = 6.47 QGLNVIGVVGWTMGFNDD59 pKa = 5.3 GEE61 pKa = 4.37 ACYY64 pKa = 9.06 HH65 pKa = 4.79 QQYY68 pKa = 10.29 IYY70 pKa = 10.63 TGVPAWKK77 pKa = 9.71 DD78 pKa = 3.38 YY79 pKa = 10.96 PDD81 pKa = 4.2 FYY83 pKa = 11.29 EE84 pKa = 4.04 EE85 pKa = 4.15 SGNFEE90 pKa = 4.04 EE91 pKa = 6.08 DD92 pKa = 3.55 FEE94 pKa = 5.23 FDD96 pKa = 3.48 EE97 pKa = 5.51 EE98 pKa = 4.49 EE99 pKa = 4.16 NKK101 pKa = 10.47 HH102 pKa = 6.35 LNSEE106 pKa = 4.34 CSTLQQAKK114 pKa = 9.86 AQVEE118 pKa = 4.41 AYY120 pKa = 10.42 DD121 pKa = 4.2 EE122 pKa = 4.5 IIEE125 pKa = 4.44 RR126 pKa = 11.84 VFGTNFRR133 pKa = 11.84 IVATRR138 pKa = 11.84 NEE140 pKa = 4.54 DD141 pKa = 2.99 NTAAVSVDD149 pKa = 3.88 EE150 pKa = 4.49 YY151 pKa = 11.37 DD152 pKa = 3.56 CGYY155 pKa = 11.34
Molecular weight: 17.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 4.101
IPC_protein 4.037
Toseland 3.859
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.859
Rodwell 3.872
Grimsley 3.77
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.24
Thurlkill 3.884
EMBOSS 3.872
Sillero 4.139
Patrickios 1.138
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.035
Protein with the highest isoelectric point:
>tr|A0A5Q2W386|A0A5Q2W386_9CAUD Uncharacterized protein OS=Myoviridae sp. ctThM1 OX=2656730 PE=4 SV=1
MM1 pKa = 7.7 ANILPILVVLPYY13 pKa = 10.08 VVSTIRR19 pKa = 11.84 YY20 pKa = 7.29 RR21 pKa = 11.84 SRR23 pKa = 11.84 SKK25 pKa = 10.55 IQVFQCFFKK34 pKa = 11.26 LFDD37 pKa = 3.88 TNKK40 pKa = 9.95 LRR42 pKa = 11.84 VFQLPITRR50 pKa = 11.84 FASKK54 pKa = 9.33 TLLFLSRR61 pKa = 11.84 NNCAIFVTIFATMAQTAMFCVYY83 pKa = 10.0 IFHH86 pKa = 7.48 LLPLKK91 pKa = 10.32 IYY93 pKa = 10.12 PKK95 pKa = 10.75
Molecular weight: 11.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.911
IPC_protein 10.423
Toseland 10.526
ProMoST 10.262
Dawson 10.672
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 11.096
Grimsley 10.73
Solomon 10.716
Lehninger 10.701
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.599
Patrickios 10.847
IPC_peptide 10.73
IPC2_peptide 9.472
IPC2.peptide.svr19 8.388
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
156
0
156
24069
50
934
154.3
17.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.498 ± 0.255
1.695 ± 0.15
6.012 ± 0.22
7.728 ± 0.266
4.429 ± 0.143
6.332 ± 0.223
1.762 ± 0.132
6.099 ± 0.172
7.449 ± 0.266
8.027 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.111
5.019 ± 0.145
3.207 ± 0.117
3.822 ± 0.169
4.404 ± 0.193
6.502 ± 0.186
5.65 ± 0.221
6.726 ± 0.179
1.6 ± 0.106
4.579 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here