Streptomyces lydicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7VR16|A0A1D7VR16_9ACTN Uncharacterized protein OS=Streptomyces lydicus OX=47763 GN=SL103_25595 PE=4 SV=1
MM1 pKa = 7.73PEE3 pKa = 3.37VTAAYY8 pKa = 10.14QPGTPCWVDD17 pKa = 3.59LAAPDD22 pKa = 3.44QQAAIDD28 pKa = 4.64FYY30 pKa = 11.23SQVFGWTGEE39 pKa = 3.86IGPPEE44 pKa = 4.03TGGYY48 pKa = 9.26AVCLLNGKK56 pKa = 8.31PVAGIMAAMAMGGQPPPPTVWTTYY80 pKa = 10.3LAAHH84 pKa = 7.06DD85 pKa = 4.6ADD87 pKa = 4.01AAAQAVSSAGGTILMPVMDD106 pKa = 4.46VMTLGRR112 pKa = 11.84MCIAGDD118 pKa = 3.3PTGAVFGIWQAVDD131 pKa = 3.73FPGAGIVNEE140 pKa = 4.19PGALIWNEE148 pKa = 4.02LNTTEE153 pKa = 4.54PSAGAAFYY161 pKa = 10.45KK162 pKa = 10.46AALGIEE168 pKa = 4.28SAPIEE173 pKa = 4.47GADD176 pKa = 3.11NYY178 pKa = 11.18YY179 pKa = 10.94ALKK182 pKa = 11.12VNDD185 pKa = 4.03RR186 pKa = 11.84PVGGMQPMSEE196 pKa = 4.22QMPPDD201 pKa = 4.17TPSHH205 pKa = 5.54WLAYY209 pKa = 9.68FAVADD214 pKa = 3.95TDD216 pKa = 3.66EE217 pKa = 4.51TVAKK221 pKa = 8.97VTAAGGAALQQPFDD235 pKa = 3.67MMAGRR240 pKa = 11.84MAVVTDD246 pKa = 3.74PQGATFAVINPKK258 pKa = 9.7PLQGG262 pKa = 3.54

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D4DQU7|A0A0D4DQU7_9ACTN 30S ribosomal protein S13 OS=Streptomyces lydicus OX=47763 GN=rpsM PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6848

0

6848

2258271

29

6152

329.8

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.195 ± 0.043

0.814 ± 0.009

5.845 ± 0.024

5.487 ± 0.032

2.712 ± 0.016

9.676 ± 0.03

2.396 ± 0.016

3.014 ± 0.022

2.068 ± 0.03

10.542 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.013

1.693 ± 0.015

6.3 ± 0.031

2.752 ± 0.02

8.227 ± 0.037

4.768 ± 0.019

6.03 ± 0.033

8.281 ± 0.03

1.487 ± 0.014

2.01 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski