Bifidobacterium boum

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086ZPA1|A0A086ZPA1_9BIFI Transporter major facilitator family protein OS=Bifidobacterium boum OX=78343 GN=BBOU_0481 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.56YY5 pKa = 10.0DD6 pKa = 3.94SPRR9 pKa = 11.84SKK11 pKa = 11.34DD12 pKa = 3.26EE13 pKa = 4.62DD14 pKa = 3.73EE15 pKa = 5.21EE16 pKa = 4.5SLQEE20 pKa = 4.7LGKK23 pKa = 9.06STQNTTNEE31 pKa = 4.42DD32 pKa = 3.5DD33 pKa = 4.57DD34 pKa = 4.7EE35 pKa = 4.37NAIAEE40 pKa = 4.35DD41 pKa = 4.23YY42 pKa = 9.11EE43 pKa = 5.05LPGADD48 pKa = 5.03LSNEE52 pKa = 4.1DD53 pKa = 3.77ASVTVIPMQGDD64 pKa = 3.48EE65 pKa = 5.44FICSEE70 pKa = 4.23CFLVKK75 pKa = 10.28HH76 pKa = 6.48RR77 pKa = 11.84SQLAYY82 pKa = 6.9TTADD86 pKa = 3.48GEE88 pKa = 4.65PVCKK92 pKa = 10.29EE93 pKa = 3.89CAAA96 pKa = 4.21

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086ZL01|A0A086ZL01_9BIFI R3H domain protein OS=Bifidobacterium boum OX=78343 GN=BBOU_1174 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.83MKK15 pKa = 9.27HH16 pKa = 5.56GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1726

0

1726

616755

29

3137

357.3

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.018 ± 0.07

1.008 ± 0.022

6.601 ± 0.045

5.247 ± 0.061

3.242 ± 0.039

7.858 ± 0.052

2.452 ± 0.03

5.467 ± 0.048

3.748 ± 0.058

8.517 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.026

3.244 ± 0.042

4.834 ± 0.044

3.813 ± 0.036

6.209 ± 0.064

6.176 ± 0.055

6.349 ± 0.05

7.575 ± 0.057

1.332 ± 0.022

2.713 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski