Lymantria dispar cypovirus 14
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91IE6|Q91IE6_9REOV Uncharacterized protein OS=Lymantria dispar cypovirus 14 OX=165429 PE=4 SV=1
MM1 pKa = 7.47 SISRR5 pKa = 11.84 SEE7 pKa = 3.68 ITDD10 pKa = 2.8 IGGRR14 pKa = 11.84 LLYY17 pKa = 9.24 TVYY20 pKa = 9.53 YY21 pKa = 6.85 TTLDD25 pKa = 3.23 NYY27 pKa = 11.37 KK28 pKa = 10.35 LIKK31 pKa = 10.72 NEE33 pKa = 3.85 FTSNGLVHH41 pKa = 6.85 KK42 pKa = 10.23 AVNSKK47 pKa = 10.84 ALTNVRR53 pKa = 11.84 MLIEE57 pKa = 4.61 NFTKK61 pKa = 10.66 KK62 pKa = 10.34 NINIIYY68 pKa = 9.63 IEE70 pKa = 4.05 QGSILASNITTLALTYY86 pKa = 11.09 KK87 pKa = 9.24 MLIVIQSPKK96 pKa = 10.07 EE97 pKa = 3.75 KK98 pKa = 9.84 TIAGAIKK105 pKa = 8.88 WRR107 pKa = 11.84 NAVGSRR113 pKa = 11.84 INGPVHH119 pKa = 6.08 MALSDD124 pKa = 3.9 YY125 pKa = 10.7 IALIKK130 pKa = 10.54 AEE132 pKa = 4.18 RR133 pKa = 11.84 EE134 pKa = 3.92 KK135 pKa = 10.91 AVQSSTVEE143 pKa = 4.0 PVSVVPVAKK152 pKa = 10.55 LMQEE156 pKa = 4.07 PVHH159 pKa = 8.01 VDD161 pKa = 3.0 VTQAEE166 pKa = 4.24 VHH168 pKa = 5.78 IQNEE172 pKa = 4.47 EE173 pKa = 4.12 VKK175 pKa = 10.45 VDD177 pKa = 3.25 HH178 pKa = 6.65 SVNEE182 pKa = 3.97 EE183 pKa = 4.07 SAPSDD188 pKa = 3.18 SVTEE192 pKa = 3.97 VKK194 pKa = 9.97 QQNVCSTCDD203 pKa = 3.33 KK204 pKa = 10.66 PIAEE208 pKa = 4.6 YY209 pKa = 11.25 ACMRR213 pKa = 11.84 DD214 pKa = 5.72 DD215 pKa = 5.17 NDD217 pKa = 4.18 CSCDD221 pKa = 4.12 AMDD224 pKa = 5.57 DD225 pKa = 3.84 ALNKK229 pKa = 10.61 SFDD232 pKa = 4.21 TIDD235 pKa = 3.43 SDD237 pKa = 5.15 GATLDD242 pKa = 4.07 LPALSMRR249 pKa = 11.84 RR250 pKa = 11.84 VYY252 pKa = 11.14 DD253 pKa = 3.69 SVCIGCADD261 pKa = 3.67 APLEE265 pKa = 3.78 QLVLHH270 pKa = 6.76 IEE272 pKa = 4.1 LSEE275 pKa = 4.0 LMTRR279 pKa = 11.84 ARR281 pKa = 11.84 LLLSKK286 pKa = 10.23 FMKK289 pKa = 10.4 VYY291 pKa = 10.65 LVPSDD296 pKa = 4.82 LDD298 pKa = 3.64 WNEE301 pKa = 3.96 LRR303 pKa = 11.84 TITIDD308 pKa = 4.99 GITLQYY314 pKa = 9.64 RR315 pKa = 11.84 TTLKK319 pKa = 10.68 DD320 pKa = 3.5 SNSILCEE327 pKa = 3.78 SEE329 pKa = 5.3 LISKK333 pKa = 8.9 VFEE336 pKa = 4.05 MYY338 pKa = 10.27 RR339 pKa = 11.84 GSEE342 pKa = 3.87 RR343 pKa = 11.84 LFQYY347 pKa = 10.83 KK348 pKa = 10.24 DD349 pKa = 3.42 QLCSMIGMHH358 pKa = 6.83 ISGDD362 pKa = 3.59 PTIFICSNACGLMLAKK378 pKa = 9.55 MM379 pKa = 4.32
Molecular weight: 42.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.045
IPC2_protein 5.143
IPC_protein 5.092
Toseland 5.041
ProMoST 5.245
Dawson 5.105
Bjellqvist 5.219
Wikipedia 4.978
Rodwell 5.016
Grimsley 4.965
Solomon 5.092
Lehninger 5.054
Nozaki 5.232
DTASelect 5.397
Thurlkill 5.067
EMBOSS 5.041
Sillero 5.296
Patrickios 3.834
IPC_peptide 5.105
IPC2_peptide 5.296
IPC2.peptide.svr19 5.278
Protein with the highest isoelectric point:
>tr|Q91IE8|Q91IE8_9REOV Uncharacterized protein OS=Lymantria dispar cypovirus 14 OX=165429 PE=4 SV=1
MM1 pKa = 7.5 LAIDD5 pKa = 3.74 YY6 pKa = 8.23 LQNEE10 pKa = 4.73 RR11 pKa = 11.84 LYY13 pKa = 9.12 HH14 pKa = 5.57 TYY16 pKa = 9.5 MLKK19 pKa = 9.55 TKK21 pKa = 9.97 TNEE24 pKa = 3.83 NNNAPTPEE32 pKa = 3.97 VKK34 pKa = 10.28 QFLLQSSIAHH44 pKa = 6.46 KK45 pKa = 10.52 LLRR48 pKa = 11.84 AYY50 pKa = 10.69 SNKK53 pKa = 8.92 TKK55 pKa = 10.8 GVIKK59 pKa = 10.63 DD60 pKa = 3.55 PALLGAIISTGSYY73 pKa = 10.52 DD74 pKa = 3.54 VQVSNKK80 pKa = 8.98 KK81 pKa = 9.7 DD82 pKa = 3.27 RR83 pKa = 11.84 KK84 pKa = 9.72 IVGKK88 pKa = 10.31 AIDD91 pKa = 3.92 GANVKK96 pKa = 8.46 VTVKK100 pKa = 10.4 KK101 pKa = 10.44 GRR103 pKa = 11.84 KK104 pKa = 8.83 KK105 pKa = 10.19 RR106 pKa = 11.84 DD107 pKa = 3.04 VMADD111 pKa = 3.37 MPAQTSLAADD121 pKa = 3.46 QEE123 pKa = 4.91 GVLTLEE129 pKa = 4.96 DD130 pKa = 3.9 MNEE133 pKa = 3.83 LSEE136 pKa = 4.18 MAKK139 pKa = 10.38 KK140 pKa = 10.45 LRR142 pKa = 11.84 TVYY145 pKa = 9.07 NTSEE149 pKa = 4.45 SKK151 pKa = 10.74 SLWYY155 pKa = 10.04 IKK157 pKa = 9.67 DD158 pKa = 3.19 EE159 pKa = 4.51 RR160 pKa = 11.84 YY161 pKa = 11.22 SMLIPSYY168 pKa = 9.14 ITAIMPFMPRR178 pKa = 11.84 RR179 pKa = 11.84 EE180 pKa = 4.04 IEE182 pKa = 3.93 LSEE185 pKa = 4.38 KK186 pKa = 10.61 GRR188 pKa = 11.84 MVVPLDD194 pKa = 3.55 LKK196 pKa = 11.03 HH197 pKa = 6.12 IFRR200 pKa = 11.84 NIVVVRR206 pKa = 11.84 EE207 pKa = 4.0 YY208 pKa = 11.47 NEE210 pKa = 3.71 NQLRR214 pKa = 11.84 NNKK217 pKa = 8.74 PCNSLLINASVLSDD231 pKa = 3.38 SVYY234 pKa = 9.1 MQKK237 pKa = 10.67 GYY239 pKa = 9.86 QVFTNSVIVYY249 pKa = 5.91 TASWRR254 pKa = 11.84 NRR256 pKa = 11.84 EE257 pKa = 3.4 VDD259 pKa = 5.27 FIMFDD264 pKa = 3.28 TRR266 pKa = 11.84 NVVDD270 pKa = 5.6 NIDD273 pKa = 3.79 GSDD276 pKa = 3.63 VEE278 pKa = 4.93 IVWKK282 pKa = 10.27 EE283 pKa = 3.63 LIKK286 pKa = 10.6 YY287 pKa = 7.81 ARR289 pKa = 11.84 PVANLYY295 pKa = 7.7 EE296 pKa = 4.26 TLEE299 pKa = 4.19 PEE301 pKa = 4.1 MVIKK305 pKa = 10.76 KK306 pKa = 9.87 KK307 pKa = 10.87 GPTIYY312 pKa = 10.49 QPIRR316 pKa = 11.84 GATYY320 pKa = 10.45 AVLLKK325 pKa = 10.57 FRR327 pKa = 11.84 EE328 pKa = 4.13 QCLSKK333 pKa = 9.33 TQLKK337 pKa = 10.72 VLMTFIGNKK346 pKa = 9.92 GFGEE350 pKa = 4.41 TSFLRR355 pKa = 11.84 DD356 pKa = 3.31 VIAALNSKK364 pKa = 10.45 LGDD367 pKa = 3.61 NAAGAIDD374 pKa = 4.06 SDD376 pKa = 4.02 WYY378 pKa = 10.88 GIWEE382 pKa = 4.24 YY383 pKa = 11.26 KK384 pKa = 9.08 RR385 pKa = 11.84 TVEE388 pKa = 4.97 GIEE391 pKa = 4.6 LPLEE395 pKa = 4.08 YY396 pKa = 10.18 NVCVEE401 pKa = 4.13 QLEE404 pKa = 4.53 SGPSIFEE411 pKa = 4.21 YY412 pKa = 10.61 YY413 pKa = 10.72 AEE415 pKa = 4.69 QILSAANIKK424 pKa = 9.62 TSEE427 pKa = 4.18 QYY429 pKa = 10.83 FKK431 pKa = 10.99 TKK433 pKa = 9.43 IQQRR437 pKa = 11.84 EE438 pKa = 3.98 TMINEE443 pKa = 4.35 CKK445 pKa = 10.62 SKK447 pKa = 11.18 VEE449 pKa = 3.89 QHH451 pKa = 5.61 WLNGKK456 pKa = 8.47 QSPEE460 pKa = 3.6 YY461 pKa = 9.61 QFNVRR466 pKa = 11.84 VTTSKK471 pKa = 8.94 TAPRR475 pKa = 11.84 ILILNQHH482 pKa = 5.98 TVTQDD487 pKa = 3.11 VTAGRR492 pKa = 11.84 SDD494 pKa = 2.98 INLILKK500 pKa = 8.78 PIIDD504 pKa = 4.35 PVSMLLLRR512 pKa = 11.84 DD513 pKa = 3.16 RR514 pKa = 11.84 AMPAEE519 pKa = 4.07 LLLYY523 pKa = 7.34 EE524 pKa = 4.39 TYY526 pKa = 11.57 GMLTSYY532 pKa = 10.61 VHH534 pKa = 6.13 TSMYY538 pKa = 10.61 ACEE541 pKa = 4.11 LLRR544 pKa = 11.84 FIEE547 pKa = 4.3 YY548 pKa = 10.17 DD549 pKa = 2.94 WW550 pKa = 5.68
Molecular weight: 63.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.702
IPC2_protein 7.966
IPC_protein 7.863
Toseland 8.126
ProMoST 8.346
Dawson 8.682
Bjellqvist 8.697
Wikipedia 8.756
Rodwell 8.77
Grimsley 8.258
Solomon 8.785
Lehninger 8.741
Nozaki 8.726
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.843
Sillero 8.858
Patrickios 4.329
IPC_peptide 8.77
IPC2_peptide 7.366
IPC2.peptide.svr19 7.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
7925
233
1267
720.5
81.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.562 ± 0.234
1.186 ± 0.323
6.019 ± 0.232
5.716 ± 0.255
3.899 ± 0.214
4.808 ± 0.27
2.259 ± 0.17
6.524 ± 0.407
4.656 ± 0.572
9.274 ± 0.413
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.331 ± 0.165
6.057 ± 0.456
3.836 ± 0.313
3.95 ± 0.321
6.145 ± 0.3
7.155 ± 0.217
6.448 ± 0.332
7.066 ± 0.561
0.782 ± 0.065
4.328 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here