Spinacia oleracea (Spinach)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23526 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9QH64|A0A0K9QH64_SPIOL Uncharacterized protein OS=Spinacia oleracea OX=3562 GN=SOVF_179700 PE=4 SV=1
AA1 pKa = 7.42TKK3 pKa = 10.12NSSMEE8 pKa = 4.13GEE10 pKa = 4.43TSSKK14 pKa = 9.78AQSVNSSSNSSADD27 pKa = 3.5KK28 pKa = 11.35NNMEE32 pKa = 5.14MIPFTGSVDD41 pKa = 2.92HH42 pKa = 7.11GYY44 pKa = 9.75PCFEE48 pKa = 4.57EE49 pKa = 5.66SNVAYY54 pKa = 10.71LEE56 pKa = 4.79PIPLNTYY63 pKa = 9.1YY64 pKa = 11.5NNMDD68 pKa = 3.97LQLLEE73 pKa = 4.79PPSFPTIPHH82 pKa = 6.86LQTDD86 pKa = 5.65LIPFPPEE93 pKa = 3.51TQEE96 pKa = 5.27LIDD99 pKa = 4.49RR100 pKa = 11.84LDD102 pKa = 3.39SSFFSPFGCTGEE114 pKa = 4.15PALVDD119 pKa = 4.18DD120 pKa = 6.0GISFYY125 pKa = 11.59SCFDD129 pKa = 3.15GDD131 pKa = 4.44AISGEE136 pKa = 4.08TRR138 pKa = 11.84VKK140 pKa = 10.63RR141 pKa = 11.84EE142 pKa = 3.86NYY144 pKa = 10.39NNATPDD150 pKa = 3.27SFFDD154 pKa = 4.33DD155 pKa = 4.58FPSDD159 pKa = 3.43VFDD162 pKa = 5.22EE163 pKa = 4.49IEE165 pKa = 4.44PLPSPSGWW173 pKa = 3.13

Molecular weight: 19.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K9RSJ2|A0A0K9RSJ2_SPIOL Dimer_Tnp_hAT domain-containing protein OS=Spinacia oleracea OX=3562 GN=SOVF_039210 PE=4 SV=1
MM1 pKa = 7.55AGGVRR6 pKa = 11.84RR7 pKa = 11.84SMINGGSGSQGSTTSLGRR25 pKa = 11.84PIPKK29 pKa = 9.6RR30 pKa = 11.84GQIKK34 pKa = 10.15GRR36 pKa = 11.84IVMGLAHH43 pKa = 6.42SVVAIFSHH51 pKa = 6.25ATPSHH56 pKa = 6.26RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 3.51

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21534

1992

23526

9276580

8

5409

394.3

43.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.574 ± 0.015

1.853 ± 0.007

5.454 ± 0.012

6.483 ± 0.017

4.132 ± 0.01

6.593 ± 0.02

2.365 ± 0.007

5.273 ± 0.011

6.183 ± 0.018

9.543 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.007

4.572 ± 0.011

4.929 ± 0.017

3.732 ± 0.013

5.192 ± 0.013

9.014 ± 0.019

4.956 ± 0.009

6.67 ± 0.011

1.231 ± 0.006

2.826 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski