Prevotella sp. P3-120

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255S836|A0A255S836_9BACT PSP1 C-terminal domain-containing protein OS=Prevotella sp. P3-120 OX=2024220 GN=CIK97_08680 PE=4 SV=1
MM1 pKa = 7.25TSIEE5 pKa = 4.43EE6 pKa = 4.03EE7 pKa = 3.97LLMDD11 pKa = 4.32EE12 pKa = 4.83EE13 pKa = 4.79NDD15 pKa = 3.42ARR17 pKa = 11.84EE18 pKa = 4.18AEE20 pKa = 4.65YY21 pKa = 10.42ILHH24 pKa = 6.05TLSSEE29 pKa = 3.9LQDD32 pKa = 4.99RR33 pKa = 11.84YY34 pKa = 7.63TTDD37 pKa = 4.71DD38 pKa = 3.4IRR40 pKa = 11.84TMMDD44 pKa = 4.4LIVEE48 pKa = 4.25YY49 pKa = 10.58YY50 pKa = 9.66YY51 pKa = 11.13DD52 pKa = 4.2SGMLEE57 pKa = 4.1CTDD60 pKa = 3.95AEE62 pKa = 4.55VEE64 pKa = 4.38IDD66 pKa = 3.77LQDD69 pKa = 3.51VADD72 pKa = 4.23YY73 pKa = 10.14VAKK76 pKa = 10.17HH77 pKa = 5.42AKK79 pKa = 9.33EE80 pKa = 4.02YY81 pKa = 10.62HH82 pKa = 6.02EE83 pKa = 4.64KK84 pKa = 10.34DD85 pKa = 3.73YY86 pKa = 11.11PVEE89 pKa = 4.1DD90 pKa = 3.51VFFVVQADD98 pKa = 4.26FDD100 pKa = 3.91WQEE103 pKa = 3.7QNLL106 pKa = 3.8

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255S6V6|A0A255S6V6_9BACT SusC/RagA family protein OS=Prevotella sp. P3-120 OX=2024220 GN=CIK97_09415 PE=3 SV=1
MM1 pKa = 7.24KK2 pKa = 9.44QTITLNRR9 pKa = 11.84NEE11 pKa = 4.11RR12 pKa = 11.84LMLNVQRR19 pKa = 11.84IANLANKK26 pKa = 9.12PLAALANYY34 pKa = 8.96YY35 pKa = 10.7SRR37 pKa = 11.84LLGEE41 pKa = 4.44KK42 pKa = 10.14VNVRR46 pKa = 11.84QTLLLCNAQAAFFFLVFASMSLVLRR71 pKa = 11.84LLCLLWFCNALLKK84 pKa = 10.68CRR86 pKa = 11.84NSGLGRR92 pKa = 11.84DD93 pKa = 3.63

Molecular weight: 10.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2554

0

2554

919724

38

2439

360.1

40.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.461 ± 0.045

1.418 ± 0.017

5.951 ± 0.032

6.186 ± 0.047

4.232 ± 0.03

6.697 ± 0.044

2.284 ± 0.022

6.673 ± 0.05

6.253 ± 0.047

8.777 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.057 ± 0.024

4.873 ± 0.038

3.638 ± 0.027

3.947 ± 0.032

4.995 ± 0.039

5.702 ± 0.04

5.711 ± 0.037

6.626 ± 0.037

1.32 ± 0.02

4.197 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski