Acidaminococcus intestini (strain RyC-MR95)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae; Acidaminococcus; Acidaminococcus intestini

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4Q5K9|G4Q5K9_ACIIR EstA_Ig_like domain-containing protein OS=Acidaminococcus intestini (strain RyC-MR95) OX=568816 GN=Acin_0883 PE=4 SV=1
MM1 pKa = 7.45EE2 pKa = 4.24KK3 pKa = 10.43VFRR6 pKa = 11.84KK7 pKa = 9.94FLASLFLSITMSIALEE23 pKa = 4.13ANAASLDD30 pKa = 3.73FSDD33 pKa = 4.67MYY35 pKa = 11.22SGASSEE41 pKa = 4.33GLVFSDD47 pKa = 4.51TLNSLNGSEE56 pKa = 4.06VTMTGFMAPPLKK68 pKa = 10.38PSINFFVLTEE78 pKa = 4.04TPMAVCPFCSTDD90 pKa = 4.05ADD92 pKa = 3.54WPYY95 pKa = 11.63NMVVVYY101 pKa = 10.42VNGSVDD107 pKa = 3.67ALPYY111 pKa = 9.59DD112 pKa = 3.52QEE114 pKa = 4.43VTVTGTLDD122 pKa = 3.1LGSYY126 pKa = 9.67MDD128 pKa = 4.73GDD130 pKa = 3.95TGFVSQVRR138 pKa = 11.84LLDD141 pKa = 3.62ATVEE145 pKa = 4.14

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4Q377|G4Q377_ACIIR Uncharacterized protein OS=Acidaminococcus intestini (strain RyC-MR95) OX=568816 GN=Acin_1669 PE=4 SV=1
MM1 pKa = 6.73VQQLRR6 pKa = 11.84ILLPLPPFVMTLIIGSLVNLSLVLAGRR33 pKa = 11.84FTAPAVIWAMSAALPAVAFMQGHH56 pKa = 6.97LALPFMIPVVFFSNAAYY73 pKa = 10.42AFFCKK78 pKa = 9.86EE79 pKa = 3.61AQRR82 pKa = 11.84ARR84 pKa = 11.84LRR86 pKa = 11.84ILIAPLFKK94 pKa = 10.53ASCMTAGLFAVSSLFQVGPQLVWRR118 pKa = 11.84FLLFYY123 pKa = 11.1GSMQWATSFLGCLFFEE139 pKa = 4.63LVARR143 pKa = 11.84RR144 pKa = 11.84LAGKK148 pKa = 10.29EE149 pKa = 4.12SVV151 pKa = 3.38

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2386

0

2386

708024

40

2578

296.7

32.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.848 ± 0.056

1.252 ± 0.02

5.484 ± 0.044

6.466 ± 0.058

4.109 ± 0.034

7.856 ± 0.054

2.198 ± 0.024

6.317 ± 0.047

6.381 ± 0.05

9.735 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.025

3.406 ± 0.044

4.072 ± 0.035

3.036 ± 0.026

5.044 ± 0.045

5.578 ± 0.039

5.643 ± 0.039

7.073 ± 0.043

0.951 ± 0.018

3.41 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski