Gordonia phage Schnabeltier
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142KA17|A0A142KA17_9CAUD Uncharacterized protein OS=Gordonia phage Schnabeltier OX=1821561 GN=29 PE=4 SV=1
MM1 pKa = 6.78 TTPPPTFTLPEE12 pKa = 4.26 YY13 pKa = 10.33 PGVTYY18 pKa = 9.85 TVLTGSGGVPGLPSNAVRR36 pKa = 11.84 IVGTVVNPWHH46 pKa = 6.14 EE47 pKa = 3.88 PEE49 pKa = 4.27 YY50 pKa = 11.2 NYY52 pKa = 11.36 GLDD55 pKa = 3.51 PNGYY59 pKa = 7.8 TEE61 pKa = 5.28 VTDD64 pKa = 3.13 PWKK67 pKa = 10.71 RR68 pKa = 11.84 HH69 pKa = 4.33 TEE71 pKa = 4.27 LIEE74 pKa = 3.91 VTNTGFLAPTAPVPTEE90 pKa = 4.34 PPPSPPAPTPDD101 pKa = 3.85 PEE103 pKa = 4.27 PTPEE107 pKa = 3.9 EE108 pKa = 4.23 VV109 pKa = 3.16
Molecular weight: 11.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.964
IPC2_protein 4.24
IPC_protein 4.075
Toseland 3.935
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.177
Wikipedia 3.872
Rodwell 3.923
Grimsley 3.859
Solomon 3.999
Lehninger 3.948
Nozaki 4.139
DTASelect 4.202
Thurlkill 3.961
EMBOSS 3.897
Sillero 4.19
Patrickios 0.769
IPC_peptide 4.012
IPC2_peptide 4.177
IPC2.peptide.svr19 4.073
Protein with the highest isoelectric point:
>tr|A0A142KA40|A0A142KA40_9CAUD Uncharacterized protein OS=Gordonia phage Schnabeltier OX=1821561 GN=52 PE=4 SV=1
MM1 pKa = 7.19 ARR3 pKa = 11.84 IRR5 pKa = 11.84 ARR7 pKa = 11.84 LTALLIAAVAAATLSACDD25 pKa = 3.38 TDD27 pKa = 5.81 GYY29 pKa = 9.2 TEE31 pKa = 4.03 PVSNEE36 pKa = 3.72 VCVDD40 pKa = 3.2 RR41 pKa = 11.84 SDD43 pKa = 5.57 RR44 pKa = 11.84 RR45 pKa = 11.84 AQDD48 pKa = 3.6 HH49 pKa = 5.73 YY50 pKa = 11.18 CEE52 pKa = 4.64 SGFPNYY58 pKa = 9.48 HH59 pKa = 4.86 WWYY62 pKa = 9.77 LPAGTYY68 pKa = 10.33 VGIGQHH74 pKa = 4.81 VTGGTNLRR82 pKa = 11.84 PNRR85 pKa = 11.84 VRR87 pKa = 11.84 RR88 pKa = 11.84 APTVTVTKK96 pKa = 9.32 KK97 pKa = 5.53 TTPRR101 pKa = 11.84 TTAATPRR108 pKa = 11.84 KK109 pKa = 7.56 PTTKK113 pKa = 9.51 STTTKK118 pKa = 9.59 TRR120 pKa = 11.84 TRR122 pKa = 11.84 TTRR125 pKa = 2.97
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.794
IPC_protein 10.716
Toseland 10.599
ProMoST 10.643
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.862
Grimsley 10.804
Solomon 10.862
Lehninger 10.818
Nozaki 10.613
DTASelect 10.496
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.672
Patrickios 10.57
IPC_peptide 10.862
IPC2_peptide 9.721
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
15240
43
1704
214.6
23.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.579 ± 0.515
0.866 ± 0.134
6.424 ± 0.309
5.61 ± 0.346
2.71 ± 0.208
8.484 ± 0.427
1.995 ± 0.171
4.469 ± 0.247
2.946 ± 0.188
7.992 ± 0.288
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.211 ± 0.162
2.749 ± 0.189
6.24 ± 0.319
3.412 ± 0.175
7.598 ± 0.361
5.781 ± 0.192
6.358 ± 0.26
7.434 ± 0.295
2.152 ± 0.146
1.988 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here