Lactococcus phage 38507

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus 38507

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343JP98|A0A343JP98_9CAUD Structural protein 4 OS=Lactococcus phage 38507 OX=2029663 GN=38507_11 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.5FDD4 pKa = 6.13SYY6 pKa = 11.42IDD8 pKa = 3.32WYY10 pKa = 11.58NNLLTMPLNDD20 pKa = 4.53IILGVKK26 pKa = 8.95DD27 pKa = 3.74TIEE30 pKa = 4.51DD31 pKa = 3.55KK32 pKa = 10.66TVYY35 pKa = 10.24LALSSSKK42 pKa = 9.95VVKK45 pKa = 9.81MDD47 pKa = 3.1NTSFIMGYY55 pKa = 9.09YY56 pKa = 7.66YY57 pKa = 10.47QVVLSVKK64 pKa = 10.59DD65 pKa = 3.57VDD67 pKa = 4.4DD68 pKa = 4.46KK69 pKa = 11.94LIGLVGNVLQDD80 pKa = 3.37GWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.45LPCGSGGQAWQQ112 pKa = 3.18

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343JPB8|A0A343JPB8_9CAUD Uncharacterized protein OS=Lactococcus phage 38507 OX=2029663 GN=38507_31 PE=4 SV=1
MM1 pKa = 7.68NNMCKK6 pKa = 9.79KK7 pKa = 10.35RR8 pKa = 11.84KK9 pKa = 5.77YY10 pKa = 8.39TKK12 pKa = 9.78MGALYY17 pKa = 10.41SIVSAQHH24 pKa = 5.58AKK26 pKa = 10.24KK27 pKa = 10.44SKK29 pKa = 10.17KK30 pKa = 9.93NKK32 pKa = 8.77NDD34 pKa = 4.1KK35 pKa = 10.66IPVRR39 pKa = 11.84AYY41 pKa = 8.07YY42 pKa = 9.98CKK44 pKa = 9.96WCACYY49 pKa = 9.97HH50 pKa = 6.65LSSQQRR56 pKa = 11.84LNIKK60 pKa = 9.21TGVIGG65 pKa = 3.97

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

9531

33

876

158.8

18.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.844 ± 0.484

0.86 ± 0.184

5.666 ± 0.289

7.733 ± 0.5

4.501 ± 0.299

6.075 ± 0.532

1.364 ± 0.201

7.124 ± 0.249

9.674 ± 0.511

8.614 ± 0.377

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.141

6.725 ± 0.241

2.014 ± 0.206

3.662 ± 0.205

3.662 ± 0.257

6.148 ± 0.5

6.033 ± 0.319

5.687 ± 0.313

1.437 ± 0.122

4.533 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski