Sphaerisporangium album

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Sphaerisporangium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367FPH2|A0A367FPH2_9ACTN Uncharacterized protein OS=Sphaerisporangium album OX=509200 GN=DQ384_07280 PE=4 SV=1
MM1 pKa = 6.94TTQFWTCVLDD11 pKa = 3.56WSSSHH16 pKa = 6.71GDD18 pKa = 3.63LDD20 pKa = 3.93PPVCVVQAEE29 pKa = 4.65DD30 pKa = 4.24PDD32 pKa = 4.22SATDD36 pKa = 3.49QAISLTSDD44 pKa = 3.06HH45 pKa = 6.76FVGIDD50 pKa = 3.89SDD52 pKa = 3.77WKK54 pKa = 10.73SADD57 pKa = 3.73DD58 pKa = 3.51VAEE61 pKa = 4.0QIFPIVTFHH70 pKa = 6.93GDD72 pKa = 2.78ISGLIASPGHH82 pKa = 6.06YY83 pKa = 9.49ILRR86 pKa = 11.84GPGLL90 pKa = 3.56

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367F4Y2|A0A367F4Y2_9ACTN Nitrogen regulation protein B OS=Sphaerisporangium album OX=509200 GN=DQ384_31765 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PLSPPPRR12 pKa = 11.84ASPAPPRR19 pKa = 11.84PRR21 pKa = 11.84IQAPRR26 pKa = 11.84PRR28 pKa = 11.84PPPSPRR34 pKa = 11.84CPPRR38 pKa = 11.84RR39 pKa = 11.84TPPPSRR45 pKa = 11.84RR46 pKa = 11.84PRR48 pKa = 11.84PPTPPTMRR56 pKa = 11.84TTQTTRR62 pKa = 11.84TMRR65 pKa = 11.84SRR67 pKa = 11.84APPRR71 pKa = 11.84RR72 pKa = 11.84PRR74 pKa = 11.84ASPAAPRR81 pKa = 11.84ARR83 pKa = 3.55

Molecular weight: 9.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7626

0

7626

2573401

21

8283

337.5

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.228 ± 0.04

0.797 ± 0.009

5.84 ± 0.023

5.479 ± 0.032

2.8 ± 0.017

9.52 ± 0.027

2.197 ± 0.015

3.553 ± 0.019

2.03 ± 0.022

10.144 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.009

1.891 ± 0.022

6.236 ± 0.024

2.626 ± 0.019

8.259 ± 0.039

5.142 ± 0.021

6.029 ± 0.032

8.647 ± 0.026

1.555 ± 0.012

2.195 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski