Paramecium bursaria Chlorella virus FR483 (PBCV-FR483)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus; Paramecium bursaria Chlorella virus A1

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7J7U3|A7J7U3_PBCVF Uncharacterized protein N589L OS=Paramecium bursaria Chlorella virus FR483 OX=399781 GN=N589L PE=4 SV=1
MM1 pKa = 7.81VFEE4 pKa = 5.03GDD6 pKa = 3.74SVKK9 pKa = 11.23DD10 pKa = 3.39MLILYY15 pKa = 7.54STLVGTNTSAEE26 pKa = 4.22EE27 pKa = 4.23TEE29 pKa = 4.43HH30 pKa = 6.79LFKK33 pKa = 11.12KK34 pKa = 10.4LVEE37 pKa = 4.35SVQAIDD43 pKa = 3.98PDD45 pKa = 4.55FVPHH49 pKa = 7.2LGTSLTAYY57 pKa = 9.34EE58 pKa = 4.45AFLLVEE64 pKa = 4.43KK65 pKa = 9.76DD66 pKa = 3.5TYY68 pKa = 10.8SGNVTALLGDD78 pKa = 3.45IEE80 pKa = 5.78GYY82 pKa = 9.48TDD84 pKa = 3.01TTMEE88 pKa = 4.12YY89 pKa = 9.5TVWDD93 pKa = 4.64PIVPVDD99 pKa = 4.78DD100 pKa = 3.95TQQYY104 pKa = 10.43PDD106 pKa = 4.17DD107 pKa = 4.25EE108 pKa = 4.59CVEE111 pKa = 4.26RR112 pKa = 11.84AVTPAIPCVLPEE124 pKa = 4.12RR125 pKa = 11.84DD126 pKa = 3.7YY127 pKa = 11.55HH128 pKa = 8.04VDD130 pKa = 3.24TSPEE134 pKa = 3.92FFQGDD139 pKa = 4.5DD140 pKa = 3.34IVGLLSDD147 pKa = 3.79EE148 pKa = 4.31MEE150 pKa = 4.82EE151 pKa = 3.69ISLFDD156 pKa = 3.22EE157 pKa = 4.81HH158 pKa = 7.43KK159 pKa = 10.54PNNDD163 pKa = 2.94TDD165 pKa = 3.83QYY167 pKa = 11.51PEE169 pKa = 4.12DD170 pKa = 4.18TNVSIVDD177 pKa = 3.67RR178 pKa = 11.84SAFSNEE184 pKa = 3.19YY185 pKa = 10.05AARR188 pKa = 11.84DD189 pKa = 4.26FII191 pKa = 6.15

Molecular weight: 21.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7J790|A7J790_PBCVF Uncharacterized protein n386R OS=Paramecium bursaria Chlorella virus FR483 OX=399781 GN=n386R PE=4 SV=1
MM1 pKa = 6.92STWKK5 pKa = 9.91PSLMKK10 pKa = 10.36RR11 pKa = 11.84RR12 pKa = 11.84TVRR15 pKa = 11.84AMVMMWVSRR24 pKa = 11.84PLMKK28 pKa = 10.52LFSLIMRR35 pKa = 11.84KK36 pKa = 7.52MRR38 pKa = 11.84KK39 pKa = 8.31IRR41 pKa = 11.84KK42 pKa = 7.69SRR44 pKa = 11.84KK45 pKa = 7.14IRR47 pKa = 11.84KK48 pKa = 8.15SRR50 pKa = 11.84KK51 pKa = 7.17IRR53 pKa = 11.84KK54 pKa = 7.17SRR56 pKa = 11.84KK57 pKa = 5.84MRR59 pKa = 11.84KK60 pKa = 7.91SRR62 pKa = 11.84KK63 pKa = 6.63IRR65 pKa = 11.84KK66 pKa = 6.67MRR68 pKa = 11.84KK69 pKa = 7.0SRR71 pKa = 11.84KK72 pKa = 7.7IRR74 pKa = 11.84KK75 pKa = 7.03SRR77 pKa = 11.84KK78 pKa = 5.85MRR80 pKa = 11.84RR81 pKa = 11.84LRR83 pKa = 11.84KK84 pKa = 8.82MRR86 pKa = 11.84RR87 pKa = 11.84LRR89 pKa = 11.84KK90 pKa = 8.33MRR92 pKa = 11.84KK93 pKa = 6.65FRR95 pKa = 11.84KK96 pKa = 8.02MRR98 pKa = 11.84KK99 pKa = 8.02LRR101 pKa = 11.84RR102 pKa = 11.84MLYY105 pKa = 9.97LRR107 pKa = 11.84FRR109 pKa = 11.84MICLMRR115 pKa = 11.84KK116 pKa = 7.28TIPFGITRR124 pKa = 11.84MFLLWW129 pKa = 3.83

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

848

0

848

151029

65

1612

178.1

19.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.697 ± 0.118

1.994 ± 0.073

4.591 ± 0.105

4.369 ± 0.136

4.894 ± 0.102

6.458 ± 0.219

2.208 ± 0.082

6.421 ± 0.12

6.01 ± 0.201

8.693 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.072 ± 0.076

5.075 ± 0.178

5.201 ± 0.197

2.9 ± 0.07

5.212 ± 0.132

8.226 ± 0.144

6.521 ± 0.131

7.07 ± 0.109

1.247 ± 0.046

3.142 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski