Moraxella lincolnii
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1751 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T0CF43|A0A1T0CF43_9GAMM Osmotically inducible protein OsmC OS=Moraxella lincolnii OX=90241 GN=B0682_05245 PE=3 SV=1
MM1 pKa = 7.52 KK2 pKa = 9.8 LKK4 pKa = 10.76 SSIVFAMLTLLSLTACDD21 pKa = 4.66 NSNKK25 pKa = 10.22 SNADD29 pKa = 3.32 QSSTTQQATHH39 pKa = 6.69 EE40 pKa = 4.36 EE41 pKa = 4.4 ASTQAVEE48 pKa = 4.26 QPIIDD53 pKa = 3.97 STDD56 pKa = 3.3 DD57 pKa = 3.51 AVADD61 pKa = 5.17 PIDD64 pKa = 5.47 DD65 pKa = 4.19 GQQPEE70 pKa = 4.41 VTVTDD75 pKa = 4.28 GVGVLPQSAYY85 pKa = 11.09 SGVLPCADD93 pKa = 3.58 CSGIQTDD100 pKa = 4.04 LTLNADD106 pKa = 3.55 GTFVMEE112 pKa = 4.33 QEE114 pKa = 4.43 YY115 pKa = 10.68 LGKK118 pKa = 9.45 PDD120 pKa = 3.76 GQITTKK126 pKa = 9.35 GTYY129 pKa = 9.81 DD130 pKa = 3.29 INGLDD135 pKa = 3.23 SRR137 pKa = 11.84 YY138 pKa = 10.49 VLLHH142 pKa = 6.99 PEE144 pKa = 4.53 DD145 pKa = 4.08 QSDD148 pKa = 3.55 IPPYY152 pKa = 10.03 LIYY155 pKa = 9.65 MDD157 pKa = 4.29 KK158 pKa = 11.16 DD159 pKa = 3.62 SVQFRR164 pKa = 11.84 DD165 pKa = 3.32 IEE167 pKa = 4.37 NGEE170 pKa = 4.32 EE171 pKa = 3.98 PSPDD175 pKa = 2.93 HH176 pKa = 6.13 TLNLVSS182 pKa = 4.37
Molecular weight: 19.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A1T0CJP7|A0A1T0CJP7_9GAMM Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Moraxella lincolnii OX=90241 GN=mraY PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSIIKK11 pKa = 10.36 RR12 pKa = 11.84 KK13 pKa = 7.45 RR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.32 KK26 pKa = 10.31 GRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1751
0
1751
579145
26
2452
330.8
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.466 ± 0.063
0.921 ± 0.019
6.181 ± 0.054
4.656 ± 0.056
3.685 ± 0.038
6.541 ± 0.058
2.914 ± 0.037
6.499 ± 0.042
5.541 ± 0.051
10.179 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.85 ± 0.031
5.017 ± 0.053
3.962 ± 0.034
5.607 ± 0.067
4.168 ± 0.045
6.054 ± 0.04
5.944 ± 0.039
6.672 ± 0.052
1.158 ± 0.025
2.986 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here