Streptomyces phage Wentworth

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N0R9|A0A2P1N0R9_9CAUD Uncharacterized protein OS=Streptomyces phage Wentworth OX=2126735 GN=96 PE=4 SV=1
MM1 pKa = 7.62IPDD4 pKa = 4.05PEE6 pKa = 4.99RR7 pKa = 11.84EE8 pKa = 4.15LMDD11 pKa = 5.89DD12 pKa = 5.5DD13 pKa = 5.13SCQLCEE19 pKa = 4.06EE20 pKa = 4.7APDD23 pKa = 3.74AEE25 pKa = 4.38MGLFIDD31 pKa = 5.16RR32 pKa = 11.84EE33 pKa = 4.23NDD35 pKa = 3.01NTTVWSHH42 pKa = 5.67AQCGYY47 pKa = 10.99DD48 pKa = 5.26ADD50 pKa = 4.22LQLAA54 pKa = 4.03

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N0N6|A0A2P1N0N6_9CAUD Uncharacterized protein OS=Streptomyces phage Wentworth OX=2126735 GN=51 PE=4 SV=1
MM1 pKa = 7.46GFVPLPSDD9 pKa = 3.77EE10 pKa = 4.58EE11 pKa = 4.51VVKK14 pKa = 10.36AWRR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 4.49GEE21 pKa = 4.01NNTRR25 pKa = 11.84TAEE28 pKa = 3.95AFGTNEE34 pKa = 3.64SAIRR38 pKa = 11.84KK39 pKa = 8.78AVMRR43 pKa = 11.84ATGRR47 pKa = 11.84MPRR50 pKa = 11.84SYY52 pKa = 10.88KK53 pKa = 10.32RR54 pKa = 11.84LPLTGAQLYY63 pKa = 10.14GAFLKK68 pKa = 10.84EE69 pKa = 4.14GADD72 pKa = 3.5RR73 pKa = 11.84GAMTRR78 pKa = 11.84LARR81 pKa = 11.84RR82 pKa = 11.84WDD84 pKa = 3.69AKK86 pKa = 10.07PQTVSAAIRR95 pKa = 11.84RR96 pKa = 11.84YY97 pKa = 9.61LDD99 pKa = 3.24KK100 pKa = 11.26VDD102 pKa = 3.87AVV104 pKa = 3.62

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

20292

33

1443

197.0

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.984 ± 0.375

1.178 ± 0.144

7.363 ± 0.234

7.022 ± 0.425

2.893 ± 0.148

8.092 ± 0.222

1.976 ± 0.178

4.465 ± 0.198

5.224 ± 0.358

7.688 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.179

3.519 ± 0.18

4.662 ± 0.213

3.755 ± 0.158

6.155 ± 0.351

5.613 ± 0.258

5.973 ± 0.486

6.801 ± 0.306

1.937 ± 0.158

2.804 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski