Jimgerdemannia flammicorona
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13497 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A433DJH7|A0A433DJH7_9FUNG Uncharacterized protein OS=Jimgerdemannia flammicorona OX=994334 GN=BC936DRAFT_137279 PE=4 SV=1
MM1 pKa = 8.03 PLTLLNSPPSLEE13 pKa = 4.35 GEE15 pKa = 4.26 PIRR18 pKa = 11.84 YY19 pKa = 6.22 QQCDD23 pKa = 2.93 IRR25 pKa = 11.84 MVITPPLSGVSDD37 pKa = 3.54 AGKK40 pKa = 10.66 GNLVVAEE47 pKa = 4.21 SQLYY51 pKa = 9.52 FYY53 pKa = 11.0 SLSTTISVAIPYY65 pKa = 9.46 PSIIIHH71 pKa = 6.67 AISRR75 pKa = 11.84 QEE77 pKa = 3.9 GQPCVYY83 pKa = 9.93 CQLDD87 pKa = 3.66 STEE90 pKa = 4.2 KK91 pKa = 10.62 LVEE94 pKa = 3.94 QRR96 pKa = 11.84 HH97 pKa = 5.57 GGRR100 pKa = 11.84 DD101 pKa = 3.69 DD102 pKa = 3.76 EE103 pKa = 6.56 DD104 pKa = 4.06 EE105 pKa = 5.8 DD106 pKa = 4.14 EE107 pKa = 5.89 DD108 pKa = 4.16 EE109 pKa = 4.2 VTEE112 pKa = 4.37 IRR114 pKa = 11.84 FVPDD118 pKa = 3.23 DD119 pKa = 4.29 AGALEE124 pKa = 4.6 YY125 pKa = 10.14 IYY127 pKa = 10.45 IALSEE132 pKa = 4.34 CAALHH137 pKa = 6.34 PDD139 pKa = 3.35 KK140 pKa = 11.22 EE141 pKa = 4.44 FMVDD145 pKa = 3.43 QLEE148 pKa = 4.54 DD149 pKa = 3.6 EE150 pKa = 4.87 HH151 pKa = 9.05 DD152 pKa = 4.05 GGADD156 pKa = 3.68 DD157 pKa = 6.37 DD158 pKa = 5.75 DD159 pKa = 6.83 GYY161 pKa = 11.46 DD162 pKa = 3.78 YY163 pKa = 11.36 DD164 pKa = 5.17 NPAADD169 pKa = 4.62 TYY171 pKa = 10.64 TPSDD175 pKa = 3.58 PAEE178 pKa = 4.04 LSEE181 pKa = 4.33 VGRR184 pKa = 11.84 AALAHH189 pKa = 6.28 LNSVFQMPSQRR200 pKa = 11.84 DD201 pKa = 3.78 FEE203 pKa = 4.62 QMVAEE208 pKa = 4.39 NGHH211 pKa = 5.45 TNGVSDD217 pKa = 4.29 EE218 pKa = 4.4 RR219 pKa = 11.84 FGDD222 pKa = 3.8 AEE224 pKa = 4.2 EE225 pKa = 4.41 QGGSNN230 pKa = 3.87
Molecular weight: 25.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.681
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.719
EMBOSS 3.783
Sillero 3.999
Patrickios 1.252
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A433A2W2|A0A433A2W2_9FUNG Uncharacterized protein OS=Jimgerdemannia flammicorona OX=994334 GN=BC936DRAFT_141107 PE=4 SV=1
MM1 pKa = 7.86 PLPRR5 pKa = 11.84 LQLLPLRR12 pKa = 11.84 PRR14 pKa = 11.84 PRR16 pKa = 11.84 PRR18 pKa = 11.84 PRR20 pKa = 11.84 PRR22 pKa = 11.84 PRR24 pKa = 11.84 ARR26 pKa = 11.84 LRR28 pKa = 11.84 PRR30 pKa = 11.84 LRR32 pKa = 11.84 PRR34 pKa = 11.84 LRR36 pKa = 11.84 PRR38 pKa = 11.84 LRR40 pKa = 11.84 PRR42 pKa = 11.84 LRR44 pKa = 11.84 TRR46 pKa = 11.84 LRR48 pKa = 11.84 PRR50 pKa = 11.84 LRR52 pKa = 11.84 PRR54 pKa = 11.84 LRR56 pKa = 11.84 NRR58 pKa = 11.84 LRR60 pKa = 11.84 PRR62 pKa = 11.84 LQNRR66 pKa = 11.84 LCPRR70 pKa = 11.84 LRR72 pKa = 11.84 NRR74 pKa = 11.84 LRR76 pKa = 11.84 PRR78 pKa = 11.84 LQNRR82 pKa = 11.84 LCPRR86 pKa = 11.84 LRR88 pKa = 11.84 NRR90 pKa = 11.84 LRR92 pKa = 11.84 PRR94 pKa = 11.84 LRR96 pKa = 11.84 SQPFPLRR103 pKa = 11.84 HH104 pKa = 5.81 HH105 pKa = 6.34 QPLPLLLL112 pKa = 4.9
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.51
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.274
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13120
377
13497
4714404
8
5143
349.3
38.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.85 ± 0.019
1.516 ± 0.012
5.566 ± 0.017
6.044 ± 0.026
3.795 ± 0.014
6.494 ± 0.027
2.726 ± 0.011
5.136 ± 0.018
5.008 ± 0.023
9.046 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.01
3.963 ± 0.016
5.89 ± 0.028
3.749 ± 0.021
6.264 ± 0.021
7.697 ± 0.025
6.21 ± 0.017
6.59 ± 0.019
1.314 ± 0.008
2.771 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here