Strongylus vulgaris (Blood worm)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P7J8P8|A0A3P7J8P8_STRVU Pyr_redox domain-containing protein OS=Strongylus vulgaris OX=40348 GN=SVUK_LOCUS16759 PE=4 SV=1
MM1 pKa = 7.2 GVEE4 pKa = 4.25 EE5 pKa = 4.75 SPAVTAAPQGVCCGCGVSPQGPPGPPGPDD34 pKa = 3.38 GEE36 pKa = 4.63 NGADD40 pKa = 3.92 GAPGAPGKK48 pKa = 10.19 DD49 pKa = 3.79 GPDD52 pKa = 3.63 GPLPTPPPQHH62 pKa = 6.29 EE63 pKa = 4.49 FCFDD67 pKa = 3.93 CPDD70 pKa = 4.23 GPPGAPGNPGPKK82 pKa = 9.89 GPPGPPGAPGADD94 pKa = 3.14 ADD96 pKa = 4.26 GGSRR100 pKa = 11.84 GPPGPPGPPGPPGPDD115 pKa = 3.18 GAPGNAGAPGAPGKK129 pKa = 10.54 LNEE132 pKa = 4.45 VPGPDD137 pKa = 4.46 GPPGPPGPQGPPGPDD152 pKa = 3.27 GQPGANGNPGQDD164 pKa = 3.64 GAPGQPGDD172 pKa = 4.63 DD173 pKa = 4.16 GAPGAPGQPGPAGPAGPDD191 pKa = 3.56 GDD193 pKa = 4.1 RR194 pKa = 11.84 GPSGGCDD201 pKa = 2.86 HH202 pKa = 7.24 CPPPRR207 pKa = 11.84 TAPGYY212 pKa = 10.37
Molecular weight: 19.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.503
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.164
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A3P7L5J8|A0A3P7L5J8_STRVU Uncharacterized protein OS=Strongylus vulgaris OX=40348 GN=SVUK_LOCUS9680 PE=4 SV=1
MM1 pKa = 6.74 KK2 pKa = 9.72 TKK4 pKa = 9.72 TRR6 pKa = 11.84 TRR8 pKa = 11.84 IRR10 pKa = 11.84 TKK12 pKa = 7.9 TKK14 pKa = 10.33 RR15 pKa = 11.84 RR16 pKa = 11.84 ITKK19 pKa = 7.79 TRR21 pKa = 11.84 TRR23 pKa = 11.84 IKK25 pKa = 9.69 MIRR28 pKa = 11.84 IKK30 pKa = 10.39 RR31 pKa = 11.84 KK32 pKa = 9.22 RR33 pKa = 11.84 KK34 pKa = 8.29 HH35 pKa = 4.12 QRR37 pKa = 11.84 KK38 pKa = 8.55 MIRR41 pKa = 11.84 KK42 pKa = 7.98 RR43 pKa = 11.84 KK44 pKa = 8.08 RR45 pKa = 11.84 IRR47 pKa = 11.84 RR48 pKa = 11.84 IKK50 pKa = 9.76 MMRR53 pKa = 11.84 LKK55 pKa = 10.54 SRR57 pKa = 11.84 SQIKK61 pKa = 10.07 SS62 pKa = 3.08
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.491
IPC_protein 13.071
Toseland 13.247
ProMoST 13.729
Dawson 13.247
Bjellqvist 13.232
Wikipedia 13.715
Rodwell 12.998
Grimsley 13.276
Solomon 13.729
Lehninger 13.642
Nozaki 13.247
DTASelect 13.232
Thurlkill 13.247
EMBOSS 13.744
Sillero 13.247
Patrickios 12.72
IPC_peptide 13.744
IPC2_peptide 12.72
IPC2.peptide.svr19 9.336
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20844
0
20844
3866246
27
2583
185.5
20.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.133 ± 0.018
2.089 ± 0.016
5.291 ± 0.015
6.642 ± 0.025
4.265 ± 0.016
5.8 ± 0.024
2.364 ± 0.01
5.55 ± 0.016
5.723 ± 0.023
9.106 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.727 ± 0.011
4.079 ± 0.012
5.138 ± 0.023
3.839 ± 0.016
5.956 ± 0.018
7.666 ± 0.023
5.505 ± 0.018
6.704 ± 0.021
1.139 ± 0.007
3.081 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here