Peromyscus papillomavirus 1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1C9I5|G1C9I5_9PAPI Replication protein E1 OS=Peromyscus papillomavirus 1 OX=1074206 GN=E1 PE=3 SV=1
MM1 pKa = 7.65 IGPEE5 pKa = 3.64 ATLRR9 pKa = 11.84 DD10 pKa = 3.69 IVLEE14 pKa = 3.96 EE15 pKa = 4.5 SPPEE19 pKa = 4.16 TVSLYY24 pKa = 10.67 CDD26 pKa = 3.21 EE27 pKa = 4.43 TLSEE31 pKa = 4.25 EE32 pKa = 4.34 EE33 pKa = 4.3 EE34 pKa = 4.2 EE35 pKa = 4.66 EE36 pKa = 4.14 EE37 pKa = 4.67 AEE39 pKa = 4.32 SISPYY44 pKa = 10.02 RR45 pKa = 11.84 VYY47 pKa = 10.84 APCYY51 pKa = 8.31 RR52 pKa = 11.84 CDD54 pKa = 3.5 RR55 pKa = 11.84 SVRR58 pKa = 11.84 LVVSATEE65 pKa = 3.7 NGIRR69 pKa = 11.84 GLEE72 pKa = 3.92 KK73 pKa = 10.82 LLLGDD78 pKa = 3.7 VSIVCPSCAVCVRR91 pKa = 11.84 PTGHH95 pKa = 7.15 HH96 pKa = 6.58 GGQAA100 pKa = 3.07
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.2
IPC2_protein 4.482
IPC_protein 4.342
Toseland 4.215
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.101
Rodwell 4.19
Grimsley 4.126
Solomon 4.266
Lehninger 4.215
Nozaki 4.38
DTASelect 4.444
Thurlkill 4.202
EMBOSS 4.126
Sillero 4.457
Patrickios 1.036
IPC_peptide 4.279
IPC2_peptide 4.444
IPC2.peptide.svr19 4.372
Protein with the highest isoelectric point:
>tr|G1C9I7|G1C9I7_9PAPI Minor capsid protein L2 OS=Peromyscus papillomavirus 1 OX=1074206 GN=L2 PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.13 SLVSRR7 pKa = 11.84 LSVVQEE13 pKa = 4.54 EE14 pKa = 4.59 LMCMYY19 pKa = 10.48 EE20 pKa = 4.12 RR21 pKa = 11.84 GEE23 pKa = 4.29 DD24 pKa = 3.58 TLEE27 pKa = 4.08 SQVLHH32 pKa = 6.4 WSLIRR37 pKa = 11.84 KK38 pKa = 7.3 EE39 pKa = 4.07 QVLLHH44 pKa = 6.98 AARR47 pKa = 11.84 QHH49 pKa = 5.51 GLNKK53 pKa = 9.87 IGLQVVPPLGVTQTNAKK70 pKa = 9.71 NAIEE74 pKa = 3.87 MHH76 pKa = 6.4 LRR78 pKa = 11.84 LQSLANTPHH87 pKa = 6.94 AKK89 pKa = 10.26 EE90 pKa = 3.9 PWTLSEE96 pKa = 4.42 TSRR99 pKa = 11.84 EE100 pKa = 4.22 MYY102 pKa = 9.75 MSAPEE107 pKa = 3.83 QTFKK111 pKa = 11.15 KK112 pKa = 10.11 GGCTVHH118 pKa = 7.22 VYY120 pKa = 11.22 YY121 pKa = 10.93 DD122 pKa = 3.84 GNKK125 pKa = 8.17 EE126 pKa = 3.59 NAMQYY131 pKa = 9.46 VKK133 pKa = 10.21 WRR135 pKa = 11.84 FIYY138 pKa = 9.97 WLDD141 pKa = 4.82 DD142 pKa = 3.35 KK143 pKa = 11.47 GVWQRR148 pKa = 11.84 STSFSDD154 pKa = 2.98 HH155 pKa = 5.77 TGIYY159 pKa = 9.44 YY160 pKa = 10.41 EE161 pKa = 4.0 PGGRR165 pKa = 11.84 KK166 pKa = 7.42 TYY168 pKa = 10.87 YY169 pKa = 9.23 VTFAQDD175 pKa = 2.96 AARR178 pKa = 11.84 YY179 pKa = 6.77 STSSLWEE186 pKa = 3.76 VHH188 pKa = 6.92 DD189 pKa = 4.74 GPEE192 pKa = 3.39 IHH194 pKa = 6.86 LPVVPVTSSTPTSNPKK210 pKa = 9.65 RR211 pKa = 11.84 VAGIYY216 pKa = 9.86 PDD218 pKa = 4.42 GPPTTRR224 pKa = 11.84 PGLSSGDD231 pKa = 3.66 SSGHH235 pKa = 6.51 LDD237 pKa = 3.38 GLAGGGSDD245 pKa = 3.37 TCGGLQIPDD254 pKa = 3.96 SPSEE258 pKa = 4.22 RR259 pKa = 11.84 PTKK262 pKa = 10.28 RR263 pKa = 11.84 PRR265 pKa = 11.84 QRR267 pKa = 11.84 QPQDD271 pKa = 2.98 GQGPKK276 pKa = 9.21 RR277 pKa = 11.84 RR278 pKa = 11.84 KK279 pKa = 9.81 RR280 pKa = 11.84 EE281 pKa = 3.94 GTLKK285 pKa = 10.6 RR286 pKa = 11.84 IALGPRR292 pKa = 11.84 EE293 pKa = 4.24 TGEE296 pKa = 4.29 GAASRR301 pKa = 11.84 DD302 pKa = 3.42 PRR304 pKa = 11.84 TPTGPRR310 pKa = 11.84 PIPFTEE316 pKa = 4.54 EE317 pKa = 3.72 PSQGGGGPEE326 pKa = 4.11 SPGGPRR332 pKa = 11.84 SAPILSPAPHH342 pKa = 6.16 SVEE345 pKa = 3.69 TDD347 pKa = 3.32 GPAFGSQVGGQSQAPRR363 pKa = 11.84 RR364 pKa = 11.84 PRR366 pKa = 11.84 SPSPPSLLAEE376 pKa = 4.31 SAALPTASQLGQIAGGSQDD395 pKa = 3.39 PPPRR399 pKa = 11.84 RR400 pKa = 11.84 GRR402 pKa = 11.84 APRR405 pKa = 11.84 GQPGLPILLVCKK417 pKa = 9.78 GTANQVKK424 pKa = 9.82 CFRR427 pKa = 11.84 HH428 pKa = 5.37 KK429 pKa = 10.9 LKK431 pKa = 9.85 TVYY434 pKa = 9.86 PGCFDD439 pKa = 4.28 FCTTTWNWVDD449 pKa = 3.35 PSGSGTRR456 pKa = 11.84 MGRR459 pKa = 11.84 SRR461 pKa = 11.84 MLILCRR467 pKa = 11.84 DD468 pKa = 3.61 EE469 pKa = 4.12 QQYY472 pKa = 11.97 DD473 pKa = 3.5 MFTEE477 pKa = 4.42 EE478 pKa = 4.16 VKK480 pKa = 10.82 VPRR483 pKa = 11.84 GMQVDD488 pKa = 3.45 MCITHH493 pKa = 7.44 RR494 pKa = 11.84 LL495 pKa = 3.49
Molecular weight: 54.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.354
IPC2_protein 8.302
IPC_protein 8.214
Toseland 8.419
ProMoST 8.712
Dawson 8.99
Bjellqvist 9.107
Wikipedia 9.063
Rodwell 9.063
Grimsley 8.521
Solomon 9.092
Lehninger 9.063
Nozaki 9.253
DTASelect 8.916
Thurlkill 8.99
EMBOSS 9.151
Sillero 9.238
Patrickios 4.66
IPC_peptide 9.077
IPC2_peptide 8.024
IPC2.peptide.svr19 7.854
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2434
100
609
405.7
45.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.875 ± 0.24
2.219 ± 0.899
5.752 ± 0.524
6.286 ± 0.734
3.533 ± 0.542
8.628 ± 1.274
2.383 ± 0.155
4.437 ± 0.51
4.026 ± 0.749
7.354 ± 0.757
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.931 ± 0.419
3.246 ± 0.827
7.108 ± 1.062
4.15 ± 0.555
7.313 ± 0.338
8.094 ± 1.098
5.916 ± 0.514
7.025 ± 0.366
1.356 ± 0.354
3.369 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here