Amino acid dipepetide frequency for Sphaerisporangium album

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.772AlaAla: 19.772 ± 0.151	1.123AlaCys: 1.123 ± 0.025	7.618AlaAsp: 7.618 ± 0.053	8.174AlaGlu: 8.174 ± 0.08	3.589AlaPhe: 3.589 ± 0.04	13.473AlaGly: 13.473 ± 0.092	2.611AlaHis: 2.611 ± 0.035	4.297AlaIle: 4.297 ± 0.048	2.682AlaLys: 2.682 ± 0.043	13.585AlaLeu: 13.585 ± 0.098	2.631AlaMet: 2.631 ± 0.037	2.159AlaAsn: 2.159 ± 0.035	6.627AlaPro: 6.627 ± 0.066	3.39AlaGln: 3.39 ± 0.046	10.721AlaArg: 10.721 ± 0.093	6.344AlaSer: 6.344 ± 0.053	7.263AlaThr: 7.263 ± 0.068	11.52AlaVal: 11.52 ± 0.086	1.911AlaTrp: 1.911 ± 0.03	2.786AlaTyr: 2.786 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.055CysAla: 1.055 ± 0.019	0.095CysCys: 0.095 ± 0.006	0.514CysAsp: 0.514 ± 0.015	0.417CysGlu: 0.417 ± 0.014	0.21CysPhe: 0.21 ± 0.009	0.96CysGly: 0.96 ± 0.025	0.214CysHis: 0.214 ± 0.01	0.137CysIle: 0.137 ± 0.007	0.129CysLys: 0.129 ± 0.008	0.778CysLeu: 0.778 ± 0.019	0.113CysMet: 0.113 ± 0.007	0.155CysAsn: 0.155 ± 0.009	0.509CysPro: 0.509 ± 0.014	0.17CysGln: 0.17 ± 0.008	0.635CysArg: 0.635 ± 0.017	0.431CysSer: 0.431 ± 0.012	0.429CysThr: 0.429 ± 0.014	0.708CysVal: 0.708 ± 0.015	0.125CysTrp: 0.125 ± 0.007	0.189CysTyr: 0.189 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.203AspAla: 7.203 ± 0.064	0.412AspCys: 0.412 ± 0.015	3.563AspAsp: 3.563 ± 0.037	3.627AspGlu: 3.627 ± 0.046	1.578AspPhe: 1.578 ± 0.027	6.03AspGly: 6.03 ± 0.055	1.448AspHis: 1.448 ± 0.025	1.89AspIle: 1.89 ± 0.028	1.093AspLys: 1.093 ± 0.028	6.547AspLeu: 6.547 ± 0.05	0.848AspMet: 0.848 ± 0.02	0.967AspAsn: 0.967 ± 0.024	4.653AspPro: 4.653 ± 0.046	1.651AspGln: 1.651 ± 0.03	4.799AspArg: 4.799 ± 0.045	2.217AspSer: 2.217 ± 0.03	2.736AspThr: 2.736 ± 0.035	5.002AspVal: 5.002 ± 0.047	0.938AspTrp: 0.938 ± 0.021	1.202AspTyr: 1.202 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	7.011GluAla: 7.011 ± 0.065	0.354GluCys: 0.354 ± 0.014	2.771GluAsp: 2.771 ± 0.037	3.252GluGlu: 3.252 ± 0.048	1.471GluPhe: 1.471 ± 0.023	4.087GluGly: 4.087 ± 0.047	1.453GluHis: 1.453 ± 0.023	2.471GluIle: 2.471 ± 0.032	1.198GluLys: 1.198 ± 0.027	6.325GluLeu: 6.325 ± 0.058	0.964GluMet: 0.964 ± 0.02	1.008GluAsn: 1.008 ± 0.022	3.261GluPro: 3.261 ± 0.042	1.892GluGln: 1.892 ± 0.029	5.374GluArg: 5.374 ± 0.062	2.454GluSer: 2.454 ± 0.03	2.801GluThr: 2.801 ± 0.035	4.733GluVal: 4.733 ± 0.048	0.807GluTrp: 0.807 ± 0.019	1.104GluTyr: 1.104 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.7PheAla: 3.7 ± 0.043	0.272PheCys: 0.272 ± 0.011	2.003PheAsp: 2.003 ± 0.031	1.451PheGlu: 1.451 ± 0.027	0.852PhePhe: 0.852 ± 0.021	3.057PheGly: 3.057 ± 0.036	0.626PheHis: 0.626 ± 0.015	0.762PheIle: 0.762 ± 0.016	0.516PheLys: 0.516 ± 0.016	2.576PheLeu: 2.576 ± 0.037	0.449PheMet: 0.449 ± 0.014	0.616PheAsn: 0.616 ± 0.017	1.467PhePro: 1.467 ± 0.028	0.723PheGln: 0.723 ± 0.015	1.87PheArg: 1.87 ± 0.027	1.544PheSer: 1.544 ± 0.029	2.108PheThr: 2.108 ± 0.034	2.313PheVal: 2.313 ± 0.031	0.446PheTrp: 0.446 ± 0.013	0.651PheTyr: 0.651 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.718GlyAla: 10.718 ± 0.071	0.821GlyCys: 0.821 ± 0.02	5.603GlyAsp: 5.603 ± 0.049	5.259GlyGlu: 5.259 ± 0.047	2.956GlyPhe: 2.956 ± 0.037	9.131GlyGly: 9.131 ± 0.09	2.407GlyHis: 2.407 ± 0.033	3.222GlyIle: 3.222 ± 0.037	2.297GlyLys: 2.297 ± 0.04	9.457GlyLeu: 9.457 ± 0.079	1.947GlyMet: 1.947 ± 0.03	1.911GlyAsn: 1.911 ± 0.042	5.322GlyPro: 5.322 ± 0.053	2.653GlyGln: 2.653 ± 0.036	8.227GlyArg: 8.227 ± 0.068	5.028GlySer: 5.028 ± 0.047	6.081GlyThr: 6.081 ± 0.068	8.099GlyVal: 8.099 ± 0.06	1.671GlyTrp: 1.671 ± 0.029	2.386GlyTyr: 2.386 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.655HisAla: 2.655 ± 0.032	0.19HisCys: 0.19 ± 0.008	1.423HisAsp: 1.423 ± 0.028	1.165HisGlu: 1.165 ± 0.021	0.606HisPhe: 0.606 ± 0.013	2.194HisGly: 2.194 ± 0.031	0.644HisHis: 0.644 ± 0.02	0.703HisIle: 0.703 ± 0.018	0.318HisLys: 0.318 ± 0.012	2.404HisLeu: 2.404 ± 0.032	0.361HisMet: 0.361 ± 0.013	0.38HisAsn: 0.38 ± 0.013	1.717HisPro: 1.717 ± 0.026	0.591HisGln: 0.591 ± 0.016	1.918HisArg: 1.918 ± 0.032	0.905HisSer: 0.905 ± 0.018	1.16HisThr: 1.16 ± 0.022	1.815HisVal: 1.815 ± 0.03	0.344HisTrp: 0.344 ± 0.011	0.479HisTyr: 0.479 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.04IleAla: 5.04 ± 0.048	0.314IleCys: 0.314 ± 0.013	2.436IleAsp: 2.436 ± 0.035	2.105IleGlu: 2.105 ± 0.033	0.886IlePhe: 0.886 ± 0.02	3.777IleGly: 3.777 ± 0.04	0.65IleHis: 0.65 ± 0.018	1.169IleIle: 1.169 ± 0.022	0.759IleLys: 0.759 ± 0.02	2.815IleLeu: 2.815 ± 0.04	0.584IleMet: 0.584 ± 0.015	0.795IleAsn: 0.795 ± 0.02	2.017IlePro: 2.017 ± 0.028	0.827IleGln: 0.827 ± 0.018	2.527IleArg: 2.527 ± 0.033	1.969IleSer: 1.969 ± 0.029	2.496IleThr: 2.496 ± 0.035	3.18IleVal: 3.18 ± 0.042	0.472IleTrp: 0.472 ± 0.014	0.708IleTyr: 0.708 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.649LysAla: 2.649 ± 0.043	0.133LysCys: 0.133 ± 0.008	1.162LysAsp: 1.162 ± 0.03	1.073LysGlu: 1.073 ± 0.022	0.462LysPhe: 0.462 ± 0.012	1.734LysGly: 1.734 ± 0.029	0.394LysHis: 0.394 ± 0.012	1.054LysIle: 1.054 ± 0.022	0.625LysLys: 0.625 ± 0.021	1.925LysLeu: 1.925 ± 0.029	0.373LysMet: 0.373 ± 0.014	0.492LysAsn: 0.492 ± 0.017	1.331LysPro: 1.331 ± 0.026	0.6LysGln: 0.6 ± 0.017	1.381LysArg: 1.381 ± 0.029	1.052LysSer: 1.052 ± 0.022	1.229LysThr: 1.229 ± 0.025	1.88LysVal: 1.88 ± 0.036	0.296LysTrp: 0.296 ± 0.011	0.459LysTyr: 0.459 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.095LeuAla: 15.095 ± 0.12	0.798LeuCys: 0.798 ± 0.02	6.441LeuAsp: 6.441 ± 0.06	4.604LeuGlu: 4.604 ± 0.049	2.575LeuPhe: 2.575 ± 0.034	9.209LeuGly: 9.209 ± 0.079	2.015LeuHis: 2.015 ± 0.031	3.641LeuIle: 3.641 ± 0.043	1.906LeuLys: 1.906 ± 0.032	10.419LeuLeu: 10.419 ± 0.095	1.69LeuMet: 1.69 ± 0.026	1.803LeuAsn: 1.803 ± 0.029	6.262LeuPro: 6.262 ± 0.058	2.055LeuGln: 2.055 ± 0.029	8.826LeuArg: 8.826 ± 0.083	5.472LeuSer: 5.472 ± 0.046	6.66LeuThr: 6.66 ± 0.056	8.717LeuVal: 8.717 ± 0.075	1.334LeuTrp: 1.334 ± 0.026	1.917LeuTyr: 1.917 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.372MetAla: 2.372 ± 0.029	0.136MetCys: 0.136 ± 0.008	0.903MetAsp: 0.903 ± 0.019	0.797MetGlu: 0.797 ± 0.018	0.506MetPhe: 0.506 ± 0.014	1.373MetGly: 1.373 ± 0.026	0.319MetHis: 0.319 ± 0.011	0.858MetIle: 0.858 ± 0.018	0.419MetLys: 0.419 ± 0.013	1.859MetLeu: 1.859 ± 0.029	0.316MetMet: 0.316 ± 0.012	0.439MetAsn: 0.439 ± 0.012	1.19MetPro: 1.19 ± 0.023	0.398MetGln: 0.398 ± 0.012	1.651MetArg: 1.651 ± 0.029	1.305MetSer: 1.305 ± 0.022	1.513MetThr: 1.513 ± 0.023	1.418MetVal: 1.418 ± 0.022	0.221MetTrp: 0.221 ± 0.009	0.337MetTyr: 0.337 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.333AsnAla: 2.333 ± 0.036	0.185AsnCys: 0.185 ± 0.009	1.008AsnAsp: 1.008 ± 0.021	0.818AsnGlu: 0.818 ± 0.019	0.514AsnPhe: 0.514 ± 0.016	2.12AsnGly: 2.12 ± 0.045	0.385AsnHis: 0.385 ± 0.011	0.67AsnIle: 0.67 ± 0.019	0.401AsnLys: 0.401 ± 0.013	1.849AsnLeu: 1.849 ± 0.031	0.309AsnMet: 0.309 ± 0.012	0.498AsnAsn: 0.498 ± 0.019	1.456AsnPro: 1.456 ± 0.028	0.551AsnGln: 0.551 ± 0.018	1.325AsnArg: 1.325 ± 0.025	0.942AsnSer: 0.942 ± 0.021	1.149AsnThr: 1.149 ± 0.027	1.608AsnVal: 1.608 ± 0.024	0.317AsnTrp: 0.317 ± 0.011	0.474AsnTyr: 0.474 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.696ProAla: 8.696 ± 0.077	0.389ProCys: 0.389 ± 0.013	4.204ProAsp: 4.204 ± 0.045	4.17ProGlu: 4.17 ± 0.042	1.586ProPhe: 1.586 ± 0.025	7.27ProGly: 7.27 ± 0.07	1.266ProHis: 1.266 ± 0.023	1.835ProIle: 1.835 ± 0.028	1.195ProLys: 1.195 ± 0.026	5.192ProLeu: 5.192 ± 0.045	1.092ProMet: 1.092 ± 0.021	1.008ProAsn: 1.008 ± 0.022	4.204ProPro: 4.204 ± 0.061	1.561ProGln: 1.561 ± 0.031	4.194ProArg: 4.194 ± 0.051	3.618ProSer: 3.618 ± 0.047	3.136ProThr: 3.136 ± 0.047	5.311ProVal: 5.311 ± 0.053	0.963ProTrp: 0.963 ± 0.021	1.468ProTyr: 1.468 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.874GlnAla: 3.874 ± 0.05	0.169GlnCys: 0.169 ± 0.009	1.247GlnAsp: 1.247 ± 0.021	1.372GlnGlu: 1.372 ± 0.026	0.687GlnPhe: 0.687 ± 0.017	2.182GlnGly: 2.182 ± 0.032	0.517GlnHis: 0.517 ± 0.013	1.263GlnIle: 1.263 ± 0.024	0.577GlnLys: 0.577 ± 0.017	2.442GlnLeu: 2.442 ± 0.042	0.53GlnMet: 0.53 ± 0.016	0.535GlnAsn: 0.535 ± 0.016	1.508GlnPro: 1.508 ± 0.027	0.979GlnGln: 0.979 ± 0.026	2.255GlnArg: 2.255 ± 0.031	1.149GlnSer: 1.149 ± 0.022	1.397GlnThr: 1.397 ± 0.033	2.538GlnVal: 2.538 ± 0.034	0.478GlnTrp: 0.478 ± 0.017	0.555GlnTyr: 0.555 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	9.942ArgAla: 9.942 ± 0.092	0.614ArgCys: 0.614 ± 0.015	4.599ArgAsp: 4.599 ± 0.05	4.771ArgGlu: 4.771 ± 0.052	2.518ArgPhe: 2.518 ± 0.032	6.007ArgGly: 6.007 ± 0.055	2.203ArgHis: 2.203 ± 0.029	2.955ArgIle: 2.955 ± 0.035	1.511ArgLys: 1.511 ± 0.026	9.488ArgLeu: 9.488 ± 0.089	1.802ArgMet: 1.802 ± 0.029	1.362ArgAsn: 1.362 ± 0.028	5.332ArgPro: 5.332 ± 0.064	2.371ArgGln: 2.371 ± 0.031	8.343ArgArg: 8.343 ± 0.084	4.008ArgSer: 4.008 ± 0.04	4.721ArgThr: 4.721 ± 0.044	6.657ArgVal: 6.657 ± 0.056	1.423ArgTrp: 1.423 ± 0.026	1.962ArgTyr: 1.962 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.602SerAla: 6.602 ± 0.06	0.429SerCys: 0.429 ± 0.013	2.658SerAsp: 2.658 ± 0.039	2.404SerGlu: 2.404 ± 0.033	1.564SerPhe: 1.564 ± 0.027	6.118SerGly: 6.118 ± 0.054	1.006SerHis: 1.006 ± 0.02	1.694SerIle: 1.694 ± 0.028	0.983SerLys: 0.983 ± 0.021	4.801SerLeu: 4.801 ± 0.041	1.139SerMet: 1.139 ± 0.02	0.903SerAsn: 0.903 ± 0.022	3.614SerPro: 3.614 ± 0.046	1.222SerGln: 1.222 ± 0.026	3.851SerArg: 3.851 ± 0.038	3.015SerSer: 3.015 ± 0.042	3.082SerThr: 3.082 ± 0.043	4.169SerVal: 4.169 ± 0.04	0.937SerTrp: 0.937 ± 0.021	1.225SerTyr: 1.225 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	8.041ThrAla: 8.041 ± 0.069	0.478ThrCys: 0.478 ± 0.013	3.004ThrAsp: 3.004 ± 0.042	2.715ThrGlu: 2.715 ± 0.032	1.762ThrPhe: 1.762 ± 0.031	6.736ThrGly: 6.736 ± 0.065	1.064ThrHis: 1.064 ± 0.019	2.223ThrIle: 2.223 ± 0.035	1.079ThrLys: 1.079 ± 0.025	5.862ThrLeu: 5.862 ± 0.053	1.059ThrMet: 1.059 ± 0.022	1.078ThrAsn: 1.078 ± 0.024	4.363ThrPro: 4.363 ± 0.059	1.35ThrGln: 1.35 ± 0.025	4.17ThrArg: 4.17 ± 0.046	3.377ThrSer: 3.377 ± 0.047	3.83ThrThr: 3.83 ± 0.066	5.686ThrVal: 5.686 ± 0.071	0.989ThrTrp: 0.989 ± 0.022	1.423ThrTyr: 1.423 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	11.155ValAla: 11.155 ± 0.093	0.719ValCys: 0.719 ± 0.02	4.888ValAsp: 4.888 ± 0.045	4.727ValGlu: 4.727 ± 0.047	2.46ValPhe: 2.46 ± 0.034	6.371ValGly: 6.371 ± 0.057	1.875ValHis: 1.875 ± 0.028	3.423ValIle: 3.423 ± 0.039	1.815ValLys: 1.815 ± 0.032	9.179ValLeu: 9.179 ± 0.072	1.51ValMet: 1.51 ± 0.028	1.853ValAsn: 1.853 ± 0.035	5.489ValPro: 5.489 ± 0.052	2.031ValGln: 2.031 ± 0.028	7.167ValArg: 7.167 ± 0.059	4.593ValSer: 4.593 ± 0.046	6.109ValThr: 6.109 ± 0.061	8.247ValVal: 8.247 ± 0.079	1.121ValTrp: 1.121 ± 0.023	1.735ValTyr: 1.735 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.62TrpAla: 1.62 ± 0.026	0.157TrpCys: 0.157 ± 0.009	0.885TrpAsp: 0.885 ± 0.023	0.734TrpGlu: 0.734 ± 0.017	0.539TrpPhe: 0.539 ± 0.016	1.033TrpGly: 1.033 ± 0.024	0.412TrpHis: 0.412 ± 0.011	0.625TrpIle: 0.625 ± 0.018	0.371TrpLys: 0.371 ± 0.014	1.708TrpLeu: 1.708 ± 0.029	0.33TrpMet: 0.33 ± 0.01	0.452TrpAsn: 0.452 ± 0.016	0.892TrpPro: 0.892 ± 0.019	0.548TrpGln: 0.548 ± 0.015	1.461TrpArg: 1.461 ± 0.024	0.922TrpSer: 0.922 ± 0.022	1.078TrpThr: 1.078 ± 0.023	1.041TrpVal: 1.041 ± 0.023	0.375TrpTrp: 0.375 ± 0.012	0.363TrpTyr: 0.363 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.745TyrAla: 2.745 ± 0.033	0.187TyrCys: 0.187 ± 0.008	1.473TyrAsp: 1.473 ± 0.033	1.156TyrGlu: 1.156 ± 0.025	0.682TyrPhe: 0.682 ± 0.017	2.335TyrGly: 2.335 ± 0.036	0.462TyrHis: 0.462 ± 0.015	0.636TyrIle: 0.636 ± 0.017	0.426TyrLys: 0.426 ± 0.016	2.227TyrLeu: 2.227 ± 0.033	0.284TyrMet: 0.284 ± 0.011	0.499TyrAsn: 0.499 ± 0.017	1.143TyrPro: 1.143 ± 0.026	0.691TyrGln: 0.691 ± 0.018	1.895TyrArg: 1.895 ± 0.025	1.072TyrSer: 1.072 ± 0.023	1.311TyrThr: 1.311 ± 0.033	1.821TyrVal: 1.821 ± 0.028	0.378TyrTrp: 0.378 ± 0.013	0.527TyrTyr: 0.527 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7626 proteins (2573401 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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